Plant Molecular Biology

, Volume 14, Issue 2, pp 119–126

Tobacco genes encoding acidic and basic isoforms of pathogenesis-related proteins display different expression patterns

  • Johan Memelink
  • Huub J. M. Linthorst
  • Rob A. Schilperoort
  • J. Harry C. Hoge
Article

DOI: 10.1007/BF00018553

Cite this article as:
Memelink, J., Linthorst, H.J.M., Schilperoort, R.A. et al. Plant Mol Biol (1990) 14: 119. doi:10.1007/BF00018553

Abstract

The induction by cytokinin stress and ethylene of nine different tobacco mosaic virus-inducible mRNA classes (termed A-I) encoding pathogenesis-related (PR) proteins was studied. The induced mRNA levels were compared to basal levels in healthy tobacco plants grown in tissue culture and in a greenhouse. Cytokinin stress and ethylene were found to induce different subsets of the mRNAs, indicating that ethylene is not the primary inducing signal in cytokinin-stressed shoots. mRNAs F, H and G encoding the basic hydrolytic enzymes chitinase, β-1,3-glucanase and a basic equivalent of PR-1, respectively, were found to be expressed at high levels in roots of healthy plants. mRNAs D, I and B encoding the acidic equivalents of the proteins proved to be present at low levels in healthy plants. These results indicate that genes encoding basic and acidic isoforms of pathogenesis-related proteins are differentially regulated.

Key words

β-1,3-glucanase mRNA chitinase mRNA cytokinin ethylene pathogenesis-related mRNAs tobacco 

Copyright information

© Kluwer Academic Publishers 1990

Authors and Affiliations

  • Johan Memelink
    • 1
  • Huub J. M. Linthorst
    • 2
  • Rob A. Schilperoort
    • 1
  • J. Harry C. Hoge
    • 1
  1. 1.Molbas Research Group, Department of Plant Molecular BiologyLeiden UniversityLeidenNetherlands
  2. 2.Department of Biochemistry, Leiden UniversityGorlaeus LaboratoriesLeidenNetherlands

Personalised recommendations