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Plant and Soil

, Volume 163, Issue 2, pp 243–255 | Cite as

Distribution of soil fractions and location of soil bacteria in a vertisol under cultivation and perennial grass

  • M. Kabir
  • J. L. Chotte
  • M. Rahman
  • R. Bally
  • L. Jocteur Monrozier
Research Article

Abstract

Effects of soil management on soil characteristics were investigated on the rhizosphere (RPP) and the nonrhizosphere (NRPP) soil of a re-grass vertisol underDigitaria decumbens and in the soil under continuous cultivation (CC). A low energy technique allowed to separate eight size and density fractions, including macro- and micro-aggregates while preserving soil bacteria. Organic C and N, microbial biomass C and the number of total bacteria (AODC) and ofAzospirillum brasilense and their distribution were determined in soil fractions isolated from the CC, NRPP and RPP soils. Soil macroaggregates (>2000 μm) were similarly predominant in the NRPP and RPP soils when the dispersible clay size fraction (<2 μm) respresented more than 25% of the CC soil mass. The main increase of C content in RPP originated from the macroaggregates (> 2000 μm) and from the root fraction, not from the finer separates. The proportion of organic C as microbial biomass C revealed the low turnover of microbial C in the PP situations, especially in the clay size fraction of the NRPP soil. A common shift of AODC toward the finer separates from planted soils (CC and RPP) revealed the influence of living plants on the distribution of soil bacteria. The relative abundance ofA. brasilense showed the presence of the active roots ofDigitaria in the macroaggregates and their contact with the dispersible clay size fraction of the rhizosphere soil.

Key words

Azospirillum brasilense Digitaria decumbens microbial biomass C regrass soil rhizosphere soil fractionation vertisol 

