Plant Molecular Biology

, Volume 31, Issue 4, pp 937–943 | Cite as

The rpl5-rps 14-cob gene arrangement in Solanum tuberosum: rps14 is a transcribed and unedited pseudogene

  • Verónica Quiñones
  • Silvana Zanlungo
  • Alejandra Moenne
  • Isabel Gómez
  • Loreto Holuigue
  • Simon Litvak
  • Xavier Jordana
Short Communication


The L5 ribosomal protein gene (rpl5) and a S14 ribosomal protein pseudogene were identified by sequence analysis in the potato mitochondrial genome. The two genes are separated by one nucleotide and are found upstream of the apocytochrome b gene (cob), an arrangement conserved also in Arabidopsis and Brassica. The rpl5 gene has an intact open reading frame while the rpsl4 locus is disrupted by a five nucleotide duplication that introduces a frameshift in the reading frame. Editing of rpl5 and pseudorps14 cotranscripts has been studied by cDNA sequence analysis. Eight C residues are edited into U in the rpl5 coding region, resulting in eight amino acid changes that increase the homology between potato and other RPL5 polypeptides. Interestingly, the rps14 pseudogene sequence is not edited at any nucleotide position.

Key words

apocytochrome b gene plant mitochondria potato RNA editing rpl5 gene rpsl4 pseudogene 


