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Plant and Soil

, Volume 173, Issue 2, pp 233–238 | Cite as

Growth enhancement of Citrus reshni after inoculation with Glomus intraradices and Trichoderma aureoviride and associated effects on microbial populations and enzyme activity in potting mixes

  • A. Camprubí
  • C. Calvet
  • V. Estaún
Research Article

Abstract

There have been some scientific reports suggesting that dual inoculations with arbuscular mycorrhizal (AM) and saprophytic soil fungi may cause an additive or synergistic growth enhancement of the inoculated host plant. Some Trichoderma spp. have shown antagonistic potential against pathogenic fungi and a beneficial effect on plant growth. Joint inoculations of the mycorrhizal fungus Glomus intraradices Schenck and Smith, isolated from a citrus nursery (Tarragona, Spain) and a strain of Trichoderma aureoviride Rifai, isolated from an organic compost, were tested on a citrus rootstock, Citrus reshni Hort. ex Tan. The interactions between both microorganisms and their influence on mycorrhizal root colonization and plant growth enhancement, the changes produced in the soil microbial activity, like esterase, trehalase, phosphatase and chitinase activities, and on microbial populations were evaluated in three organic substrates: (1) sphagnum peat and autoclaved sandy soil (1/1, v/v), (2) sphagnum peat, quartz sand and perlite (1/1/1, v/v) and (3) pine bark compost (BVU, Prodeasa Product). Substrate characteristics were more important than the AM inoculation treatment in the determination of enzyme activity. In bark compost, the number of bacterial colonies obtained on soil-dilution plates was significantly higher than in peat and sand mixtures. Inoculation with T. aureoviride alone produced no significant effect on growth enhancement of C. reshni. However, dual inoculation with both, T. aureoviride and G. intraradices significantly increased plant growth in two of the substrates used and was the best treatment in pine bark amended compost. The inoculation with T. aureoviride did not affect the development of mycorrhizal root colonization. These results show a synergistic effect of G. intraradices and T. aureoviride on the growth of C. reshni in organic substrates and indicate the potential benefits of using combined inoculations.

Key words

citrus rootstocks Glomus intraradices mycorrhiza soil enzyme activity Trichoderma aureoviride 

