Plant and Soil

, Volume 130, Issue 1–2, pp 231–241 | Cite as

Siderophores of Pseudomonas putida as an iron source for dicot and monocot plants

  • E. Bar-Ness
  • Y. Chen
  • Y. Hadar
  • H. Marschner
  • V. Römheld


Iron uptake from ferrated (59Fe) pseudobactin (PSB), a Pseudomonas putida siderophore, by various plant species was studied in nutrient solution culture under short term (10 h) and long term (3 weeks) conditions. In the short term experiments, 59Fe uptake rate from 59FePSB by dicots (peanuts, cotton and sunflower) was relatively low when compared with 59Fe uptake rate from 59FeEDDHA. Iron uptake rate from 59FePSB was pH and concentration dependent, as was the Fe uptake rate from 59FeEDDHA. The rate was about 10 times lower than that of Fe uptake from the synthetic chelate. Results were similar for long term experiments.

Monocots (sorghum) in short term experiments exhibited significantly higher uptake rate of Fe from FePSB than from FeEDDHA. In long term experiments, FePSB was less efficient than FeEDDHA as an Fe source for sorghum at pH 6, but the same levels of leaf chlorophyll concentration were obtained at pH 7.3.

Fe uptake rates by dicots from the siderophore and FeEDDHA were found to correlate with Fe reduction rates and reduction potentials (E0) of both chelates. Therefore, it is suggested that the reduction mechanism governs the Fe uptake process from PSB by dicots. Further studies will be conducted to determine the role of pH in Fe aquisition from PSB by monocots.

Key words

cotton iron uptake peanuts plant iron nutrition pseudobactin Pseudomonas putida redox potential siderophore sorghum sunflower 


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  1. Becker J O, Hedges R W and Messens E 1985a Inhibitory effect of pseudobactin on the uptake of Fe by higher plants. Appl. Environ. Microbiol. 49, 1090–1093.Google Scholar
  2. Becker J O, Messens E and Hedges R W 1985b Influence of agrobactin on the uptake of iron by plants. FEMS Microbiol. Ecol. 31, 171–175.Google Scholar
  3. Becker J O, Hedges R W and Messens E 1986 Diverse effects of some bacterial siderophores on the uptake of iron by plants. In Iron, Siderophores and Plant Disease. Ed. T R Swinburne. pp 61–70. NATO ASI series A: Life Sciences Vol. 117.Google Scholar
  4. Bienfait H F, Bino R J, van derBliek A M, Duivenvoorden J F and Fontaine J M 1983 Characterization of ferric reducing activity in roots of Fe-deficient Phaseolus vulgaris. Physiol. Plant. 59, 196–202.Google Scholar
  5. Bienfait H F, van derBriel M L and Mesland-Mul N T 1984 Measurement of the extracellular mobilizable iron pool in roots. J. Plant Nutr. 7, 659–665.Google Scholar
  6. Bienfait H F 1988 Mechanisms in Fe-efficiency reaction in higher plants. J. Plant Nutr. 11, 605–629.Google Scholar
  7. Bienfait H F 1989 Prevention of stress in iron metabolism of plants. Acta Bot. Neerl. 38, 105–129.Google Scholar
  8. Bossier P and Verstraete W 1988 Ecology of Arthrobacterial JG-9-detectable hydroxamate siderophores in soils. Soil Biol. Biochem. 18, 487–492.Google Scholar
  9. Chaney R L, Brown J C and Tiffin L O 1972 Obligatory reduction of ferric chelates in iron uptake by soybeans. Plant Physiol. 50, 208–213.Google Scholar
  10. Crowley D E, Reid C P P and Szaniszlo P J 1987 Microbial siderophores as iron source for plants. In Iron Transport in Microbes, Plants and Animals. Eds. GWinkelmann, D Van derHelm and J BNeilands. pp 375–386. VCH Verlagsgesellschaft, Weinheim, FRG.Google Scholar
  11. Crowley D E, Reid C P P and Szaniszlo J P 1988 Utilization of microbial siderophores in iron acquisition by oat. Plant Physiol. 87, 680–685.Google Scholar
  12. Jurkevitch E, Hadar Y and Chen Y 1988 Involvement of bacterial siderophores in the remedy of lime-induced chlorosis in peanuts. Soil Sci. Soc. Am. J. 52, 1032–1037.Google Scholar
  13. Kloepper J W, Leong J, Teintze M and Schroth M N 1980 Enhanced plant growth by siderophores produced by plant growth promoting rhizobacteria. Nature 286, 885–886.Google Scholar
  14. MacKinney G 1941 Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322.Google Scholar
  15. Marschner H, Römheld V and Kissel M 1986 Different strategies in higher plants in mobilization and uptake of iron. J. Plant Nutr. 9, 695–713.Google Scholar
  16. Meyer J M and Abdallah M A 1978 The fluorescent pigment of Pseudomonas fluorescens: Biosynthesis, purification and physicochemical properties. J. Gen. Microbiol. 107, 319–328.Google Scholar
  17. Orlando J A and Neilands J B 1982 Ferrichrome compounds as a sourse of iron for higher plants. In Chemistry and Biology of Hydroxamic Acids. Ed. HKehl. pp 123–129. S. Karger, Basel.Google Scholar
  18. Powell P E, Szaniszlo J P, Cline G R and Reid C P P 1982 Hydroxamate siderophores in the iron nutrition of plants. J. Plant Nutr. 5, 653–673.Google Scholar
  19. Price C A 1968 Iron compounds and plant nutrition. Annu. Rev. Plant Physiol. 19, 239–248.Google Scholar
  20. Raymond K N, Muller G and Matzanke B F 1984 Complexation of iron by siderophores: A review of their solution and structural chemistry and biological function. Topics Curr. Chem. 123, 49–102.Google Scholar
  21. Reid C P P, Crowley D E, Powell P E, Kim H J and P J Szaniszlo 1984 Utilization of iron by oat when supplied as ferrated hydroxamate siderophore or as a ferrated synthetic chelate. J. Plant Nutr. 7, 437–447.Google Scholar
  22. Reid R K, Reid C P P, Powell P E and Szanizlo P J 1984 Comparison of siderophore concentrations in aqueous extracts of rhizosphere and adjacent bulk soil. Pedologia 26, 263–266.Google Scholar
  23. Römheld V and HMarschner 1983 Mechanism of iron uptake by peanut plants. I. Fe(III) reduction, chelate spliting and release of phenolics. Plant Physiol. 71, 949–954.Google Scholar
  24. Römheld V 1987 Different strategies for iron acquisition by higher plants. Physiol. Plant 70, 231–234.Google Scholar
  25. Stutz E 1964 Aufnahme von Ferrioxamine B durch Tomatenpflanzen. Experimentia 20, 430–431.Google Scholar
  26. Takagi S 1976 Naturally occurring iron-chelating compounds in oat- and rice-roots washing. Soil Sci. Plant Nutr. 22, 423–433.Google Scholar
  27. Takagi S, Nomoto K and Takemoto T 1984 Physiological aspects of mugineic acid, a possible phytosiderophore of graminaceous plants. J. Plant Nutr. 7, 469–477.Google Scholar

Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • E. Bar-Ness
    • 1
  • Y. Chen
    • 1
  • Y. Hadar
    • 1
  • H. Marschner
    • 2
  • V. Römheld
    • 2
  1. 1.The Seagram Center for Soil and Water Sciences, Faculty of AgricultureThe Hebrew University of JerusalemRehovotIsrael
  2. 2.Institute for Plant NutritionHohenheim UniversityStuttgart 70Germany

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