Plant Molecular Biology

, Volume 18, Issue 5, pp 897–908 | Cite as

Characterization of a Gy4 glycinin gene from soybean Glycine max cv. Forrest

  • Zhong-Tian Xue
  • Mei-Lin Xu
  • Wei Shen
  • Nai-Liang Zhuang
  • Wei-Ming Hu
  • San Chiun Shen
Research Article

Abstract

The glycinin gene family encoding the glycinin subunits in soybean plants is composed of at least five gene members. A genomic clone λS312 containing the Gy4 gene from a genomic library of cv. Forrest was isolated and partially characterized. The organization of this gene was found to be similar to that of a null allele from cv. Raiden, but different from the Gy4 gene from cv. Dare. The complete nucleotide sequence of this gene has been determined. It is 2599 bp long consisting of four exons and three introns. Comparing the DNA sequences between this gene and the gene from Dare and a null allele from Raiden, the difference found in the coding region was 5′-GCAGTGCAAG-3′ (nt 824 to 833) in the former case versus 5′-TGGAGTTGCAATT-3′ (nt 1314 to 1326) in the latter case in the exon 2 domain, resulting in three amino acid differences and one amino acid absence. Some other differences were also found in the non-coding region. The coding sequence and 5′-flanking region of the Gy4 gene, when compared with that of other legumin genes as well as group 1 glycinin subunit genes, revealed some interesting features: (1) a transposable element-like sequence was found in the hypervariable region (HVR) of the exon 3 domain, which was lacking in the legumin and the glycinin group 1 genes; (2) in the 5′-flanking region from nt −145 to −1, two high-homology sequences were found: one from nt −141 to nt −132, the other from nt −118 to nt −92 which includes the ‘legumin box’ and the RY repeat element.

