Plant Molecular Biology

, Volume 20, Issue 2, pp 289–299

Import and processing of the precursor of the Rieske FeS protein of tobacco chloroplasts

  • F. Madueño
  • J. A. Napier
  • F. J. Cejudo
  • J. C. Gray
Research Article

Abstract

cDNA clones encoding the precursor of the Rieske FeS protein of tobacco chloroplasts have been characterised and shown to derive from two different genes. The 5′ ends of the corresponding transcripts have been cloned using primer extension and PCR. The nucleotide sequences of the cDNAs (and their 5′ extensions) predict precursors for the tobacco proteins which differ in 4 amino acid residues out of a total of 228 residues and show high homology with the pea and spinach precursors. The tobacco precursor proteins contain N-terminal presequences of 49 amino acid residues which lack 17 amino acid residues present at the N-terminus of the spinach presequence. The 26 kDa precursor obtained by transcription and translation of one of these cDNAs in vitro was efficiently imported and correctly processed to the mature 20 kDa protein by isolated pea or tobacco chloroplasts. The precursor was also processed to its mature size by a peptidase present in the stroma of chloroplasts.

Key words

chloroplast cytochrome bf complex presequence Rieske FeS protein stromal processing peptidase 

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Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • F. Madueño
    • 1
    • 2
  • J. A. Napier
    • 1
    • 2
  • F. J. Cejudo
    • 3
  • J. C. Gray
    • 1
    • 2
  1. 1.Department of Plant SciencesUniversity of CambridgeCambridgeUK
  2. 2.Cambridge Centre for Molecular RecognitionUniversity of CambridgeCambridgeUK
  3. 3.The Sainsbury LaboratoryJohn Innes CentreNorwichUK
  4. 4.Long Ashton Research StationAFRC Institute of Arable Crops ResearchBristolUK

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