Incorporation of P32 in phosphate esters of the sugar cane plant and the effect of Si and Al on the distribution of these esters
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Summary
Good separation of phosphate esters labeled with P32 was obtained on paper-chromatography using the ascending technique in two dimensions at right angles.
The pattern of phosphate esters in chromatographic resolution was similar for both the roots and leaves of sugar cane but there were differences in the relative proportions of the individual esters. Significant amounts of some esters, of which G-6-P constituted the main bulk, were labeled and identified after short incubation periods of excised roots in the radioactive phosphate solution.
Although Al pre-treatment at the level of 0.5 mM Al2(SO4)3 stimulated labeled phosphate uptake, there was no effect on the extent of phosphorylation. With Si pre-treatment, however, a significant increase in the degree of phosphorylation was noted although no effect was obtained on labeled phosphate uptake
Keywords
Phosphate Sugar Ester Plant Physiology Cane
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Literature cited
- 1.Alexander, A. G., Sucrose-enzyme relationships in immature sugar cane as affected by variable nitrate and potassium supplied in sand culture. J. Agr. Univ. P. R. 48 (3), 165–231 (1964).Google Scholar
- 2.Alexander, A. G., Comparative studies of yeasts and sugar cane invertase. J. Agr. Univ. P.R. 53 (1), 41–56 (1969).Google Scholar
- 3.Ayres, A. S., Calcium silicate slag as a growth stimulant on low silicon soils. Soil Sci. 101, 216–227 (1966).Google Scholar
- 4.Bandurski, R. S. and Axelrod, B., chromatography of biologically important esters. J. Biol. Chem. 193, 404–410 (1952).Google Scholar
- 5.Bieleski, R. L. and Young, R. E., Extraction and separation of phosphate esters from plant tissues. Anal. Biochem. 6, 54–68 (1963).Google Scholar
- 6.Clarkson, D. T., Effect of aluminium on the uptake and metabolism of phosphorus by barley seedlings. Plant Physiol. 41, 165–172 (1966).Google Scholar
- 7.Clements, H. F., The rôles of calcium silicate slags in sugar cane growth. Rep. Hawaiian Sug. Technol. 24, 103–126 (1965).Google Scholar
- 8.Eaton, S. V., Effects of phosphorus deficiency on growth and metabolism of soya bean. Botan. Gaz. 111, 426 (1950).Google Scholar
- 9.Fisher, R. A., A preliminary note on the effect of sodium silicate in increasing the yield of barley. J. Agr. Sci. 19, 132–139 (1929).Google Scholar
- 10.Ginsburg, V., Stumpf, F. K., and Hassad, W. Z., The isolation of uridine diphosphate derivatives of D-glucose, D-galactose, D-xylose and L-arabinose from mungbean seedlings. J. Biol. Chem. 233, 977 (1956).Google Scholar
- 11.Ishibashi, H. and Akiyama, K., Physiological functions of silica in rice plants. Rep. 111. Bull. Fac. Agr. Yamaguchi Univ. 11 (cited by Comhaire, M., 1966. Agr. Dig. No. 7, 9–19) (1960).Google Scholar
- 12.Jackson, P. C. and Hagen, C. E., Products of orthophosphate absorption by barley root. Plant Physiol. Lancaster 35, 326–332 (1960).Google Scholar
- 13.Loughman, B. C. and Russell, R. S., The absorption and utilization of phosphate by young barley plants. J. Expt. Botany 8, 280–293 (1957).Google Scholar
- 14.Miettinen, J. K. and Savioja, T., Uptake of orthophosphate by normal and P-deficient pea plants. Suomen Kemistilehti 31, 84–90 (1958).Google Scholar
- 15.Okuda, A. and Takahashi, E., Le rôle du silicium. Agron. Trop. Nogent. 19, 534 (1964).Google Scholar
- 16.Raupach, M. and Piper, C. S., The interaction of silicate and phosphate in a lateritic soil. Australian J. Agr. Research 20, 818–831 (1959).Google Scholar
- 17.Tiffin, L. O. and Brown, J. C., Selective absorption from iron chelates by soya bean plants. Plant Physiol. 36, 710–714 (1961).Google Scholar
- 18.Wong You Cheong, Y. and Halais, P., Needs of sugar cane for silicon when growing in highly weathered soils. Exptl. Agr. 6, 99–106 (1970).Google Scholar
- 19.Wong You Cheong, Y., Heitz, A., and Deville, J., The effect of silicon on enzyme activity in vitro and sucrose production in sugar cane leaves. (In press.) Proc. I.S.S.C.T., Louisiana (1971).Google Scholar
- 20.Wright, K. E., The initial precipitation of phosphate in relation to aluminium toxicity. Plant Physiol. 18, 708–711 (1943).Google Scholar
- 21.Wright, K. E. and Donahue, B. A., Aluminium toxicity studies with radioactive phosphorus. Plant Physiol. 28, 674–679 (1953).Google Scholar
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