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  1. 1.
    Aubert D, Bisanz-Seyer C, Herzog M: Mitochondrial rps14 is a transcribed and edited pseudogene in Arabidopsis thaliana. Plant Mol Biol 20: 1169–1174 (1992).Google Scholar
  2. 2.
    Bland MM, Levings III CS, Matzinger DF: The tobacco mitochondrial ATPase subunit 9 gene is closely linked to an open reading frame for a ribosomal protein. Mol Gen Genet 204: 8–16 (1986).Google Scholar
  3. 3.
    Bock H, Brennicke A, Schuster W: rps3 and rpl16 genes do not overlap in Oenothera mitochondria: GTG as a potential translation initiation codon in plant mitochondria? Plant Mol Biol 24: 811–818 (1994).Google Scholar
  4. 4.
    Bonen L: The mitochondrial genome: so simple yet so complex. Curr Opin Gen Devel 1: 515–522 (1991).Google Scholar
  5. 5.
    Brandt P, Unseld M, Eckert-Ossenkopp U, Brennicke A: An rps14 pseudogene is transcribed and edited in Arabidopsis mitochondria. Curr Genet 24: 330–336 (1993).Google Scholar
  6. 6.
    Dell'Orto P, Moenne A, Graves PV, Jordana X: The potato mitochondrial ATP synthase subunit 9: gene structure, RNA editing and partial protein sequence. Plant Sci 88: 45–53 (1993).Google Scholar
  7. 7.
    González DH, Bonnard G, Grienenberger J-M: A gene involved in the biogenesis of cytochromes is co-transcribed with a ribosomal protein gene in wheat mitochondria. Curr Genet 24: 248–255 (1993).Google Scholar
  8. 8.
    Grohmann L, Brennicke A, Schuster W: The mitochondrial gene encoding ribosomal protein S12 has been translocated to the nuclear genome in Oenothera. Nucl Acids Res 20: 5641–5646 (1992).Google Scholar
  9. 9.
    Grohmann L, Brennicke A, Schuster W: Genes for mitochondrial ribosomal proteins in plants. In: Nierhaus KH, Franceschi F, Subramanian AR, Erdmann VA, Wittmann-Liebold B (eds) The Translational Apparatus, Structure, Function, Regulation, Evolution, pp. 599–607. Plenum Press, New York/London (1993).Google Scholar
  10. 10.
    Gualberto JM, Wintz H, Weil JH, Grienenberger JM: The genes coding for subunit 3 of NADH dehydrogenase and for ribosomol protein S12 are present in wheat and maize mitochondrial genomes and are co-transcribed. Mol Gen Genet 215: 118–127 (1988).Google Scholar
  11. 11.
    Hunt MD, Newton KJ: The NCS3 mutation: genetic evidence for the expression of ribosomal protein genes in Zea mays mitochondria. EMBO J 10: 1045–1052 (1991).Google Scholar
  12. 12.
    Klein M, Eckert-Ossenkopp U, Schmiedeberg I, Brandt P, Unseld M, Brennicke A, Schuster W: Physical mapping of the mitochondrial genome of Arabidopsis thaliana by cosmid and YAC clones. Plant J 6: 447–455 (1994).Google Scholar
  13. 13.
    Knoop V, Ehrhardt T, Lättig K, Brennicke A: The gene for ribosomal protein S10 is present in mitochondria of pea and potato but absent from those of Arabidopsis and Oenothera. Curr Genet 27: 559–564 (1995).Google Scholar
  14. 14.
    Levings CS, Brown GG: Molecular biology of plant mitochondria. Cell 56: 171–179 (1989).Google Scholar
  15. 15.
    Oda K, Yamoto K, Ohta E, Nakamura Y, Takemura M, Nozato N, Akashi K, Kanegae T, Ogura Y, Kohchi T, Ohyama K: Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. J Mol Biol 223: 1–7 (1992).Google Scholar
  16. 16.
    Schuster W: Ribosomal protein gene rpl5 is cotranscribed with the nad3 gene in Oenothera mitochondria. Mol Gen Genet 240: 445–449 (1993).Google Scholar
  17. 17.
    Schuster W, Unseld M, Wissinger B, Brennicke A: Ribosomal protein S14 transcripts are edited in Oenothera mitochondria. Nucl Acids Res 18: 229–233 (1990).Google Scholar
  18. 18.
    Schuster W, Brennicke A: RNA editing makes mistakes in plant mitochondria: editing loses sense in transcripts of a rps19 pseudogene and in creating stop codons in coxl and rps3 mRNAs of Oenothera. Nucl Acids Res 19: 6923–6928 (1991).Google Scholar
  19. 19.
    Sutton CA, Conklin PL, Pruitt KD, Calfee AJ, Cobb AG, Hanson M: Editing of rps3/rpl16 transcripts creates a premature truncation of the rpl16 open reading frame. Curr Genet 23: 472–476 (1993).Google Scholar
  20. 20.
    Takemura M, Oda K, Yamato K, Ohta E, Nakamura Y, Nozato N, Akashi K, Ohyama K: Gene clusters for ribosomal proteins in the mitochondrial genome of a liverwort, Marchantia polymorpha. Nucl Acids Res 20: 3199–3205 (1992).Google Scholar
  21. 21.
    Wahleithner JA, Wolstenholme DR: Ribosomal protein S14 genes in broad bean mitochondrial DNA. Nucl Acids Res 16: 6897–6913 (1988).Google Scholar
  22. 22.
    Ye F, Bernhardt J, Abel WO: Genes for ribosomal proteins S3, L16, L5 and S14 are clustered in the mitochondrial genome of Brassica napus L. Curr Genet 24: 323–329 (1993).Google Scholar
  23. 23.
    Zanlungo S, Litvak S, Jordana X: Isolation and nucleotide sequence of the potato mitochondrial gene for apocytochrome b. Plant Mol Biol 17: 527–530 (1991).Google Scholar
  24. 24.
    Zanlungo S, Quiñones V, Moenne A, Holuigue L, Jordana X: A ribosomal protein S10 is found in the mitochondrial genome in Solanum tuberosum. Plant Mol Biol 25: 743–749 (1994).Google Scholar
  25. 25.
    Zanlungo S, Quiñones V, Moenne A, Holuigue L, Jordana X: Splicing and editing of rps10 transcripts in potato mitochondria. Curr Genet 27: 565–571 (1995).Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Verónica Quiñones
    • 1
  • Silvana Zanlungo
    • 1
  • Alejandra Moenne
    • 1
  • Isabel Gómez
    • 1
  • Loreto Holuigue
    • 1
  • Simon Litvak
    • 2
  • Xavier Jordana
    • 1
  1. 1.Departamento de Biología Celular y Molecular, Facultad de Ciencias BiológicasP. Universidad Católica de ChileSantiagoChile
  2. 2.Institut de Biochimie et Génétique CellulairesC.N.R.S.BordeauxFrance

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