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Hydrobiologia

, Volume 277, Issue 3, pp 145–158 | Cite as

Stimulation of aquatic bacterial activity by cyanobacteria

  • Lizhu Wang
  • John C. Priscu
Article

Abstract

The time-course response of natural bacterial populations and isolates from lake water to various densities of the filamentous cyanobacteriaAphanizomenon flos-aquae andLyngbya birgei collected from the same lake is reported. The cyanobacteria were separated from the bacteria by dialysis membranes that allowed only dissolved cyanobacterial products to pass. Bacterial3H-thymidine incorporation and cell number were significantly (p<0.05) correlated with cyanobacterial density for both species. Estimated dissolved organic carbon (DOC) utilization, based on bacterial biomass changes over time, were usually significantly (p<0.01) correlated with cyanobacterial density and the decrease in bulk pool DOC for both species. Bacterial volume per cell increased significantly (p<0.05) in response to cyanobacterial density on day 5 of the experiments; cell volume remained unchanged on day 1. Bacterial cell numbers on outer surfaces of the tubular membrane containing the cyanobacteria (on the side exposed to the test bacteria) were significantly (p<0.01) correlated with cyanobacterial density. Statistical analysis inferred that bacteria closely associated with cyanobacteria (i.e. attached) responded more strongly to cyanobacterial products than free-living bacteria. Overall, our results indicate that cyanobacterial products have a potentially important role in regulating bacterioplankton productivity in aquatic systems.

