Plant Molecular Biology

, Volume 26, Issue 6, pp 1737–1746

Molecular characterization of rice genes specifically expressed in the anther tapetum

  • Tohru Tsuchiya
  • Kinya Toriyama
  • Shin-ichiro Ejiri
  • Kokichi Hinata
Research Article

Abstract

In situ localization of mRNA was carried out on two cDNAs (Osc4 and Osc6) that had been isolated from rice anthers at the microspore stage. The mRNA corresponding to each cDNA was shown to be localized only in the tapetal cells of the rice immature anthers, but not in the microspores or the mature pollen. The corresponding genomic clone, Osg6B, was isolated, and its 5′-upstream region was found to regulate β-glucuronidase expression in the tapetum of transgenic tobacco. A set of 5′ deletions was also generated and a 1095 bp 5′ region was revealed to be necessary for activation of the Osg6B promoter in transgenic tobacco.

Key words

anther in situ hybridization promoter analysis rice tapetum transgenic tobacco 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Tohru Tsuchiya
    • 1
    • 2
  • Kinya Toriyama
    • 2
  • Shin-ichiro Ejiri
    • 1
  • Kokichi Hinata
    • 2
  1. 1.Institute for Cell Biology and Genetics, Faculty of AgricultureIwate UniversityMoriokaJapan
  2. 2.Laboratory of Plant Breeding, Faculty of AgricultureTohoku UniversitySendaiJapan

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