Plant Molecular Biology

, Volume 20, Issue 2, pp 333–336 | Cite as

Molecular cloning of the gene encoding developing seed l-asparaginase from Lupinus angustifolius

  • James M. J. J. Dickson
  • Eva Vincze
  • Murray R. Grant
  • Laura A. Smith
  • Karen A. Rodber
  • Kevin J. F. Farnden
  • Paul H. S. Reynolds
Update Section Short Communication

Abstract

A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5′ flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5′ of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5′ flanking region contained sequences associated with seed-specific expression.

Key words

L-asparaginase DNA sequence developing seed Lupinus angustifolius (lupin) 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • James M. J. J. Dickson
    • 1
  • Eva Vincze
    • 1
  • Murray R. Grant
    • 1
  • Laura A. Smith
    • 1
  • Karen A. Rodber
    • 1
  • Kevin J. F. Farnden
    • 2
  • Paul H. S. Reynolds
    • 1
  1. 1.Immunology and Plant Molecular Biology UnitDepartment of Scientific and Industrial Research-Fruit and TreesPalmerston NorthNew Zealand
  2. 2.Biochemistry DepartmentOtago UniversityDunedinNew Zealand

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