Skip to main content
SpringerLink
Log in

Evaluating surface drying and re-drying for wheat seed priming with polyamines: effects on emergence, early seedling growth and starch metabolism

  • Original Paper
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

This study was conducted to investigate the benefits associated with re-drying after seed priming with polyamines. Wheat (cv. AS-2002) seeds were soaked in 10 and 20 mg L−1 aerated solutions of spermidine (Spd), putrescine (Put) and spermine (Spm), and distilled water (CK2) for 12 h at 28 ± 2°C. Untreated seeds (CK1) and priming in distilled water (CK2) were taken as control treatments. Seeds were primed in two sets: In one set, after each treatment, seeds were given three surface washings with distilled water and dried closer to original moisture; in the other, seeds were only surface dried and used immediately. Use of surface-dried seeds after priming was more effective since it reduced emergence time and synchronized the emergence. Moreover, final emergence, shoot and root length, seedling fresh and dry weight were also improved. Improved starch metabolism was considered possible reason of seed invigoration. All the seed treatments resulted in a lower electrical conductivity of seed leachates compared with control; however, there was more decrease in seeds re-dried after priming than the seeds surface dried after priming. Although the effect of all the polyamines was stimulatory, Spd was the more effective for most of the attributes studied. Nonetheless, Put was more effective for seedling fresh and dry weights. All the polyamines were more effective at lower concentrations except Spm, which improved the coefficient of uniformity of emergence at high concentration. To conclude, if immediate sowing is possible, use of surface-dried seeds after priming may be more effective; seed priming with 10 mg L−1 Spd was the most effective technique when surface dried.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abdul Baki AA, Anderson JD (1970) Viability and leaching of sugars from germinating barley. Crop Sci 10:31–34

    Article  CAS  Google Scholar 

  • Afzal I, Basra SMA, Hameed A, Farooq M (2006) Physiological enhancements for alleviation of salt tolerance in spring wheat. Pak J Bot 38:1649–1659

    Google Scholar 

  • Association of Official Seed Analysists (1983) Seed vigor testing handbook. Contribution no. 32 to the handbook on seed testing. Association of Official Seed Analysis. Springfield

  • Association of Official Seed Analysists (1990) Rules for testing seeds. J Seed Technol 12:1–112

    Google Scholar 

  • Basra SMA, Farooq M, Tabassum R, Ahmad N (2006) Evaluation of seed vigor enhancement techniques on physiological and biochemical basis in coarse rice. Seed Sci Technol 34:741–750

    Google Scholar 

  • Bernfeld P (1955) Amylases α and β. Methods Enzymol 1:149–158

    Article  CAS  Google Scholar 

  • Bewley JD, Black M (1994) Seeds: physiology of development and germination. Plenum Press, New York

    Google Scholar 

  • Bouchereau A, Aziz A, Larher F, Tanguy M (1999) Polyamines and environmental challenges: recent development. Plant Sci 140:103–125

    Article  CAS  Google Scholar 

  • Bradford KJ (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. Hortic Sci 21:1105–1112

    Google Scholar 

  • Chiu KY, Chen CL, Sung JM (2002) Effect of priming temperature on storability of primed sh-2 sweet corn seed. Crop Sci 42:1996–2003

    Article  CAS  Google Scholar 

  • Chivasa W, Harris D, Chiduza C, Nyamudeza X, Mashingaidze AB (1999) Agronomic practices, major crops and farmers’ perceptions of the importance of good stand establishment in Musikavanhu communal area, Zimbabwe. J Appl Sci South Africa 4:9–25

    Google Scholar 

  • Cvikrova M, Binarova P, Cenklova V, Edera J, Machaćkova I (1999) Reinitiation of cell division and polyamine and aromatic monoamine levels in alfalfa explants during the induction of somatic embryogenesis. Physiol Plant 105:330–337

    Article  CAS  Google Scholar 

  • Dubois M, Giles KA, Hamilton JK, Roberes PA, Smith F (1956) Colorometric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Ellis RA, Roberts EH (1981) The quantification of ageing and survival in orthodox seeds. Seed Sci Technol 9:373–409

    Google Scholar 

  • Farooq M, Basra SMA, Hafeez K, Ahmad N (2005) Thermal hardening: a new seed vigor enhancement tool in rice. J Integr Plant Biol 47:187–193

    Article  Google Scholar 

  • Farooq M, Basra SMA, Afzal I, Khaliq A (2006a) Optimization of hydropriming techniques for rice seed invigoration. Seed Sci Technol 34:507–512

    Google Scholar 

  • Farooq M, Basra SMA, Hafeez K (2006b) Seed invigoration by osmohardening in fine and coarse rice. Seed Sci Technol 34:181–187

    Google Scholar 

  • Farooq M, Basra SMA, Khalid M, Tabassum R, Mehmood T (2006c) Nutrient homeostasis, reserves metabolism and seedling vigor as affected by seed priming in coarse rice. Can J Bot 84:1196–1202

    Article  CAS  Google Scholar 

  • Farooq M, Basra SMA, Tabassum R, Afzal I (2006d) Enhancing the performance of direct seeded fine rice by seed priming. Plant Prod Sci 9:446–456

    Article  Google Scholar 

  • Farooq M, Basra SMA, Hussain M, Rehman H, Saleem BA (2007) Incorporation of polyamines in the priming media enhances the germination and early seedling growth in hybrid sunflower (Helianthus annus L.). Int J Agric Biol 9:868–872

