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Plant and Soil

, Volume 130, Issue 1–2, pp 231–241 | Cite as

Siderophores of Pseudomonas putida as an iron source for dicot and monocot plants

  • E. Bar-Ness
  • Y. Chen
  • Y. Hadar
  • H. Marschner
  • V. Römheld
Article

Abstract

Iron uptake from ferrated (59Fe) pseudobactin (PSB), a Pseudomonas putida siderophore, by various plant species was studied in nutrient solution culture under short term (10 h) and long term (3 weeks) conditions. In the short term experiments, 59Fe uptake rate from 59FePSB by dicots (peanuts, cotton and sunflower) was relatively low when compared with 59Fe uptake rate from 59FeEDDHA. Iron uptake rate from 59FePSB was pH and concentration dependent, as was the Fe uptake rate from 59FeEDDHA. The rate was about 10 times lower than that of Fe uptake from the synthetic chelate. Results were similar for long term experiments.

Monocots (sorghum) in short term experiments exhibited significantly higher uptake rate of Fe from FePSB than from FeEDDHA. In long term experiments, FePSB was less efficient than FeEDDHA as an Fe source for sorghum at pH 6, but the same levels of leaf chlorophyll concentration were obtained at pH 7.3.

Fe uptake rates by dicots from the siderophore and FeEDDHA were found to correlate with Fe reduction rates and reduction potentials (E0) of both chelates. Therefore, it is suggested that the reduction mechanism governs the Fe uptake process from PSB by dicots. Further studies will be conducted to determine the role of pH in Fe aquisition from PSB by monocots.

Key words

cotton iron uptake peanuts plant iron nutrition pseudobactin Pseudomonas putida redox potential siderophore sorghum sunflower 

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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • E. Bar-Ness
    • 1
  • Y. Chen
    • 1
  • Y. Hadar
    • 1
  • H. Marschner
    • 2
  • V. Römheld
    • 2
  1. 1.The Seagram Center for Soil and Water Sciences, Faculty of AgricultureThe Hebrew University of JerusalemRehovotIsrael
  2. 2.Institute for Plant NutritionHohenheim UniversityStuttgart 70Germany

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