Plant Molecular Biology

, Volume 25, Issue 6, pp 989–994 | Cite as

The small, versatilepPZP family ofAgrobacterium binary vectors for plant transformation

  • Peter Hajdukiewicz
  • Zora Svab
  • Pal Maliga
Research Article


The newpPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322bom site for mobilization fromEscherichia coli toAgrobacterium, and the ColE1 and pVS1 plasmid origins for replication inE. coli and inAgrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use inAgrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kanamycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ α-peptide, with the pUC18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants.

Key words

Agrobacterium binary vectors gentamycin resistance kanamycin resistance tobacco 


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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Peter Hajdukiewicz
    • 1
  • Zora Svab
    • 1
  • Pal Maliga
    • 1
  1. 1.Waksman Institute, RutgersThe State University of New JerseyPiscatawayUSA

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