Plant Molecular Biology

, Volume 18, Issue 1, pp 83–95

Structural organization of the chloroplast genome of the chromophytic algaVaucheria bursata

  • Karl-Heinz Linne von Berg
  • Klaus V. Kowallik
Article

DOI: 10.1007/BF00018459

Cite this article as:
Linne von Berg, KH. & Kowallik, K.V. Plant Mol Biol (1992) 18: 83. doi:10.1007/BF00018459

Abstract

The chloroplast genome of the chromophytic algaVaucheria bursata has been characterized by restriction site and gene mapping analysis. It is represented by a circular molecule 124.6 kb in size. An inverted sequence duplication (IR) not larger than 5.85 kb carries the rRNA genes and separates two single-copy regions of 64.6 kb and 48.3 kb from one another. TheVaucheria plastid genome exists in two equimolar isomers which is due to intramolecular flip-flop recombination within the IR sequences. The coding sites for 21 structural and soluble proteins have been mapped on both single-copy regions using heterologous gene sequences as probes. Although the overall gene order is found to be rearranged when compared with other chromophytic algal and land plant chloroplast genomes, most of the transcriptional units of cyanobacteria and land plant chloroplast genomes appear to be conserved. The phylogenetic implications of these findings are further discussed.

Key words

chloroplast genome gene mapping inverted repeat flip-flop recombination Chromophyta Vaucheria bursata 

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Karl-Heinz Linne von Berg
    • 1
  • Klaus V. Kowallik
    • 1
  1. 1.Institut für Botanik der Heinrich-Heine-UniversitätUniversitätsstrasse 1DüsseldorfFRG
  2. 2.Botanisches Institut der Universität KölnKöln 41FRG

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