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Plant Molecular Biology

, Volume 5, Issue 3, pp 137–147 | Cite as

Isolation and characterisation of cDNA clones for tomato polygalacturonase and other ripening-related proteins

  • Adrian Slater
  • Martin Jack Maunders
  • Keith Edwards
  • Wolfgang Schuch
  • Donald Grierson
Article

Summary

Gene expression during the ripening of tomato fruit was investigated by cDNA cloning and hybrid-select translation. A cDNA library was prepared from poly(A)-containing mRNA from ripe tomato fruit and sreened by differential hybridization. 146 ripening-related cDNA clones were found. Eleven groups and eight unique clones have been identified so far. The sizes of the cloned cDNA inserts were determined and type-members for seven groups were used in hybrid selection experiments. Six of the seven clones encode translation products corresponding to six ripening related polypeptides detected previously by in vitro translation of total cytoplasmic RNA (14). One cDNA group codes for a Mr 48 000 protein that was identified as polygalacturonase on the basis of immunoprecipitation with specific antiserum raised against tomato polygalacturonase. re]19840918 rv]19850613 ac]19850618

Keywords

cDNA hybrid-select translation mRNA polygalacturonase tomato ripening 

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Copyright information

© Martinus Nijhoff Publishers 1985

Authors and Affiliations

  • Adrian Slater
    • 1
  • Martin Jack Maunders
    • 1
  • Keith Edwards
    • 2
  • Wolfgang Schuch
    • 2
  • Donald Grierson
    • 1
  1. 1.Department of Physiology and Environmental ScienceUniversity of Nottingham Faculty of Agricultural ScienceLoughboroughU.K.
  2. 2.ICI Corporate Bioscience GroupRuncornU.K.

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