Studies of two Frankia strains isolated from Trevoa trinervis Miers
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The Frankia strains TtI 11 and TtI 12 isolated from T. trinervis Miers were characterized regarding their carbon source utilization, intrinsic antibiotic resistance, infectivity, and effectivity on the original host. Both strains grew on BAP medium supplemented with glucose, maltose, and sucrose, but differed in their ability to use other carbon sources such as propionate, pyruvate, acetate, succinate, citrate, and mannitol.
The isolates were sensitive to five of the twelve antibiotics tested at 1 μg mL−1 concentration: chloramphenicol, tobramycin, eritromycin, streptomycin, and rifampicin. They exhibited a variable degree of resistance at 1 μg mL−1 concentraction to penicillin G, 4-fluorouracil, oleandomycin, and lincomycin.
Both isolates were able to infect and nodulate the original host plant, and thus represent the first reported infective and effective microsymbionts for T. trinervis Miers, a rhamnaceous actinorhizal host. R O D Dixon Section editor
Key wordsantibiotic resistance carbon source Frankia Trevoa trinervis
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