Plant and Soil

, Volume 149, Issue 2, pp 205–209 | Cite as

Near isogenic lines of soybeans as tools to identify nodulation specificmutants of Bradyrhizobium elkanii

  • T. E. Devine
  • J. J. O'Neill
  • L. D. Kuykendall
Research Article


The dominant allele Rj4 in soybean interdicts or restricts the nodulation of plants by certain strains of bacteria, most of which are classified as Bradyrhizobium elkanii, while the recessive allele permits normal nodulation with the same strains. The near isogenic lines BARC-2 (Rj4) and BARC-3 (rj4) are calculated to be 99.95% identical in their nuclear DNA, but differ specifically in the allele present at the Rj4 locus. These lines were used to identify spontaneous mutants of the Rj4-restricted Bradyrhizobium elkanii strain USDA 61 Nalr that had the ability to effectively nodulate plants of the Rj4 genotype. Of the eight rare nodules found on roots of soybean plants of the Rj4 genotype inoculated with the genetically marked strain USDA 61 Nalr, four were identified as containing mutants with the ability to overcome the effects of the Rj4 allele.

Key words

genetics ineffective nodulation mutants nitrogen fixation symbiosis Rj4 gene 


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Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • T. E. Devine
    • 1
  • J. J. O'Neill
    • 1
  • L. D. Kuykendall
    • 2
  1. 1.Plant Molecular Biology LaboratoryUSDA, ARS, Beltsville Agricultural Research CenterBeltsvilleUSA
  2. 2.Soybean and Alfalfa Research LaboratoryUSDA, ARS, Beltsville Agricultural Research CenterBeltsvilleUSA

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