Differential expression of two related, low-temperature-induced genes in Arabidopsis thaliana (L.) Heynh
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Plant cold acclimation is correlated to expression of low-temperature-induced (lti) genes. By using a previously characterized lti cDNA clone as a probe we isolated a genomic fragment that carried two closely located lti genes of Arabidopsis thaliana. The genes were structurally related with the coding regions interrupted by three similarly located short introns and were transcribed in the same direction. The nucleotide sequences of the two genes, lti78 and lti65, predict novel hydrophilic polypeptides with molecular weights of 77856 and 64510, respectively, lti78 corresponding to the cDNA probe. Of the 710 amino acids of LTI78 and 600 amino acids of LTI65, 346 amino acids were identical between the polypeptides, which suggests that the genes may have a common origin.
Both lti78 and lti65 were induced by low temperature, exogenous abscisic acid (ABA) and drought, but the responsiveness of the genes to these stimuli was markedly different. Both the levels and the temporal pattern of expression differed between the genes. Expression of lti78 was mainly responsive to low temperature, that of lti65 to drought and ABA. In contrast to the induction of lti78, which follows separate signal pathways during low-temperature, ABA and drought treatment, the drought induction of lti65 is ABA-dependent and the low-temperature induction appears to be coupled to the ABA biosynthetic pathway. This differential expression of two related genes may indicate that they have some-what different roles in the stress response.
Key wordsabscisic acid Arabidopsis thaliana cold acclimation drought stress signal transduction stress response
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- 1.Bray E: Drought- and ABA-induced changes in polypeptide and mRNA accumulation in tomato leaves. Plant Physiol 88: 1210–1214 (1988).Google Scholar
- 2.Cattivelli L, Bartels D: Molecular cloning and characterization of cold-regulated genes in barley. Plant Physiol 93: 1504–1510 (1990).Google Scholar
- 3.Chen THH, Gusta LV: Abscisic acid-induced freezing resistance in cultured plant cells. Plant Physiol 73: 71–75 (1983).Google Scholar
- 4.Cloutier Y, Siminovitch D: Correlation between cold- and drought-induced frost hardiness in winter wheat and rye varieties. Plant Physiol 69: 256–258 (1982).Google Scholar
- 5.DureIII L, Crouch M, Harada J, Ho THD, Mundy J, Quatrano R, Thomas T, Sung ZR: Common amino acid sequence domains among LEA proteins of higher plants. Plant Mol Biol 12: 475–486 (1989).Google Scholar
- 7.Gilmour SJ, Hajela RK, Thomashow MF: Cold acclimation in Arabidopsis thaliana. Plant Physiol 87: 745–750 (1988).Google Scholar
- 10.Guy CL: Cold acclimation and freezing stress tolerance: role of protein metabolism. Annu Rev Plant Physiol Plant Mol Biol 41: 187–223 (1990).Google Scholar
- 11.Hajela RK, Horvath DP, Gilmour SJ, Thomashow MJ: Molecular cloning and expression of cor (cold-regulated) genes in Arabidopsis thaliana. Plant Physiol 93: 1246–1252 (1990).Google Scholar
- 12.Koornneef M, Jorna ML, Brinkhorst-van der Swan DLC, Karssen CM: The isolation of abscisic acid (ABA) deficient mutants by selection of induced revertants in non-germinating gibberellin sensitive lines of Arabidopsis thaliana (L.) Heynh. Theor Appl Genet 61: 385–393 (1982).Google Scholar
- 13.Koornneef M, Reuling G, Karssen CM: The isolation and characterization of abscisic acid-insensitive mutants of Arabidopsis thaliana. Physiol Plant 61: 377–383 (1984).Google Scholar
- 14.Kurkela S: Structure and Expression Analysis of an Arabidopsis thaliana Gene Family of Two Cold Induced Genes. Doctoral dissertation, University of Helsinki, Yliopistopaino, Helsinki (1991).Google Scholar
- 15.Kurkela S, Borg-Franck M: Structure and expression of kin2, one of two cold- and ABA-induced genes of Arabidopsis thaliana. Plant Mol Biol 9: 689–692 (1992).Google Scholar
- 18.Levitt J: Response of Plants to Environmental Stresses, volume 1, 2nd ed. Academic Press, New York (1980).Google Scholar
- 19.Lin C, Guo WW, Everson E, Thomashow MF: Cold acclimation in Arabidopsis and wheat. Plant Physiol 94: 1078–1083 (1990).Google Scholar
- 23.Mohapatra SS, Poole RJ, Dhindsa RS: Abscisic acid-regulated gene expression in relation to freezing tolerance in alfalfa. Plant Physiol 87: 468–473 (1988).Google Scholar
- 27.Orr W, Betty I, White TC, Robert LS, Singh J: Complementary DNA sequence of a low temperature-induced Brassica napus gene with a homology to the Arabidopsis thaliana kin1 gene. Plant Physiol 98: 1532–1534 (1992).Google Scholar
- 31.Sakai A, Larcher W: Frost Survival of Plants. Responses and Adaptation to Freezing Stress. Springer-Verlag, Berlin/Heidelberg/New York (1987).Google Scholar
- 35.Thomashow MF: Molecular genetics of cold acclimation in higher plants. Adv Genet 28: 99–131 (1990).Google Scholar