Key words

divergence DNA sequence gene organization glycinin gene soybean transposable element 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    ABI: Model 370-A DNA Sequencing System User's Manual.Google Scholar
  2. 2.
    Argos P, Narayana SVL, Nielsen NC: Structural similarity between legumin and vicilin storage proteins from legumes. EMBO J 4: 1111–1117 (1985).PubMedGoogle Scholar
  3. 3.
    Barton KA, Tompson JF, Madison JT, Rosenthal R, Jarvis NP, Beachy RN: The biosynthesis and processing of high molecular weight procursors of soybean glycinin subunits. J Biol Chem 257: 6089–6095 (1982).PubMedGoogle Scholar
  4. 4.
    Baumlein H, Wobus U, Pustell J, Kafatos FC: The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element. Nucl Acids Res 14: 2707–2720 (1986).PubMedGoogle Scholar
  5. 5.
    Benoist C, Ohare K, Breathnach R, Chambon P: Ovalbumin gene-sequence of putative contral regions. Nucl Acids Res 8: 127–142 (1980).PubMedGoogle Scholar
  6. 6.
    Blin N, Staffod DW: A general method for isolation of high molecular weight DNA. Nucl Acids Res 3: 2303–2308 (1976).PubMedGoogle Scholar
  7. 7.
    Calos MP, Miller JH: Transposable elements. Cell 20: 579–595 (1980).CrossRefPubMedGoogle Scholar
  8. 8.
    Cho TJ, Davis CS, Nielsen NC: Inheritance and organization of genes in soybean. Plant Cell 1: 329–337 (1989).CrossRefPubMedGoogle Scholar
  9. 9.
    Devereux J, Haeberli P, Smithies O: A comprehensive test of sequence analysis for the VAX. Nucl Acids Res 12: 387–395 (1984).PubMedGoogle Scholar
  10. 10.
    Dickinson CD, Evans RP, Nielsen NC: RY-repeats are conserved in the 5′-flanking regions of legume seed-protein genes. Nucl Acids Res 16: 371 (1988).PubMedGoogle Scholar
  11. 11.
    Doyle JJ, Schuler MA, Godette WD, Zenger V, Beachy RN: The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris. J Biol Chem 216: 9288–9238 (1986).Google Scholar
  12. 12.
    Federoff N, Maurais J, Chaleff D: Molecular studies on mutations at the shruken locus in maize caused by the controlling elements Ds. J Mol Appl Genet 2: 11–29 (1983).PubMedGoogle Scholar
  13. 13.
    Fitzgerald M, Shenk T: The sequence 5′-AAUAAA-3′ forms part of the recognition site for polyadenylation of late SV40 mRNAs. Cell 24: 251–260 (1981).CrossRefPubMedGoogle Scholar
  14. 14.
    Henikoff S: Unidirectional digestion with exonuclease III creates targeted breakpoint for DNA sequencing. Gene 28: 351–359 (1984).CrossRefPubMedGoogle Scholar
  15. 15.
    Hong GF: A systematic DNA sequencing strategy. J Mol Biol 158: 539–549 (1982).PubMedGoogle Scholar
  16. 16.
    Iatron K, Tsitilou SG, Goldsmith MR, Kafatos FC: Molecular analysis of the GrB mutation in Bombyx mori through the use of a chorion cDNA library. Cell 20: 659–669 (1980).CrossRefPubMedGoogle Scholar
  17. 17.
    Lycett GW, Croy RRD, Shirsat AH, Boulter D: The complete nucleotide sequence of a legumin gene from pea (Pisum sativum L.). Nucl Acids Res 12: 4493–4506 (1984).PubMedGoogle Scholar
  18. 18.
    Lycett GW, Delauney AJ, Zhao W, Gatehouse JA, Croy RRD, Boulter D: Two cDNA clones coding for the legumin protein of Pisum sativum L. contain sequence repeats. Plant Mol Biol 3: 91–96 (1984).Google Scholar
  19. 19.
    Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).Google Scholar
  20. 20.
    Maxam AM, Gilbert W: Sequencing end-labeled DNA with base-specific chemical cleavages. Meth Enzymol 65: 499–560 (1980).PubMedGoogle Scholar
  21. 21.
    McClintock B: The control of gene action in maize. Brookhaven Symp Biol 18: 162–184 (1965).Google Scholar
  22. 22.
    Momma T, Negoro T, Hirano H, Matsumoto A, Udaka K, Fukazawa C: Glycinin A5A4B3: cDNA cloning and nucleotide sequencing of a splitting storage protein subunit of soybean. Eur J Biochem 149: 491–496 (1985).Google Scholar
  23. 23.
    Nielsen NC, Dickinson CD, Cho TJ, Thanh VH, Scallon BJ, Fisher RL, Sims TL, Drews GN, Goldberg RB: Characterization of the glycinin gene family in soybean. Plant Cell 1: 313–328 (1989).CrossRefPubMedGoogle Scholar
  24. 24.
    Pharmacia Biochemical: M13 Cloning/Sequencing System.Google Scholar
  25. 25.
    Saedler H, Bonas U, Daumling B, Gupta H, Hahlbrock K, Harrison BJ, Kreuzaler F, Peterson PA, Rief J, Schwarz-Sommer Z, Chepard N, Sommer H, Ubben D, Wienand U: Transposable elements in plants. In: Chater KF, Cullis CA, Hopwood DA, Johnston A, Woolhouse HW (eds) Genetic Rearrangements, pp. 107–115. Croom Helm Ltd., London (1983).Google Scholar
  26. 26.
    Sanger F, Nicklen S, Coulson AR: DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467 (1977).PubMedGoogle Scholar
  27. 27.
    Scallon BJ, Dickinson CD, Nielsen NC: Characterization of a null-allele for the Gy4 glycinin gene from soybean. Mol Gen Genet 208: 107–113 (1987).CrossRefGoogle Scholar
  28. 28.
    Scallon BJ, Thanh VH, Floener LA, Nielson NC: Identification and characterization of DNA clones encoding group-II glycinin subunits. Theor Appl Genet 70: 510–519 (1985).CrossRefGoogle Scholar
  29. 29.
    Shapiro JA (ed): Mobile Genetic Elements. Academic Press, New York (1983).Google Scholar
  30. 30.
    Simon AE, Tenbarge KM, Scofield SR, Finkelstein RR, Crouch ML: Nucleotide sequence of a cDNA clone of Brassica napus 12S storage protein shows homology with legumin from Pisum sativum. Plant Mol Biol 5: 191–201 (1985).Google Scholar
  31. 31.
    Southern EM: Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517 (1975).PubMedGoogle Scholar
  32. 32.
    Tumer NE, Thanh VH, Nielsen NC: Purification and characterization of mRNA from soybean seeds. J Biol Chem 256: 8756–8760 (1981).PubMedGoogle Scholar
  33. 33.
    Vodkin LO, Rhodess PR, Goldberg RB: cA lectin gene insertion has the structural features of a transposable element. Cell 34: 1023–1031 (1983).CrossRefPubMedGoogle Scholar
  34. 34.
    Xue ZT, Xu ML, Zhuang NL, Shen W, Shen SC: Cloning and characterization of two coding sequences for storage protein in soybean Glycine max. Scient Sin 29: 503–515 (1986).Google Scholar
  35. 35.
    Xue ZT, Xu ML, Zhuang NL, Shen W, Shen SC: Two expressed copies of glycinin Gy4 gene subfamily in wild soybean Glycine soja SH1. Scient Sin 30: 1289–1297 (1987).Google Scholar

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Zhong-Tian Xue
    • 1
  • Mei-Lin Xu
    • 1
  • Wei Shen
    • 1
  • Nai-Liang Zhuang
    • 1
  • Wei-Ming Hu
    • 1
  • San Chiun Shen
    • 1
  1. 1.Laboratory of Molecular Genetics, Shanghai Institute of Plant PhysiologyAcademia SinicaShanghaithe People's Republic of China
  2. 2.Department of Biochemistry, Chemical CentreUniversity of LundLundSweden
  3. 3.Department of Medical MicrobiologyUniversity of LundLundSweden

Personalised recommendations