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References

  1. Amato M and Ladd J N 1988 Assay for biomass based on ninhydrine reactive nitrogen in extracts of fumigated soils. Soil Biol. Biochem. 20, 107–114.Google Scholar
  2. Amato M and Ladd J N 1992 Decomposition of14C-labelled glucose and legume material in soils: properties influencing the accumulation of organic residue C and microbial biomass C. Soil Biol. Biochem. 24, 455–464.Google Scholar
  3. Angers D A, N'dayegamiye A and Côté D 1993 Tillage-induced differences in organic matter of particle-size fractions and microbial biomass. Soil Sci. Soc. Am. J. 57, 512–516.Google Scholar
  4. Balandreau J and Knowles R 1978 The rhizosphere.In Interactions between non-pathogenic soil microorganisms and plants. Eds. Y R Dommergues and S V Krupa. pp 243–266. Elsevier Scientific Publishing Company, New-York.Google Scholar
  5. Cambardella C A and Elliott E T 1992 Particulate soil organic-matter changes across a grassland cultivation sequence. Soil Sci. Soc. Am. J. 56, 777–783.Google Scholar
  6. Catroux G and Schnitzer M 1987 Chemical, spectroscopic, and biological characteristics of the organic matter in particle size fractions separated from an Aquoll. Soil Sci. Soc. Am. J. 51, 1200–1207.Google Scholar
  7. Chaussod R, Nicolardot B, Catroux G and Chretien J 1986 Relationships between physico-chemical and biological characteristics of a few cultivated soils. Science du Sol 24, 213–221.Google Scholar
  8. Chotte J L, Jocteur Monrozier L, Villemin G and Albrecht A 1992 Soil microhabitats and the importance of the fractionation method.In Soil Organic Matter Dynamics and Sustainability of Tropical Agriculture. Eds. K Mulongoy and R Merckx. pp 39–45. Wiley-Sayce Publishers, IITA/KU Leuven, The Netherlands.Google Scholar
  9. Chotte J L, Villemin G, Guilloré P and Jocteur Monrozier L 1993 Morphological aspects of microorganism habitats in a vertisol.In International Workshop on Soil Micromorphology (Townsville, Australia, 12–17 July 1992). Elsevier, Amsterdam, The Netherlands.Google Scholar
  10. Christensen B T 1992 Physical fractionation of soil and organic matter in particle size and density separates. Adv. Soil Sci. 20, 1–90.Google Scholar
  11. Collins H P, Rasmussen P E and DouglasJr C L 1992 Crop rotation and residue management effects on soil carbon and microbial dynamics. Soil Sci. Soc. Am. J. 56, 783–788.Google Scholar
  12. Dorioz J M and Robert M 1987 Aspects microscopiques des relations entre les microorganismes ou les végétaux et les argiles. Conséquences sur les microorganisations et sur la microstruturation des sols.In Soil Micromorphology. Eds. N Fedoroff, L Bresson and M A Courty. pp 353–361. AFES, Paris, France.Google Scholar
  13. Day J M and Döbereiner J 1976 Physiological aspects of N2 fixation by aSpirillum ofDigitaria roots. Soil Biol. Biochem. 8, 45–50.Google Scholar
  14. Döbereiner J and Pedrosa F O 1987 Nitrogen fixing bacteria in nonleguminous crop plants. Soil Sci. Soc. Am. Proc. 43, 951–955.Google Scholar
  15. Duchaufour P 1977 Dynamique de la matière organique.In Pédologie 1: Pédogénèse et classification. Eds. P Duchaufour et B Souchier. pp 28–70. Masson, Paris, France.Google Scholar
  16. Elliott E T 1986 Aggregate structure and carbon, nitrogen, and phosphorus in native and cultivated soils. Soil Sci. Soc. Am. Proc. 50, 627–633.Google Scholar
  17. Foster R C 1988 Microenvironments of soil microorganisms. Biol. Fertil. Soils 6, 189–203.Google Scholar
  18. Foster R C, Rovira A D and Cock T W 1983 Ultrastructure of the root-soil interface. The American Phytopathological Society, St Paul, Minnesota, USA. 156p.Google Scholar
  19. Gamard P 1991 Etude de populations d'Azospirillum dans la rhizosphère; Caractérisation sérologique et activité dénitrifiante. Thèse Univ. Lyon 1, France. 173p.Google Scholar
  20. Gray T R G and Williams S T 1971 Microbial productivity in soils. Gen. Microbiology Soc. Symposium. pp 255–286.Google Scholar
  21. Hart P B S, August J A, Ross C W and Jullian J F 1988 Some biochemical and physical properties of Tokomaro silt loam under pasture and after 10 years of cereal cropping. New-Zeal. J. Agric. Res. 31, 77–86.Google Scholar
  22. Hassink J, Bouwman L A, Zwart K B and Brussaard L 1993 Relationships between habitable pore space, soil biota and mineralization in grassland soils. Soil Biol. Biochem. 25, 47–55.Google Scholar
  23. Hattori T 1988 Soil aggregates as microhabitats of microorganisms. Rep. Inst. Agr. Res. Tohoku Univ. 37, 23–36.Google Scholar
  24. Haynes R J and Swift R S 1990 Stability of soil aggregates in relation to organic constituents and soil water content. J. Soil Sci. 41, 73–83.Google Scholar
