Plant Molecular Biology

, Volume 21, Issue 4, pp 705–708

Transgenic tobacco plants regenerated from leaf disks can be periclinal chimeras

  • T. Schmülling
  • Jeff Schell
Update Section Short Communication

Abstract

Amongst rolC transgenic tobacco plants regenerated from leaf disks 6.5% are periclinal chimeras, i.e. plants with genetically different cell populations in different cell layers. The expression of the rolC gene of Agrobacterium rhizogenes causes a reduction in pigment content in leaves. The chimeric composition of the regenerated plants becomes thus apparent as light green leaf tissue in the transgenic region, tissue flanked by dark green wild-type sectors. Southern and northern blot analysis confirmed the chimeric nature of such plants. Investigation of selfed progeny of chimeric plants on selective media indicates that layer invasion in reproductive tissues can occur in tobacco early during the formation of the flower buds. The results show (1) that tobacco plants regenerated from leaf disks and grown on selective media have not necessarily the same clonal origin and (2) that they can give rise to non-transgenic offspring. The chimeric plants provide insight on the effect of rolC gene expression on microsporogenesis.

Key words

chimera transgenic plant Agrobacterium rhizogenes rolC gene 

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References

  1. 1.
    Attfield EM, Evans PK: Developmental pattern of root and shoot organogenesis in altered leaf explants of Nicotiana tabacum cv. Xanthi nc. J Exp Bot 42: 51–57 (1991).Google Scholar
  2. 2.
    Dawe RK, Freeling M: Clonal analysis of the cell lineages in the male flower of maize. Devel Biol 142: 233–245 (1990).Google Scholar
  3. 3.
    Firoozabady E: Rapid plant regeneration from Nicotiana mesophyll protoplasts. Plant Science 46: 127–131 (1986).CrossRefGoogle Scholar
  4. 4.
    Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SD, Fraley RT: A simple and general method for transferring genes into plants. Science 227: 1229–1231 (1985).Google Scholar
  5. 5.
    Irish VF: Cell lineage in plant development. Curr Opin Genet Devel 1: 169–173 (1991).Google Scholar
  6. 6.
    Maliga P, Sz.-Breznovitis A, Marton L: Streptomycinresistant plants from callus culture from haploid tobacco. Nature 347: 737–741 (1973).Google Scholar
  7. 7.
    Murashige T, Skoog F: A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol Plant 15: 473–496 (1962).Google Scholar
  8. 8.
    Poethig S: Genetic mosaics and cell lineage analysis in plants. Trends Genet 5: 273–277 (1989).CrossRefPubMedGoogle Scholar
  9. 9.
    Schmülling T: Studien zum Einfluβ der rolA, B und C Gene der TL-DNA von Agrobacterium rhizogenes auf die Pflanzenentwicklung. Dissertation, Universität zu Köln (1988).Google Scholar
  10. 10.
    Schmülling T, Schell J, Spena A: Single genes from Agrobacterium rhizogenes influence plant development. EMBO J 7: 2621–2629 (1988).Google Scholar
  11. 11.
    Schmülling T, Röhrig H, Pilz S, Walden R, Schell J: Restoration of fertility by antisense RNA in genetically engineered male sterile tobacco plants. Mol Gen Genet, in press.Google Scholar
  12. 12.
    Spena A, Aalen RB, Schulze S: Cell-autonomous behavior of the rolC gene of Agrobacterium rhizogenes during leaf development: Visual assay for transposon excision in transgenic plants. Plant Cell 1: 1157–1164 (1989).CrossRefPubMedGoogle Scholar
  13. 13.
    Stewart RN: Ontogeny of the primary body in chimeral forms of higher plants. In: Subtelny S, Sussex IM (eds) The clonal basis of development. Academic Press, New York, 131–160 (1978).Google Scholar
  14. 14.
    Tilney-Bassett RAE: Plant chimeras. Arnold, London (1986).Google Scholar

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • T. Schmülling
    • 1
  • Jeff Schell
    • 2
  1. 1.Universität Tübingen, Lehrstuhl für Allgemeine GenetikTübingenGermany
  2. 2.Max-Planck-Institut für ZüchtungsforschungKöln 30Germany

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