Plant and Soil

, Volume 178, Issue 2, pp 247–253 | Cite as

The survival and development of inoculant ectomycorrhizal fungi on roots of outplanted Eucalyptus globulus Labill

  • B. D. Thomson
  • G. E. StJ. Hardy
  • N. Malajczuk
  • T. S. Grove
Research Article


The survival and development of two inoculant ectomycorrhizal fungi (Hebeloma westraliense Bough. Tom. and Mal. and Setchelliogaster sp. nov.) on roots of outplanted Eucalyptus globulus Labill. was examined at two expasture field sites in the south-west of Western Australia. Site 1 was a gravelly yellow duplex soil, and Site 2 was a yellow sandy earth. Plants were grown in steamed or unsteamed soil, in root bags designed as field containers for young growing trees. Three, 6 and 12 months after outplanting, plants were removed from these bags and assessed for dry weights of shoots and ectomycorrhizal colonization of roots.

The inoculant ectomycorrhizal fungi (identified on the basis of the colour and morphology of their mycorrhizas) survived on roots of E. globulus for at least 12 months after outplanting at both field sites. At Site 1, however, colonization of new fine roots by the inoculant fungi was low (less than 20% of fine root length). Inoculation had no effect on the growth of E. globulus at this site. In contrast, at Site 2 the inoculant ectomycorrhizal fungi colonized up to 30–50% of new fine root length during the first 6 months after outplanting. There was a corresponding growth response to ectomycorrhizal inoculation at this site, with a close relationship (r2=0.82**) between plant growth at 12 months and root colonization at 3 months. Plant growth at 12 months was related less closely with root colonization at 6 or 12 months. Root colonization by ‘resident’ ectomycorrhizal fungi increased with time at both field sites. At Site 2, this increase appeared to be at the expense of colonization by the inoculant fungi, which was reduced to less than 10% of fine root length at 12 months. Steaming the soil had little effect on colonization by the inoculant ectomycorrhizal fungi at either field site, but decreased colonization by the resident ectomycorrhizal fungi.

