Advertisement

Plant Molecular Biology

, Volume 14, Issue 2, pp 269–276 | Cite as

Improved binary vectors for Agrobacterium-mediated plant transformation

  • Kevin E. McBride
  • Kristin R. Summerfelt
Article

Abstract

Improved plant transformation vectors were constructed which utilize the pRiHRI origin of replication for highly stable maintenance in Agrobacterium tumefaciens, the ColE1 origin of replication for high copy maintenance in Escherichia coli, and a gentamycin resistance gene as a strong selectable marker for bacteria. Concise T-DNA elements were engineered with border sequences from the TL-DNA of pTiA6, the Tn5 neomycin phosphotransferase gene (npt II) expressed from either CaMV 35S or mannopine synthase (mas) promoters, and the lac Z′ gene segment from pUC18 as a source of unique restriction sites as well as an insertional inactivation marker for cloned DNA. The order of T-DNA components in all vectors is left border, plant marker cassette, lac Z′, and right border, respectively. The prototype vector, pCGN1547, was shown to be very stable in A. tumefaciens strain LBA4404 and to act as an efficient donor of T-DNA in tomato transformation experiments. Use of the other vectors is also described.

Key words

Agrobacterium binary vectors ColE1 replicon lac Z′ pRi replicon plant transformation 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    An G, Watson BD, Stachel S, Gordon MP, Nester EW: New cloning vehicles for transformation of higher plants. EMBO J 4: 277–284 (1985).Google Scholar
  2. 2.
    Barker RF, Idler KB, Thompson DV, Kemp JD: Nucleotide sequence of the T-DNA region from the Agrobacterium tumefaciens octopine Ti plasmid pTi15955. Plant Mol Biol 2: 335–350 (1983).Google Scholar
  3. 3.
    Bevan M: Binary Agrobacterium vectors for plant transformation. Nucl Acids Res 12: 8711–8721 (1984).PubMedGoogle Scholar
  4. 4.
    Buchanan-Wollaston V, Passiatore JE, Cannon F. The mob and ori T mobilization functions of a bacterial plasmid promote its transfer to plants. Nature (London) 328: 172–175 (1987).Google Scholar
  5. 5.
    Chilton MD, Currier TC, Farrand SK, Bendich AJ, Gordon MP, Nester EW: Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci USA 71: 3672–3676 (1974).PubMedGoogle Scholar
  6. 6.
    Dellaporta SJ, Wood J, Hicks JB: A plant DNA minipreparation: version II. Plant Mol Biol Rep 1: 19–21 (1983).Google Scholar
  7. 7.
    Fillatti JJ, Kiser J, Rose R, Comai L: Efficient transfer of a glyphosate tolerance gene into tomato using a binary Agrobacterium tumefaciens vector. Bio/Technology 5: 726–730 (1987).CrossRefGoogle Scholar
  8. 8.
    Gardner RC, Chonoles KR, Owens RA: Potato spindle tuber viroid infections mediated by the Ti plasmid of Agrobacterium tumefaciens. Plant Mol Biol 6: 221–228 (1986).Google Scholar
  9. 9.
    Hirsch PR, Beringer JE: A physical map of pPH1JI and pJB4JI. Plasmid 12: 139–141 (1984).PubMedGoogle Scholar
  10. 10.
    Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA: A binary vector strategy based on separation of vir and T-region of the Agrobacterium tumefaciens Ti plasmid. Nature (London) 303: 179–181 (1983).Google Scholar
  11. 11.
    Holsters M, deWarle D, Depicker A, Messens E, VanMontague M, Schell J: Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet 163: 181–187 (1979).CrossRefGoogle Scholar
  12. 12.
    Houck CM, Shintani DK, Knauf VC: Agrobacterium as a gene transfer agent for plants. Frontiers Appl Microbiol 4 (1988).Google Scholar
  13. 13.
    Ish-Horowicz D, Burke JF: Rapid and efficient cosmid cloning. Nucl Acids Res 9: 2989–2998 (1981).PubMedGoogle Scholar
  14. 14.
    Jouanin L, Vilaine F, d'Enfert C, Casse-Delbart F: Localization and restriction maps of the replication origin regions of the plasmids of Agrobacterium rhizogenes strain A4. Mol Gene Genet 201: 370–374 (1985).CrossRefGoogle Scholar
  15. 15.
    Mandel M, Higa A: Calcium dependent bacteriophage DNA infection. J Mol Biol 53: 154–162 (1970).Google Scholar
  16. 16.
    Maniatis T, Fritsch EF, Sambrook J: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1982).Google Scholar
  17. 17.
    Nishiguchi R, Takanami M, Oka A: Characterization and sequence determination of the replicator region in the hairy-root-inducing plasmid pRiA4b. Mol Gen Genet 206: 1–8 (1987).CrossRefGoogle Scholar
  18. 18.
    Ooms G, Hooykaas PJJ, VanVeen RJM, VanBeelen P, Regensburg-Tuink TJG, Schilperoort RA: Octopine Tiplasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region. Plasmid 7: 15–29 (1982).PubMedGoogle Scholar
  19. 19.
    Peralta EG, Hellmiss R, Ream W: Overdrive, a T-DNA transmission enhancer on the A. tumefaciens tumor inducing plasmid. EMBO J 5: 1137–1142 (1986).Google Scholar
  20. 20.
    Schmidhauser TJ, Helinski DR. Regions of broad-hostrange plasmid pRK2 involved in replication and stable maintenance in nine species on gram-negative bacteria. J Bacteriol 164: 446–455 (1985).PubMedGoogle Scholar
  21. 21.
    Sutcliffe JG: Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harbor Symp Quant Biol 43: 77–90 (1979).PubMedGoogle Scholar
  22. 22.
    Vieira J, Messing J: The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing using universal primers. Gene 19: 259–268 (1982).CrossRefPubMedGoogle Scholar
  23. 23.
    Wang K, Herrera-Estrella L, VanMontague M, Zambryski P: Right 25 bp terminus sequence of the nopaline T-DNA is essential for and determines direction of DNA transfer from Agrobacterium to the plant genome. Cell 38: 455–462 (1984).CrossRefPubMedGoogle Scholar
  24. 24.
    Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: nucleotide sequence of M13mp18 and vectors. Gene 33: 103–119 (1985).CrossRefPubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Kevin E. McBride
    • 1
  • Kristin R. Summerfelt
    • 1
  1. 1.Calgene Inc.DavisUSA

Personalised recommendations