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Plant Molecular Biology

, Volume 26, Issue 6, pp 1995–2001 | Cite as

The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules

  • Carolina Carsolio
  • Francisco Campos
  • Federico Sánchez
  • Mario Rocha-Sosa
Short Communication

Abstract

In Phaseolus vulgaris there is a nodulin family, Npv30, of ca. 30 kDa, as detected in an in vitro translation assay [2]. We isolated a gene (npv30-1) for one of the members of this family. The nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. The promoter was fused to the GUS reporter gene; this chimeric fusion was introduced into Lotus corniculatus via Agrobacterium rhizogenes transformation. GUS activity was only detected in the infected cells of the nodules of transgenic plants. By contrast, the expression of a 35S-GUS construct was restricted to the uninfected cells and the vascular tissue.

Key words

gene regulation nodule nodulin Phaseolus vulgaris transgenic plants 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Carolina Carsolio
    • 1
  • Francisco Campos
    • 1
  • Federico Sánchez
    • 1
  • Mario Rocha-Sosa
    • 1
  1. 1.Instituto de BiotecnologíaUniversidad Nacional Autónoma de MéxicoCuernavacaMéxico

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