Key words

colonization ectomycorrhiza eucalypt inoculant survival 


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  1. Amaranthus M P and Perry D A 1987 Effect of soil transfer on ectomycorrhizal formation and the survival and growth of conifer seedlings on old, reforested clear-cuts. Can. J. For. Res. 17, 944–950.Google Scholar
  2. Bougher N L, Tommerup I C and Malajczuk N 1991 Nuclear behaviour in the basidiomes and ectomycorrhizas of Hebeloma westraliense sp. nov. Mycol. Res. 95, 683–688.Google Scholar
  3. Bowen G D and Theodorou C 1978 Interactions between bacteria and ectomycorrhizal fungi. Soil Biol. Biochem. 11, 119–126.Google Scholar
  4. Bray R H and Kurtz L T 1945 Determination of total, organic, and available forms of phosphorus in soils. Soil Sci. 59, 39–45.Google Scholar
  5. Castellano M A and Trappe J M 1991 Pisolithus tinctorius fails to improve plantation performance of inoculated conifers in south-western Oregon. New For. 5, 349–358.Google Scholar
  6. Chu-Chou M 1979 Mycorrhizal fungi of Pinus radiata in New Zealand. Soil Biol. Biochem. 11, 557–562.Google Scholar
  7. Chu-Chou M and Grace L J 1981 Mycorrhizal fungi of Pseudotsuga menziesii in the North Island of New Zealand. Soil Biol. Biochem. 13, 247–249.Google Scholar
  8. Dahlberg A 1990 Effect of soil humus cover on the establishment and development of mycorrhizae on containerized Pinus sylvestris L. and Pinus contorta spp. latifolia Engelm. after outplanting. Scand. J. For. Res. 5, 103–112.Google Scholar
  9. Dahlberg A and Stenstrom E 1991 Dynamic changes in nursery and indigenous mycorrhiza of Pinus sylvestris seedlings planted out in forest and clearcuts. Plant and Soil 136, 73–86.Google Scholar
  10. Danielson R M and Visser S 1989 Host response to inoculation and behaviour of introduced and indigenous ectomycorrhizal fungi of jack pine grown on oil-sands tailings. Can. J. For. Res. 19, 1412–1421.Google Scholar
  11. Dell B and Wallace I M 1983 Periodicity of fine root growth in jarrah (Eucalyptus marginata Donn ex Sm.). Aust. J. Bot. 31, 247–254.Google Scholar
  12. Dodd J C and Thomson B D 1994 The screening and selection of inoculant arbuscular-mycorrhizal and ectomycorrhizal fungi. Plant and Soil 159, 149–158.Google Scholar
  13. Garbaye J and Bowen G D 1987 Effect of different microflora on the success of ectomycorrhizal inoculation of Pinus radiata. Can. J. For. Res. 17, 941–943.Google Scholar
  14. Garbaye J, Delwaulle J C and Diangana D 1988 Growth response of eucalypts in the Congo to ectomycorrhizal inoculation. For. Ecol. Manage. 24, 151–157.Google Scholar
  15. Grove T S and Le Tacon F 1993 Mycorrhiza in plantation forestry. Adv. Plant Pathol. 9, 191–227.Google Scholar
  16. Grove T S and Malajczuk N 1994 The potential for management of ectomycorrhiza in forestry. In Management of Mycorrhizas in Agriculture, Horticulture and Forestry. Eds. A D Robson, L K Abbott and N Malajczuk. pp 201–210. Kluwer Academic Publishers, Dordrecht, the Netherlands.Google Scholar
  17. Grove T S, Malajczuk N, Burgess T, Thomson B D and Hardy G 1991 Growth responses of plantation eucalypts to inoculation with selected ectomycorrhizal fungi. Proc. IUFRO Symp. Int. For. 86–93.Google Scholar
  18. Harvey A E, Jurgensen M J, Larsen M J and Graham R T 1987 Relationships among soil microsite, ectomycorrhizae, and natural conifer regeneration of old-growth forests in western Montana. Can. J. For. Res. 17, 58–62.Google Scholar
  19. Last F T, Mason P A, Pelham J and Ingleby K 1984 Fruitbody production by sheathing mycorrhizal fungi: Effects of “host genotype” and propagating soils. For. Ecol. Manage. 9, 221–227.Google Scholar
  20. Malajczuk N, Molina R and Trappe J M 1982 Ectomycorrhiza formation in Eucalyptus I. Pure culture synthesis, host specificity and mycorrhizal compatibility with Pinus radiata. New Phytol. 91, 467–482.Google Scholar
  21. Mamoun M and Olivier J M 1993 Competition between Tuber melanosporum and other ectomycorrhizal fungi under two irrigation regimes I. Competition with Tuber brumale. Plant and Soil 149, 211–218.Google Scholar
  22. Martin F, Tommerup I C and Tagu D 1994 Genetics of ectomycorrhizal fungi: progress and prospects. Plant and Soil 159, 159–170.Google Scholar
  23. Marx D H, Bryan W C and Cordell C E 1977 Survival and growth of pine seedlings with Pisolithus ectomycorrhizae after two years on reforestation sites in north Carolina and Florida. For. Sci. 23, 363–373.Google Scholar
  24. Marx D H, Hedin A and Toe S F P 1985 Field performance of Pinus caribaea var hondurensis and fertilizer after three years on a savanna site in Liberia. For. Ecol. Manage. 13, 1–25.Google Scholar
  25. McAfee B J and Fortin J A 1987 The influence of pH on the competitive interactions of ectomycorrhizal mycobionts under field conditions. Can. J. For. Res. 17, 859–863.Google Scholar
  26. Newman E I 1966 A method for estimating the total length of a root in a sample. J. Appl. Ecol. 3, 139–145.Google Scholar
  27. Samson J and Fortin J A 1986 Ectomycorrhizal fungi of Larix laricina and the interspecific and intraspecific variation in response to temperature. Can. J. Bot. 64, 3020–3028.Google Scholar
  28. Stenstrom E and Ek M 1990 Field growth of Pinus sylvestris following nursery inoculation with mycorrhizal fungi. Can. J. For. Res. 20, 914–918.Google Scholar
  29. Thomson B D, Grove T S, Malajczuk N and Hardy G E StJ 1994 The effectiveness of ectomycorrhizal fungi in increasing the growth of Eucalyptus globulus Labill. in relation to root colonization and hyphal development in soil. New Phytol, 126, 517–524.Google Scholar
  30. Villeneuve N, Le Tacon F and Bouchard D 1991 Survival of inoculated Laccaria bicolor in competition with native ectomycorrhizal fungi and effects on the growth of outplanted Douglas-fir seedlings. Plant and Soil 135, 95–107.Google Scholar
  31. Walkley A 1947 A rapid method for determining organic carbon in soils. Soil Sci. 63, 251–264.Google Scholar

Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • B. D. Thomson
    • 1
  • G. E. StJ. Hardy
    • 2
  • N. Malajczuk
    • 1
  • T. S. Grove
    • 1
  1. 1.CSIRO Division of ForestryWembleyAustralia
  2. 2.Bunnings TreefarmsManjimupAustralia

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