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Photosynthesis Research

, Volume 11, Issue 2, pp 109–118 | Cite as

Observation and characterisation of a transient in the yield of chlorophyll fluorescence in intact spinach chloroplasts

  • K. A. Carver
  • P. Horton
Regular Paper

Abstract

A transient in chlorophyll fluorescence, which is associated with a transient in 9-aminoacridine fluorescence and a perturbation in the rate of oxygen evolution, has been observed in intact spinach chloroplasts. The results indicate that changes in the redox state of Q are, at least partially, responsible for the transient in chlorophyll fluorescence. The size of the transient is highly dependent upon the concentration of inorganic phosphate and upon the pH of the medium. The properties of the transient are consistent with the suggestion that it reflects changes in the levels of stromal intermediates during induction.

Key words

photosynthesis chloroplast chlorophyll fluorescence 

Abbreviations

BES

NN-Bis(2-hydroxyethyl)2-aminoethanesulphonic acid dihydroxyacetone-P(DHAP): dihydroxyacetone phosphate glycerate-3-P (PGA): glycerate-3-phosphate

HEPES

N-2-Hydroxyethylpiperazine-N-2-ethanesulphonic acid

MES

2-(N-Morpholino)ethanesulphonic acid

Pi

inorganic phosphate

qE

quenching of chlorophyll fluorescence by the energisation of the thylakoid membrane

qQ

quenching of chlorophyll fluorescence by oxidised Q, the electron acceptor of photosystem 2

ribose-5-P (R5P)

ribose-5-phosphate

Rbu-5-P

ribulose-5-phosphate

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Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • K. A. Carver
    • 1
  • P. Horton
    • 1
    • 2
  1. 1.Research Institute for PhotosynthesisThe UniversitySheffieldUK
  2. 2.Department of BiochemistryThe UniversitySheffieldUK

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