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References

  1. Ames R N 1989 Mycorrhiza development in onion in response to inoculation with chitin-decomposing actinomycetes. New Phytol. 112, 423–427.Google Scholar
  2. Azcón-Aguilar C, Barea J M and Olivares J 1980 Effects of Rhizobium polysaccharides on VA mycorrhiza formation. In Second International Symposium on microbial Ecology. Abstr. No.187. University of Warwick, Coventry, UK.Google Scholar
  3. Azcón-Aguilar C and Barea J M 1985 Effect of soil microorganisms on the formation of vesicular-arbuscular mycorrhiza. Trans. Br. Mycol. Soc. 84, 536–537.Google Scholar
  4. Azcón-Aguilar C, Díaz-Rodríguez R M and Barea J M 1986 Effect of soil microorganisms on spore germination and growth of the vesicular-arbuscular mycorrhizal fungus Glomus mosseae. Trans. Br. Mycol. Soc. 86, 337–340.Google Scholar
  5. Azcón-Aguilar C and Barea J M 1992 Interactions between mycorrhizal fungi and other rhizosphere microorganisms. In Mycorrhizal functioning. An Integrative Plant-Fungal Process. Ed. M J Allen. Chapman and Hall, New York, London.Google Scholar
  6. Baker R 1989 Improved Trichoderma spp. for promoting crop productivity. Tibtech. 7, 34–38.Google Scholar
  7. Barber D A and Martin J K 1976 The release of organic substances by cereal roots into soil. New Phytol. 76, 69–80.Google Scholar
  8. Beam H W 1971 Effect of fluometuron and prometryne on β-galactosidase and phosphatase produced by Rhizoctonia solani Kuhn in soil culture. M.S. thesis, Auburn University, USA.Google Scholar
  9. Bowen G D 1980 Misconceptions, concepts and approaches in rhizosphere biology. In Contemporany Microbial Ecology. Eds. D C Ellwood, M J Latham, J N Hedger, J N Lynch and J M Slater. pp 283–304. Academic Press, London.Google Scholar
  10. Brundrett M C, Piché Y and Peterson R L 1984 A new method for observing the morphology of vesicular-arbuscular mycorrhizae. Can. J. Bot. 62, 2128–2134.Google Scholar
  11. Calvet C, Barea J M and Pera J 1992 In vitro interactions between the vesicular-arbuscular fungus Glomus mosseae and some saprophytic fungi isolated from organic substrates. Soil Biol. Biochem. 24, 775–780.Google Scholar
  12. Calvet C, Pera J and Barea J M 1993 Growth response of marigold (Tagetes erecta L.) to inoculation with Glomus mosseae, Trichoderma aureoviride and Pythium ultimum in a peat-perlite mixture. Plant and Soil 148, 1–6.Google Scholar
  13. Camprubí A 1994 Mycorrhizae in citrus nurseries: fungi characterization and selection. Introduction of the inoculation technology in agronomic plant production systems. Ph.D. thesis, University of Barcelona, Spain.Google Scholar
  14. Davey C B 1971 Nonpathogenic organisms associated with mycorrhizae. In Mycorrhizae. Ed. X Hacskaylo. p 255. USDA Miscellaneous Publication 1189.Google Scholar
  15. Dhruva Kumar J H A, Sharna G D and Mishra R R 1992 Soil microbial population numbers and enzyme activities in relation to altitude and forest degradation. Soil Biol. Biochem. 24, 761–767.Google Scholar
  16. Gopalakrishna N N 1980 Interaction between VA mycorrhiza and phosphate solubilizing fungi and their effects on rhizosphere microflora and growth of finger miller. M. Sc. (Agric.) thesis, University of Agricultural Sciences, Bangalore, India.Google Scholar
  17. Inbar Y, Boehm M J and Hoitink H A J 1991 Hydrolysis of fluorescein diacetate in sphagnum peat container media for predicting suppressiveness to damping-off caused by Pythium ultimum. Soil Biol. Biochem. 19, 95–99.Google Scholar
  18. Johnson L F and Curl E A 1972 Methods for research on ecology of soil-borne pathogens. Burgess Publishing Company, Minneapolis, Minesota.Google Scholar
  19. Koske R E and Gemma J N 1989 A modified procedure for staining roots to detect VA mycorrhizas. Mycol. Res. 92, 486–505.Google Scholar
  20. Kwok O C H, Fahy P C, Hoitink H A J and Kuter G A 1987 Interactions between bacteria and Trichoderma hamatum in suppression of Rhizoctonia damping-off in bark compost media. Phytopathology 77, 1206–1212.Google Scholar
  21. Manjunath A, Mohan R and Bagyaraj D J 1981 Interactions between Beijerinckia mobilis, Aspergillus niger and Glomus fasciculatum and their effects on growth of onion. New Phytol. 87, 723–727.Google Scholar
  22. Mellor R B 1992 Is trehalose a symbiotic determinant in symbioses between higher plants and microorganisms? Symbiosis 12, 113–129.Google Scholar
  23. Meyer J R and Linderman R G 1986 Response of subterraneum clover to dual inoculation with vesicular-arbuscular mycorrhizal fungi and a plant-growth promoting bacterium Pseudomonas putida. Soil Biol. Biochem. 18, 185–190.Google Scholar
  24. Nelson N 1944 A photometric adaptation of the Somogyi method for determination of glucose. J. Biol. Chem. 153, 375–380.Google Scholar
  25. Nemec S 1987 VA mycorrhizae in horticultural systems. In Ecophysiology of Va Mycorrhizal Plants. Eds. Gene and Safir. pp 193–211. CRC Press, Boca Raton, Florida.Google Scholar
  26. Pera J and Calvet C 1989 Suppression of Fusarium wilt of carnation in a composted pine bark and a composted olive pumice. Plant Dis. 73, 699–700.Google Scholar
  27. Schnurer J and Roswall T 1982 Fluorescein diacetate hydrolisis as a measure of total microbial activity in soil and litter. Appl. Environ. Microbiol. 43, 256–1261.Google Scholar
  28. Smith R E and Rodríguez-Kábana R 1982 The extraction and assay of soil trehalase. Plant and Soil 65, 335–344.Google Scholar
  29. Spanu P, Boller T, Ludwig A, Wiemken A, Faccio A and Bonfante-Fasolo P 1989 Chitinase in roots of mycorrhizal Allium porrum: regulation and localization. Planta 177, 447–455.Google Scholar
  30. Trillas-Gay M I, Hoitink H A J and Madden L V 1986 Nature of suppression of Fusarium wilt of radish in container medium amended with composted hardwood bark. Plant Dis. 70, 1023–1027.Google Scholar
  31. Wilson G M T, Hetrick B A D and Kitt D G 1989 Suppression of vesicular-arbuscular mycorrhizal fungus spore germination by nonsterile soil. Can. J. Bot. 67, 18–23.Google Scholar
  32. Windham M T, Elad Y and Baker R 1986 A mechanism for increased plant growth induced by Trichoderma spp. Phytopathology 76, 518–521.Google Scholar

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • A. Camprubí
    • 1
  • C. Calvet
    • 1
  • V. Estaún
    • 1
  1. 1.Institut de Recerca i Technologia Agroalimentaries (IRTA), Departament de Patologia VegetalCentre de CabrilsBarcelonaSpain

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