Plant and Soil

, Volume 39, Issue 1, pp 205–207 | Cite as

Rapid determination of free proline for water-stress studies

  • L. S. Bates
  • R. P. Waldren
  • I. D. Teare
Short Communication

Summary

Proline, which increases proportionately faster than other amino acids in plants under water stress, has been suggested as an evaluating parameter for irrigation scheduling and for selecting drought-resistant varieties. The necessity to analyze numerous samples from multiple replications of field grown materials prompted the development of a simple, rapid colorimetric determination of proline. The method detected proline in the 0.1 to 36.0 μmoles/g range of fresh weight leaf material.

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References

  1. 1.
    Barnett, N. M. and Naylor, A. W., Plant Physiol. 41, 1222 (1966).Google Scholar
  2. 2.
    Chinard, E. P., J. Biol. Chem. 199, 91 (1952).PubMedGoogle Scholar
  3. 3.
    Kimura, Y., Anal. Chim. Acta. 37, 240 (1967).CrossRefPubMedGoogle Scholar
  4. 4.
    Messer, M., Anal. Biochem. 2, 353 (1961).PubMedGoogle Scholar
  5. 5.
    Palfi, G. and Juhasz, J., Plant and Soil 34, 503 (1971).Google Scholar
  6. 6.
    Thompson, J. F., et al., Plant Physiol. 41, 1578 (1966).Google Scholar
  7. 7.
    Troll, W., and Lindsley, J., J. Biol. Chem. 215, 655 (1955).PubMedGoogle Scholar
  8. 8.
    Wren, J. J. and Wiggall, P. H., Biochem. J. 94 216 (1965)PubMedGoogle Scholar

Copyright information

© Martinus Nijhoff Publishers 1973

Authors and Affiliations

  • L. S. Bates
    • 1
  • R. P. Waldren
    • 2
  • I. D. Teare
    • 2
  1. 1.Department of Grain Science and IndustryKansas State UniversityManhattanUSA
  2. 2.Evapotranspiration LaboratoryKansas State UniversityManhattanUSA

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