Fish Physiology and Biochemistry

, Volume 8, Issue 6, pp 485–495

Proteins of seminal fluid and spermatozoa in the trout (Oncorhynchus mykiss): Partial characterization and variations

  • M. Loir
  • C. Labbé
  • G. Maisse
  • A. Pinson
  • G. Boulard
  • B. Mourot
  • F. Chambeyron
Article

Abstract

The protein composition of seminal fluid, blood serum, sperm plasma membrane and flagellum of rainbow trout were analysed by SDS-polyacrylamide gel electrophoresis. Immunological identity between proteins of the 2 fluids and sperm components was studied using crossed immunoelectrophoresis, rocket immunoelectrophoresis and immunoblotting. Results indicate that many seminal proteins are antigenically-related to serum proteins, proteins of sperm origin are present in seminal fluid in varying amounts, depending on the animals and sampling time, and several serum-like seminal proteins are bound to spermatozoa.

Lipoproteins were isolated from seminal fluid (mean level: 33 μg/ml) and characterized. They were identified as being HDL-like lipoproteins. A possible physiological role is proposed for these seminal lipoproteins.

Keywords

salmonid sperm seminal fluid membrane protein electrophoresis immunoblot lipoprotein 

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Copyright information

© Kugler Publications 1990

Authors and Affiliations

  • M. Loir
    • 1
  • C. Labbé
    • 1
  • G. Maisse
    • 1
  • A. Pinson
    • 1
  • G. Boulard
    • 1
  • B. Mourot
    • 1
  • F. Chambeyron
    • 1
  1. 1.Physiologie des PoissonsINRA, Campus de BeaulieuRennesFrance

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