Photosynthesis Research

, Volume 11, Issue 2, pp 109–118 | Cite as

Observation and characterisation of a transient in the yield of chlorophyll fluorescence in intact spinach chloroplasts

  • K. A. Carver
  • P. Horton
Regular Paper


A transient in chlorophyll fluorescence, which is associated with a transient in 9-aminoacridine fluorescence and a perturbation in the rate of oxygen evolution, has been observed in intact spinach chloroplasts. The results indicate that changes in the redox state of Q are, at least partially, responsible for the transient in chlorophyll fluorescence. The size of the transient is highly dependent upon the concentration of inorganic phosphate and upon the pH of the medium. The properties of the transient are consistent with the suggestion that it reflects changes in the levels of stromal intermediates during induction.

Key words

photosynthesis chloroplast chlorophyll fluorescence 



NN-Bis(2-hydroxyethyl)2-aminoethanesulphonic acid dihydroxyacetone-P(DHAP): dihydroxyacetone phosphate glycerate-3-P (PGA): glycerate-3-phosphate


N-2-Hydroxyethylpiperazine-N-2-ethanesulphonic acid


2-(N-Morpholino)ethanesulphonic acid


inorganic phosphate


quenching of chlorophyll fluorescence by the energisation of the thylakoid membrane


quenching of chlorophyll fluorescence by oxidised Q, the electron acceptor of photosystem 2

ribose-5-P (R5P)





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  1. 1.
    Carver KA, Hope AB and Walker DA (1983) Adenine nucleotide status, phosphoglycerate reduction and photosynthetic phosphorylation in a reconstituted chloroplast system. Biochem J 210: 273–276Google Scholar
  2. 2.
    Cerovic ZG, Sivak MN and Walker DA (1984) Slow secondary fluorescence kinetics associated with the onset of photosynthetic carbon assimilation in intact isolated chloroplasts. Proc Roy Soc Lond. B 220: 327–338Google Scholar
  3. 3.
    Horton P (1983) Relations between electron transport and carbon assimilation simultaneous measurement of chlorophyll fluorescence, transthylakoid pH gradient and O2 evolution in isolated chloroplasts. Proc Roy Soc Lond B 217: 405–416.Google Scholar
  4. 4.
    Horton P (1985a) Regulation of photochemistry and its interaction with carbon metabolism. In: Jeffcoat B, Hawkins AF, Stead AD (eds) Regulation of source and sinks in crop plants. British Plant Growth Regulator Group, Bristol, pp 19–33.Google Scholar
  5. 5.
    Horton P (1985b) Interactions between electron transfer and carbon assimilation. In: Barber J, Baker NR (eds) Photosynthetic mechanisms and the environment. Elsevier Science Pubs, BV, pp 135–187.Google Scholar
  6. 6.
    Horton P, Lee P and Anderson S (1984) Fluorescence induction in a thylakoid system reconstituted for photosynthetic carbon assimilation. In: (Sybesma C ed) Advances in photosynthesis research Martinus Nijhoff/Dr W Junk Pubs, The Hague, Vol III, pp 657–660Google Scholar
  7. 7.
    Krause GH, Vernotte C and Briantais JM (1982) Photoreduced quenching of chlorophyll fluorescence in intact chloroplasts and algae, resolution into two components. Biochim Biophys Acta 679: 116–124.Google Scholar
  8. 8.
    Lilley RMcC and Walker DA (1974) The reduction of 3-phosphoglycerate by reconstituted chloroplasts and by chloroplast extracts. Biochim Biophys Acta 368: 269–278.Google Scholar
  9. 9.
    Nakamoto H, Sivak MN and Walker DA (1987) Sudden changes in the rate of photosynthetic oxygen evolution and chlorophyll fluorescence in intact isolated chloroplasts: the role of orthophosphate. Photosynth Res 11: 119–130.Google Scholar
  10. 10.
    Quick P and Horton P (1984a) Studies on the induction of chlorophyll fluorescence in barley protoplasts. I. Factors affecting the observation of oscillations in the yield of chlorophyll fluorescence and the rate of oxygen evolution. Proc Roy Soc Lond B 220: 361–370.Google Scholar
  11. 11.
    Quick P and Horton P (1984b) Studies on the induction of chlorophyll fluorescence in barley protoplasts. II. Resolution of fluorescence quenching by redox state and the transthylakoid pH gradient. Proc Roy Soc Lond B 220: 271–382.Google Scholar
  12. 12.
    Quick P and Horton P (1986) Studies on the induction of chlorophyll fluorescence in barley protoplasts. III. Correlation between changes in the level of glycerate 3-phosphate and the pattern of fluorescence quenching. Biochim Biophys Acta, 849: 1–6.Google Scholar
  13. 13.
    Robinson SP and Walker DA (1979) The control of 3-phosphoglycerate reduction in isolated chloroplasts by the concentrations of ATP, ADP and 3-phosphoglycerate. Biochim Biophys Acta 545: 528–536Google Scholar
  14. 14.
    Sivak MN and Walker DA (1983) Some effects of CO2 concentration and decreased O2 concentration on induction fluorescence in leaves. Proc Roy Soc Lond B 217: 377–392.Google Scholar
  15. 15.
    Sivak MN and Walker DA (1985) Chlorophyll a fluorescence; can it shed light on fundamental questions in photosynthetic carbon dioxide fixation? Plant Cell and Environment 8: 439–448Google Scholar
  16. 16.
    Sivak MN, Dietz K-J, Heber U and Walker DA (1985) The relationship between light-scattering and chlorophyll a fluorescence during oscillations in photosynthetic carbon assimilation. Arch Biochem Biophys 237: 513–519Google Scholar
  17. 17.
    Walker DA (1980) Preparation of higher plant chloroplasts. In: (San Pietro A ed) Methods in Enzymology, Academic Press, New York, Vol 69, pp 94–104Google Scholar
  18. 18.
    Walker DA (1981a) Photosynthetic induction. In: (Akoyonoglou G ed) Proc 5th Int Cong Photosynthesis, Kassandra-Halkidiki, Balaban Int Sci Services, Philadelphia Vol IV, pp 189–202Google Scholar
  19. 19.
    Walker DA (1981b) Secondary fluorescence kinetics of spinach leaves in relation to the onset of photosynthetic carbon assimilation. Planta 153: 273–278Google Scholar
  20. 20.
    Walker DA and Sivak MN (1985) Can phosphate limit photosynthetic carbon assimilation in vivo? Physiologie Veg. Special Issue 23: 829–841Google Scholar
  21. 21.
    Walker DA, Horton P, Sivak MN and Quick WP (1983) Anti-parallel relationship between O2 evolution and slow fluorescence induction kinetics. Photobiochem and Photobiophys 5: 35–39Google Scholar
  22. 22.
    Walker DA, Sivak MN, Prinsley RT and Cheesbrough JK (1983) Simultaneous measurement of oscillations in oxygen evolution and chlorophyll a fluorescence in leaf pieces. Plant Physiol 73: 542–549Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1987

Authors and Affiliations

  • K. A. Carver
    • 1
  • P. Horton
    • 1
    • 2
  1. 1.Research Institute for PhotosynthesisThe UniversitySheffieldUK
  2. 2.Department of BiochemistryThe UniversitySheffieldUK

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