Skip to main content

Advertisement

SpringerLink
Log in

Bioactive nanocomposite coatings of collagen/hydroxyapatite on titanium substrates

  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Collagen/hydroxyapatite (HA) nanocomposite thin films containing 10, 20, and 30 wt.% HA were prepared on commercially pure titanium substrates by the spin coating of their homogeneous sols. All of the nanocomposite coatings having a thickness of ∼7.5 μm exhibited a uniform and dense surface, without any obvious aggregation of the HA particles. A minimum contact angle of 36.5° was obtained at 20 wt.% HA, suggesting that these coatings would exhibit the best hydrophilicity. The in vitro cellular assays revealed that the coating treatment of the Ti substrates favored the adhesion of osteoblast-like cells and significantly enhanced the cell proliferation rate. The cells on the nanocomposite coatings expressed much higher alkaline phosphatase (ALP) levels than those on the uncoated Ti substrates. Increasing the amount of HA resulted in a gradual improvement in the ALP activity. The nanocomposite coatings on Ti substrates also exhibited much better cell proliferation behaviors and osteogenic potentials than the conventional composite coatings with equivalent compositions, demonstrating the greater potential of the former as implant materials for hard tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. H. Tschernitschek, L. Borchers, W. Geurtsen, Quintessence Int. 36, 523 (2005)

    Google Scholar 

  2. A.M. Al-Mayouf, A.A. Al-Swayih, N.A. Al-Mobarak, A.S. Al-Jabab, Mater. Chem. Phys. 86, 320 (2004)

    Article  CAS  Google Scholar 

  3. E.J. Mcpherson, L.D. Dorr, T.A. Gruen, M.T. Saberi, Clin. Orthop. 315, 223 (1995)

    Google Scholar 

  4. I.S. Lee, D.H. Kim, H.E. Kim, Y.C. Jung, C.H. Han, Biomaterials 23, 609 (2002)

    Article  CAS  Google Scholar 

  5. S. Overgaard, M. Lind, H. Glerup, S. Grundvig, C. Bunger, K. Soballe, Clin. Orthop. Relat. Res. 336, 286 (1997)

    Article  Google Scholar 

  6. J.G.C. Wolke, J.P.C.M. Van Der Waerden, H.G. Schaeken, J.A. Jansen, Biomaterials 24, 2623 (2003)

    Article  CAS  Google Scholar 

  7. J.L. Ong, M. Appleford, S. Oh, Y. Yang, W.H. Chen, J.D. Bumgardner, W.O. Haggard, JOM 58, 67 (2006)

    Article  CAS  Google Scholar 

  8. X.B. Zheng, C.X. Ding, J. Therm. Spray Technnol. 9, 520 (2007)

    Google Scholar 

  9. T. Furuzono, A. Kishida, J. Tanaka, J. Mater. Sci.: Mater. Med. 15, 19 (2004)

    Article  CAS  Google Scholar 

  10. A. Afshar, M. Yousefpour, X. Yang, X. Li, B. Yang, Y. Wu, J. Chen, X. Zhang, Mater. Sci. Eng. B 128, 243 (2006)

    Article  CAS  Google Scholar 

  11. H. W. Kim, L.H. Li, E.J. Lee, S.H. Lee, H.E. Kim, J. Biomed. Mater. Res. 75A, 629 (2005)

    Article  CAS  Google Scholar 

  12. S. Rammelt, T. Illert, S. Bierbaum, D. Scharnweber, H. Zwipp, W. Schneiders, Biomaterials 27, 5561 (2006)

    Article  CAS  Google Scholar 

  13. S. Rammelt, E. Schulze, R. Bernhardt, U. Hanisch, D. Scharnweber, H. Worch, H. Zwipp, A. Biewener, J. Orthop. Res. 22, 1025 (2004)

    Article  CAS  Google Scholar 

  14. M. Morra, C. Cassinelli, L. Meda, M. Fini, G. Giavaresi, R. Giardino, Int. J. Oral Maxillofac. Implants 20, 23 (2005)

    Google Scholar 

  15. S. Li, Z. Zheng, W. Liu, J.R. De Wijin, K. De Groot, J. Biomed. Mater. Res. 40, 520 (1998)

    Article  CAS  Google Scholar 

  16. Y. Cai, C. Liang, S. Zhu, Z. Cui, X. Yang, Scripta Mater. 54, 89 (2006)

    Article  CAS  Google Scholar 

  17. K. Hu, X.J. Yang, Y.L. Cai, Z.D. Cui, Q. Wei, Surf. Coat Technol. 201, 1902 (2006)

    Article  CAS  Google Scholar 

  18. Y. Chen, A.F.T. Mak, M. Wang, J. Li, J. Biomed. Mater. Res. 77B, 315 (2006)

    Article  CAS  Google Scholar 

  19. Y. Chen, A.F.T. Mak, M. Wang, J. Li, M.S. Wong, Surf. Coat Technol. 201, 575 (2006)

    Article  CAS  Google Scholar 

  20. S. Yokota, T. Kitaoka, H. Wariishi, Appl. Surf. Sci. 253, 4208 (2007)

    Article  CAS  Google Scholar 

  21. K.H. Wu, S.Y. Lu, H.L. Chen, Langmuir 22, 8029 (2006)

    Article  CAS  Google Scholar 

  22. X. Lin, X. Li, H. Fan, X. Wen, J. Lu, X. Zhang, Mater. Lett. 58, 3569 (2004)

    Article  CAS  Google Scholar 

  23. D.D. Lee, M.J. Glimcher, J. Mol. Biol. 217, 487 (1991)

    Article  CAS  Google Scholar 

  24. A. Tampeeri, G. Celotti, F. Szontagh, E. Landi, J. Mater. Sci.: Mater. Med. 8, 29 (1997)

    Article  Google Scholar 

  25. A. Monkawa, T. Ikoma, S. Yunoki, K. Ohta, J. Tanaka, J. Nanosci. Nanotechnol. 7, 833 (2007)

    Article  CAS  Google Scholar 

  26. H.W. Kim, H.E. Kim, V. Salih, Biomaterials 26, 5221 (2005)

    Article  CAS  Google Scholar 

  27. P. Linez-Bataillon, F. Monchau, M. Bigerelle, H.F. Hildebrand, Biomol. Eng. 19, 133 (2002)

    Article  CAS  Google Scholar 

  28. J.H. Lee, G. Khang, J.W. Lee, H.B. Lee, J. Colloid Interf. Sci. 205, 323 (1998)

    Article  CAS  Google Scholar 

  29. H.W. Kim, J.C. Knowles, H.E. Kim, J. Biomed. Mater. Res. 72A, 136 (2004)

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from the Korea Health 21 R&D Project, the ministry of Health & Welfare, Republic of Korea (02-PJ3-PG6EV11-0002).

Author information

Authors and Affiliations

  1. Department of Materials Science and Engineering, Seoul National University, Seoul, 151-744, Republic of Korea

    Shu-Hua Teng, Eun-Jung Lee, Chee-Sung Park, Won-Young Choi, Du-Sik Shin & Hyoun-Ee Kim

Authors
  1. Shu-Hua Teng
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eun-Jung Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chee-Sung Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Won-Young Choi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Du-Sik Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Hyoun-Ee Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hyoun-Ee Kim.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Teng, SH., Lee, EJ., Park, CS. et al. Bioactive nanocomposite coatings of collagen/hydroxyapatite on titanium substrates. J Mater Sci: Mater Med 19, 2453–2461 (2008). https://doi.org/10.1007/s10856-008-3370-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10856-008-3370-0

Keywords

Search

Navigation