Key words

bacterioplankton cyanobacteria extracellular products bacterial stimulation 

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References

  1. Bell, W. H. & E. Sakshaug, 1980. Bacterial utilization of algal extracellular products. 2. A kinetic study of natural populations. Limnol. Oceanogr. 25: 1021–1033.Google Scholar
  2. Bird, D. F. & J. Kalff, 1984. Empirical relationships between bacterial abundance and chlorophyll concentration in fresh and marine waters. Can. J. Fish. Aquat. Sci. 41: 1015–1023.Google Scholar
  3. Bratak, G., 1985. Bacterial biovolume and biomass estimations. Appl. Environ. Microbiol. 49: 1488–1493.Google Scholar
  4. Chrost, R. J., 1978. The estimation of extracellular release by phytoplankton and hetero trophic activity of aquatic bacteria. Acta. Microbiol. Pol. 27: 139–146.PubMedGoogle Scholar
  5. Chrzanowski, T. H. & J. G. Hubbard, 1989. Bacterial utilization of algal extracellular products in a southwestern reservoir. Hydrobiologia 179: 61–71.Google Scholar
  6. Cole, J. J., G. E. Likens & D. L. Strayer, 1982. Photosynthetically produced dissolved organic carbon: An important carbon source for planktonic bacteria. Limnol. Oceanogr. 27: 1080–1090.Google Scholar
  7. Coveney, M. F., 1982. Bacterial uptake of photosynthetic carbon from freshwater phyto plankton. Oikos 38: 8–20.Google Scholar
  8. Derenbach, J. B. & P. J. Leb. Williams, 1974. Autotrophic and bacterial production: frac tionation of plankton populations by different filtration of samples from the English Channel. Mar. Biol. 25: 263–269.CrossRefGoogle Scholar
  9. Dixon, W. J. & F. J. Massey, 1983. Introduction to statistical analysis. McGraw-Hill Book Company, New York: 227–231.Google Scholar
  10. Feuillade, M., Ph. Dufour & Feuillade, 1988. Organic carbon release by phytoplankton and bacterial reassimilation. Schweiz. Z. Hydrol. 50: 115–135.Google Scholar
  11. Fuhrman, J. A., J. W. Ammerman & F. Azam, 1980. Bacterioplankton in the coastal eutrophic zone: distribution, activity and possible relationship with phytoplankton. Mar. Biol. 60: 201–207.CrossRefGoogle Scholar
  12. Herbst, V. & Y. Overbeck, 1978. Metabolic coupling between algaOscillatoria redekei and accompanying bacteria. Naturwissenschaften 65: 598–608.CrossRefGoogle Scholar
  13. Hobbie, J. E., J. Dalay & S. Jasper, 1977. Use of nucleopore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33: 1225–1228.PubMedGoogle Scholar
  14. Imam, S. H., R. F. Bard & T. R. Tosteson, 1984. Specificity of marine microbial surface interactions. Appl. Envir. Microbiol. 47: 833–839.Google Scholar
  15. Jensen, L. M., 1983. Phytoplankton release of extracellular organic carbon, molecular weight composition, and bacterial assimilation. Mar. Ecol. Prog. Ser. 11: 39–48.Google Scholar
  16. Jensen, L. M. & M. Sondergaard, 1985. Comparison of two methods to measure algal release of dissolved organic carbon and the subsequent uptake by bacteria. J. Plank ton Res. 7: 41–56.Google Scholar
  17. Lampert, W., 1978. Release of dissolved organic carbon by grazing zooplankton. Limnol. Oceanogr. 23: 831–834.CrossRefGoogle Scholar
  18. Larsson, U. & A. Hagström, 1979. Phytoplankton exudate release as an energy source forthe growth of pelagic-bacteria. Mar. Biol. 52: 199–296.CrossRefGoogle Scholar
  19. Larsson, U. & A. Hagström, 1982. Fractionated phytoplankton primary production, exu date release, and bacterial production in a Baltic eutrophication gradient. Mar. Biol. 67: 57–70.CrossRefGoogle Scholar
  20. Marshall, K. C. 1992. Biofilms: An overview of bacterial adhesion activity, and control at surfaces. American Society for Microbiology News. 58: 202–207.Google Scholar
  21. Marvalin, O., L. Aleya & H. J. Hartman, 1989. Coupling of the seasonal pattern of bacte rioplankton and phytoplankton in a eutrophic lake. Can. J. Microbiol. 35: 706–712.CrossRefGoogle Scholar
  22. Miller, T. D., 1991. Influence of inorganic and organic nutrient enrichment on blue-green algal activity and relative biomass in a eutrophic southwest Montana reservoir. Ms. Thesis, Montana State University, Bozeman, Montana. pp. 115.Google Scholar
  23. Murry, R. E., K. E. Cooksey & J. C. Priscu, 1986. Stimulation of bacterial DNA synthesis by algal exudates in attached algal-bacterial consortia. Appl. Environ. Microbiol. 52: 1177–1182.Google Scholar
  24. Nalewajko, C., 1977. Extracellular release in freshwater algae and bacteria: extracellular products of algae as a source of carbon for heterotrophs. In J. Cairns (ed.), Aquatic Microbial Communities. Garland Publ., New York: 589–624.Google Scholar
  25. Neter, J., W. Wasserman & M. H. Kutner, 1985. Applied linear statistical models. IRWIN, Homewood, Illinois: 798–823.Google Scholar
  26. Paerl, H. W., 1976. Specific association of the blue-green algaeAnabaena andAphani zomenon with bacteria in freshwater bloom. J. Phycol. 12: 431–435.CrossRefGoogle Scholar
  27. Paerl, H. W., 1978. Role of heterotrophic bacteria in promoting N2 fixation byAnabaena in aquatic habitats. Microbial Ecology 4: 215–231.CrossRefGoogle Scholar
  28. Paerl, H. W. & P. E. Kellar, 1978. Significance of bacterial-Anabaena (Cyanophyceae) associations with respect to N2 fixation in freshwater. J. Phycol. 14: 254–260.CrossRefGoogle Scholar
  29. Peterson, B. J., J. E. Hobbie, A. E. Hershey, M. A. Lock, T. E. Ford, R. M. Ventullo & G. S. Volk, 1985. Transformation of a tundra river from heterotrophy to autotrophy by the addition of phosphorus. Science 229: 1382–1386.Google Scholar
  30. Riemann, B. & M. Sondergaard, 1984. Bacterial growth in relation to phytoplankton primary production and extracellular release of organic carbon. In J. E. Hobbie & P. B. Williams (eds), Heterotrophic Activity in the Sea. NATO Publ., New York and London: 233–248.Google Scholar
  31. Riemann, B. & M. Sondergaard, 1986. Carbon dynamics in eutrophic, temperrate lakes. Elsevier Science, The Netherlands. 284 pp.Google Scholar
  32. Robarts, R. D. & R. J. Wicks, 1990. Heterotrophic bacterial production and its dependence on autotrophic production in a hypertrophic African reservoir. Can. J. Fish. Aquat. Sci. 47: 1027–1037.CrossRefGoogle Scholar
  33. Sartory, D. P. & J. U. Grobbelaar, 1984. Extraction of chlorophylla from freshwater phyto plankton for spectrophotometric analysis. Hydrobiologia 114: 177–187.Google Scholar
  34. Sundh, I., 1989. Characterization of phytoplankton extracellular products (PDOC) and their subsequent uptake by heterotrophic organisms in a mesotrophic forest lake. J. Plankton Res. 11: 463–486.Google Scholar
  35. White, P. A., J. Kalff, J. B. Rasmussen & J. M. Gasol, 1991. The effect of temperature and algal biomass on bacterial production and specific growth rate in freshwater and marine habitats. Microb. Ecol. 21: 99–118.Google Scholar
  36. Wiebe, W. J. & D. F. Smith, 1977. Direct measurement of dissolved organic carbon released by phytoplankton and incorporation by microheterotrophs. Mar. Biol. 42: 213–223.CrossRefGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1994

Authors and Affiliations

  • Lizhu Wang
    • 1
  • John C. Priscu
    • 1
  1. 1.Department of BiologyMontana State UniversityBozemanUSA

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