Plant Molecular Biology

, Volume 26, Issue 2, pp 763–769 | Cite as

Primary structure of the photosynthetic pyruvate orthophosphate dikinase of the C3 plant Flaveria pringlei and expression analysis of pyruvate orthophosphate dikinase sequences in C3, C3–C4 and C4Flaveria species

  • Elke Rosche
  • Monika Streubel
  • Peter Westhoff
Short Communication

Abstract

We have isolated full-size cDNA sequences encoding the photosynthetic isoform of pyruvate orthophosphate dikinase (PPDK) of the C3 plant Flaveria pringlei. The encoded protein shares 96% identical amino acid residues with the C4 isoform of PPDK in the C4 species F. trinervia. The differing amino acid residues are evenly distributed along the polypeptide chain. Genomic Southern analysis of photosynthetic PPDK sequences in F. pringlei (C3), F. chloraefolia (C3–C4), F. linearis (C3–C4), F. floridana (C3–C4), F. brownii (C4-like) and F. trinervia (C4) reveals a simple hybridization pattern which is suggestive of a single gene. Northern hybridization experiments show that the abundance of PPDK transcripts in leaves correlates with the degree of C4 characteristics expressed in the various photosynthetic types analysed. This finding demonstrates that the increase in expression levels must have played a crucial role in evolving the C4-PPDK gene in the genus Flaveria.

Key words

C4 photosynthesis molecular evolution pyruvate orthophosphate dikinase Flaveria 

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Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Elke Rosche
    • 1
  • Monika Streubel
    • 1
  • Peter Westhoff
    • 1
  1. 1.Institut für Entwicklungs- und Molekularbiologie der PflanzenHeinrich-Heine-Universität DüsseldorfDüsseldorfGermany

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