Plant and Soil

, Volume 179, Issue 2, pp 261–268 | Cite as

Flooding-induced membrane damage, lipid oxidation and activated oxygen generation in corn leaves

  • Bin Yan
  • Qiujie Dai
  • Xiaozhong Liu
  • Shaobai Huang
  • Zixia Wang
Article

Abstract

Flooding effects on membrane permeability, lipid peroxidation and activated oxygen metabolism in corn (Zea mays L.) leaves were investigated to determine if activated oxygens are involved in corn flooding-injury. Potted corn plants were flooded at the 4-leaf stage in a controlled environment. A 7-day flooding treatment resulted in a significant increase in chlorophyll breakdown, lipid peroxidation (malondialdehye content), membrane permeability, and the production of superoxide (O 2 - ) and hydrogen peroxide (H2O2) in corn leaves. The effects were much greater in older leaves than in younger ones. Spraying leaves with 8-hydroxyquinoline (an O 2 - scavenger) and sodium benzoate (an .OH scavenger) reduced the oxidative damage and enhanced superoxide dismutase (SOD) activity. A short duration flooding treatment elevated the activities of SOD, catalase, ascorbate peroxidase (AP), and glutathione reductase (GR), while further flooding significantly reduced the enzyme activities but enhanced the concentrations of ascorbic acid and reduced form glutathione (GSH). It was noted that the decline in SOD activity was greater than that in H2O2 scavengers (AP and GR). The results suggested that O 2 - induced lipid peroxidation and membrane damage, and that excessive accumulation of O 2 - is due to the reduced activity of SOD under flooding stress.

Key words

activated oxygen activated oxygen scavenging system flooding lipid peroxidation membrane injury Zea mays 

Abbreviations

AP

ascorbate peroxidase

ASA

ascorbic acid

CAT

catalase

DDTT

sodium diethyldithiocarbamate

H2O2

hydrogen peroxide

8-HQ-8

hydroxyquinoline

GR

glutathione reductase

GSH

reduced form glutathione

MDA

malondialdehye

O2-

superoxide

.OH

hydroxyl radical

SB

sodium benzoate

SOD

superoxide dismutase

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Copyright information

© Kluwer Academic Publishers 1996

Authors and Affiliations

  • Bin Yan
    • 1
  • Qiujie Dai
    • 1
  • Xiaozhong Liu
    • 1
  • Shaobai Huang
    • 1
  • Zixia Wang
    • 1
  1. 1.Institute of Agrobiological Genetics and PhysiologyJiangsu Academy of Agricultural SciencesNanjing, JiangsuP. R. China

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