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Plant Molecular Biology

, Volume 23, Issue 3, pp 567–581 | Cite as

Activity of a maize ubiquitin promoter in transgenic rice

  • Maria-Jesús Cornejo
  • Diane Luth
  • Kathleen M. Blankenship
  • Olin D. Anderson
  • Ann E. Blechl
Research Article

Abstract

We have used the maize ubiquitin 1 promoter, first exon and first intron (UBI) for rice (Oryza sativa L. cv. Taipei 309) transformation experiments and studied its expression in transgenic calli and plants. UBI directed significantly higher levels of transient gene expression than other promoter/intron combinations used for rice transformation. We exploited these high levels of expression to identify stable transformants obtained from callus-derived protoplasts co-transfected with two chimeric genes. The genes consisted of UBI fused to the coding regions of the uidA and bar marker genes (UBI:GUS and UBI:BAR). UBI:GUS expression increased in response to thermal stress in both transfected protoplasts and transgenic rice calli. Histochemical localization of GUS activity revealed that UBI was most active in rapidly dividing cells. This promoter is expressed in many, but not all, rice tissues and undergoes important changes in activity during the development of transgenic rice plants.

Key words

GUS expression heat shock Oryza sativa L. rice transformation ubiquitin 

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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Maria-Jesús Cornejo
    • 1
  • Diane Luth
    • 1
  • Kathleen M. Blankenship
    • 1
  • Olin D. Anderson
    • 1
  • Ann E. Blechl
    • 1
  1. 1.Western Regional Research Center, Agricultural Research ServiceUnited States Department of AgricultureAlbanyUSA

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