    CAS  Google Scholar 

  • Farooq M, Basra SMA, Rehman H, Hussain M (2008a) Seed priming with polyamines improves the germination and early seedling growth in fine rice. J New Seeds 9:145–155

    Article  Google Scholar 

  • Farooq M, Basra SMA, Rehman H, Saleem BA (2008b) Seed priming enhances the performance of late sown wheat (Triticum aestivum L.) by improving chilling tolerance. J Agron Crop Sci 194:55–60

    Article  Google Scholar 

  • Farooq M, Wahid A, Asad SA, Ahmad N (2009a) Comparative efficacy of surface drying and re-drying seed priming in rice: changes in emergence, seedling growth and associated metabolic events. Paddy Water Environ 8:15–22

    Article  Google Scholar 

  • Farooq M, Basra SMA, Wahid A, Khaliq A, Kobayashi N (2009b) Rice seed invigoration. In: Lichtfouse E (ed) Sustainable agriculture reviews—book series. Springer, The Netherlands

    Google Scholar 

  • Farooq M, Wahid A, Lee D-J (2009c) Exogenously applied polyamines increase drought tolerance of rice by improving leaf water status, photosynthesis and membrane properties. Acta Physiol Plant 31:937–945

    Article  CAS  Google Scholar 

  • Galston AW (1983) Polyamines as modulators of plant development. BioScience 36:382–388

    Article  Google Scholar 

  • Giri GS, Schillinger WF (2003) Seed priming winter wheat for germination, emergence and yield. Crop Sci 43:2135–2141

    Article  Google Scholar 

  • Harris D, Tripathi RS, Joshi A (2000) On-farm seed priming to improve crop establishment and yield in dry direct-seeded rice. In: Proceedings of international workshop on dry-seeded rice technology, 25–28 January, Bangkok, Thailand

  • Harris D, Tripathi RS, Joshi A (2002) On-farm seed priming to improve crop establishment and yield in dry direct-seeded. In: Pandey S, Mortimer M, Wade L, Tuong TP, Lopes K, Hardy B (eds) Direct seeding: research strategies and opportunities. International Research Institute, Manila, pp 231–240

  • He L, Nada K, Tachibana S (2002) Effects of spermidine pretreatment through the roots on growth and photosynthesis of chilled cucumber plants (Cucumis sativus L.). J Jpn Soc Hortic Sci 71:490–498

    Article  CAS  Google Scholar 

  • Huang H, Villanueva VR (1992) Amino acids, polyamines and proteins during seed germination of two species of Dipterocarpaceae. Trees Struct Funct 7:189–193

    Google Scholar 

  • Iqbal M, Ashraf M (2005) Changes in growth photosynthetic capacity and ionic relations in spring wheat (Triticum aestivum L.) due to pre-sowing seed treatments with polyamines. Plant Growth Regul 46:19–30

    Article  CAS  Google Scholar 

  • Lee SS, Kim JH (2000) Total sugars, a-amylase activity, and emergence after priming of normal and aged rice seeds. Korean J Crop Sci 45:108–111

    Google Scholar 

  • McDonald MB (2000) Seed priming. In: Black M, Bewley JD (eds) Seed technology and its biological basis. Sheffield Academic Press, Sheffield

    Google Scholar 

  • Mirza JI, Bagni N (1991) Effects of exogenous polyamines and difluoromethylornithine on seed germination and root growth of Arabidopsis thaliana. Plant Growth Regul 10:163–168

    Article  CAS  Google Scholar 

  • Naeem MA, Muhammad S (2006) Effect of seed priming on growth of barley (Hordeum vulgare) by using brackish water in salt affected soils. Pak J Bot 38:613–622

    Google Scholar 

  • Sińska I, Lewandowska U (1991) Polyamines and ethylene in the removal of embryonal dormancy in apple seeds. Physiol Plant 81:59–64

    Article  Google Scholar 

  • Srivastava LM (2002) Plant growth and development: hormones and environment. Academic Press, London

    Google Scholar 

  • Sung FJ, Chang YH (1993) Biochemical activities associated with priming of sweet corn seeds to improve vigor. Seed Sci Technol 21:97–105

    Google Scholar 

  • Taylor AG, Allen PS, Bennett MA, Bradford JK, Burris JS, Misra MK (1998) Seed enhancements. Seed Sci Res 8:245–256

    Article  Google Scholar 

  • Walker MA, Roberts DR, Shih CY, Dumbroff EB (1985) Requirement for polyamines during the cell division phase of radicle emergence in seeds of Acer saccharum. Plant Cell Physiol 26:967–971

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Agronomy, University of Agriculture, Faisalabad, 38040, Pakistan

    Muhammad Farooq, Tariq Aziz, Atique ur Rehman & Sardar Alam Cheema

  2. Department of Crop Physiology, University of Agriculture, Faisalabad, 38040, Pakistan

    Hafeez ur Rehman

  3. University of Agriculture, Faisalabad Sub-campus Depalpur, Okara, Pakistan

    Tariq Aziz

Authors
  1. Muhammad Farooq
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tariq Aziz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hafeez ur Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Atique ur Rehman
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Sardar Alam Cheema
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tariq Aziz
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muhammad Farooq.

Additional information

Communicated by M. Horbowicz.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Farooq, M., Aziz, T., ur Rehman, H. et al. Evaluating surface drying and re-drying for wheat seed priming with polyamines: effects on emergence, early seedling growth and starch metabolism. Acta Physiol Plant 33, 1707–1713 (2011). https://doi.org/10.1007/s11738-010-0707-3

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11738-010-0707-3

Keywords

Search

Navigation