  25. Jocteur Monrozier L, Ladd J N, Fitzpatrick R W, Foster R C and Raupach M 1991 Components and microbial biomass content of size fractions in soils of contrasting aggregation. Geoderma 49, 37–62.Google Scholar
  26. Ladd J N and Amato M 1988 Relationships between biomass14C of a range of fumigate soils. Soil Biol. Biochem 20, 115–116.Google Scholar
  27. Ladd J N and Foster R M 1987 Role of soil microflora in nitrogen turn-over.In Advances in Nitrogen Cycling in Agricultural Ecosystems. Ed. J R Wilson. pp 113–133. CAB International, Wallingford, Oxon, UK.Google Scholar
  28. Mahboubi A A, Lal R and Faussey N R 1993 Twenty-eight years of tillage effects on two soils in Ohio. Soil Sci. Soc. Am. J. 57, 506–512.Google Scholar
  29. Oades J M 1988 The retention of organic matter in soils. Biogeochemistry 5, 35–70.Google Scholar
  30. O'Hara G W, Davey M R and Lucas J A 1983 Association between the nitrogen fixing bacteriumA. brasilense and excised plant roots. Z. Pflanze. Physiol. Bd. 113, 1–13.Google Scholar
  31. Patriquin D G, Döbereiner J and Jain D K 1983 Sites and processes of association between diazotrophs and grasses. Can. J. Microbiol. 29, 900–915.Google Scholar
  32. Postma J, Hok-a-hin C H and VanVeen J A 1990 Role of microniches in protecting introducedRhizobium leguminosarum biovartrifolii against competition and predation in soil. Appl. Environ. Microbiol. 56, 495–502.Google Scholar
  33. Ramsay A J and Bawden A D 1983 Effects of sterilisation and storage on respiration, nitrogen status and direct counts of soil bacteria using acridine orange. Soil Biol. Biochem. 15, 263–268.Google Scholar
  34. Reinhold B, Hurck T, Niemann E G and Fendrik I 1986 Close association ofAzospirillum and diazotrophic rods with different root zones of kallar grass. Appl. Environ. Microbiol. 52, 520–526.Google Scholar
  35. Rutherford P M and Juma N G 1992 Influence of texture on habitable pore space and bacterial-protozoan populations in soil. Biol. Fertil. Soils 12, 221–227.Google Scholar
  36. Russel E W 1973 Soil Conditions and Plant Growth. Longman, London, 849 p.Google Scholar
  37. Santruckova H and Straskraba M 1991 On the relationship between specific respiration activity and microbial biomass in soils. Soil Biol. Biochem. 23, 525–532.Google Scholar
  38. Schank C, Bouton J H, Weiser G C, Zuberer D A, Tyler M E, Milam J R and Littell R C 1979 Fluorescent antibody technique to identifyA. brasilense associated with roots of grasses. Soil Biol. Biochem. 11, 287–295.Google Scholar
  39. Scherba G, Weige R M and O'BrienJr W D 1991 Quantitative assessment of the germicidal efficacy of ultrasonic energy. Appl. Environ. Microbiol. 57, 2079–2084.Google Scholar
  40. Schmidt E 1974 Quantitative autoecological study of microorganisms in soil by immunofluorescence. Soil Science 118, 141–149.Google Scholar
  41. Smith M S and Tiedje J M 1979 The effect of roots on soil denitrification. Soil Sci. Soc. Am. Proc. 43, 951–955.Google Scholar
  42. Sparling G P, Ord B G and Vaughan D 1981 Changes in microbial biomass and activity in soils amended with phenolic acids. Soil Biol. Biochem. 13, 455–460.Google Scholar
  43. Sparling G P and Cheshire M V 1985 Effect of periodate oxidation on the polysaccharide content and microaggregate stability of rhizosphere and non-rhizosphere soils. Plant and Soil 88, 113–122.Google Scholar
  44. Stotzky G and Burns R G 1982 The soil environment: clay-humus microbe interaction.In Experimental Microbial Ecology. Eds. R G Burns and J H Slater. pp 105–133. Blackwell Scientific, Oxford, UK.Google Scholar
  45. Tiessen H and Stewart J W B 1983 Particle size fractions and their studies of soil organic matter. II. Cultivation effects on organic matter composition in size fractions. Soil Sci. Soc. Am. J. 47, 509–514.Google Scholar
  46. Tiessen H, Karamanos R E, Stewart J W B and Selles F 1984 Natural15N abundance as an indicator of soil organic transformations in native and cultivated soils. Soil Sci. Soc. Am. J. 48, 312–315.Google Scholar
  47. Tisdall J M and Oades J M 1982 Organic matter and water stable aggregates in soil. J. Soil Sci. 32, 141–163.Google Scholar
  48. Turchenek L W and Oades J M 1979 Fractionation of organo-mineral complexes by sedimentation and density techniques. Geoderma 21, 311–343.Google Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • M. Kabir
    • 1
  • J. L. Chotte
    • 2
  • M. Rahman
    • 3
  • R. Bally
    • 1
  • L. Jocteur Monrozier
    • 1
  1. 1.Laboratoire d'Ecologie microbienne des sols, CNRS URA 1450Université Lyon 1Villeurbanne CedexFrance
  2. 2.Laboratoire Matière Organique des Sols TropicauxORSTOMFort de France cedex
  3. 3.Laboratory of Soil Microbiology, Department of Soil ScienceUniversity of DahkaDakhaBangladesh

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