, Volume 140, Issue 1–2, pp 65–72 | Cite as

Hempseed as a nutritional resource: An overview

  • J. C. CallawayEmail author


The seed of Cannabis sativa L. has been an important source of nutrition for thousands of years in Old World cultures. Non-drug varieties of Cannabis, commonly referred to as hemp, have not been studied extensively for their nutritional potential in recent years, nor has hempseed been utilized to any great extent by the industrial processes and food markets that have developed during the 20th century. Technically a nut, hempseed typically contains over 30% oil and about 25% protein, with considerable amounts of dietary fiber, vitamins and minerals. Hempseed oil is over 80% in polyunsaturated fatty acids (PUFAs), and is an exceptionally rich source of the two essential fatty acids (EFAs) linoleic acid (18:2 omega-6) and alpha-linolenic acid (18:3 omega-3). The omega-6 to omega-3 ratio (n6/n3) in hempseed oil is normally between 2:1 and 3:1, which is considered to be optimal for human health. In addition, the biological metabolites of the two EFAs, gamma-linolenic acid (18:3 omega-6; ‘GLA’) and stearidonic acid (18:4 omega-3; ‘SDA’), are also present in hempseed oil. The two main proteins in hempseed are edestin and albumin. Both of these high-quality storage proteins are easily digested and contain nutritionally significant amounts of all essential amino acids. In addition, hempseed has exceptionally high levels of the amino acid arginine. Hempseed has been used to treat various disorders for thousands of years in traditional oriental medicine. Recent clinical trials have identified hempseed oil as a functional food, and animal feeding studies demonstrate the long-standing utility of hempseed as an important food resource.

Key words

Cannabis food hemp oil protein seed 


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  1. Anes, E., M.P. Kühnel, E. Bos, J. Moniz-Pereira, A. Habermann & G. Griffiths, 2003. Selected lipids activate phagosome actin assembly and maturation resulting in killing of pathogenic mycobacteria. Nature Cell Biol 5(9): 793–802.PubMedGoogle Scholar
  2. Barre, D.E., 2001. Potential of evening primrose, borage, black currant, and fungal oils in human health. Ann Nutr Metab 45: 47– 57.PubMedGoogle Scholar
  3. Burr, G.O. & M.M. Burr, 1929. New deficiency disease produced by the rigid exclusion of fat from the diet. J Biol Chem 82: 345– 367.Google Scholar
  4. Burr, G.O. & M.M. Burr, 1930. On the nature and role of fatty acids essential to nutrition. J Biol Chem 86: 587–621.Google Scholar
  5. Bordoni, A., P.L. Biagi, M. Masi, G. Ricci, C. Fanelli, A. Patrizi & E. Ceccolini, 1988. Evening primrose oil (Efamol) in the treatment of children with atopic dermatitis. Drugs Exp Clin Res 14(4): 291–297.PubMedGoogle Scholar
  6. Callaway, J.C., U. Schwab, I. Harvima, P. Halonen, O. Mykkänen, P. Hyvönen & T. Järvinen, 2004. Efficacy of dietary hempseed oil on plasma lipids and skin quality in patients with atopic dermatitis. J Derm Treat (submitted).Google Scholar
  7. Callaway, J.C., 2002. Hemp as food at high latitudes. J Ind Hemp 7(1): 105–117.Google Scholar
  8. Callaway, J.C., T. Tennilä & D.W. Pate, 1997a. Occurrence of “omega-3” stearidonic acid (cis-6,9,12,15-octadecatetraenoic acid) in hemp (Cannabis sativa L.) seed. J Int Hemp Assoc 3: 61–63.Google Scholar
  9. Callaway, J.C., R.A. Weeks, L.P. Raymon, H.C. Walls & W.L. Hearn, 1997b. A positive Urinalysis from hemp (Cannabis) seed oil. J Anal Toxicol 21(4): 319–320.Google Scholar
  10. Callaway, J.C. & T.T. Laakkonen, 1996. Cultivation of Cannabis oil seed varieties in Finland. J Int Hemp Assoc 3(1): 32–34.Google Scholar
  11. Callow J., L.K. Summers, H. Bradshaw & K.N. Frayn, 2002. Changes in LDL particle composition after the consumption of meals containing different amounts and types of fat. Am J Clin Nutr 76(2): 345–350.PubMedGoogle Scholar
  12. Coghlan, A., 2003. Right kind of fat gives body the edge over TB. New Scientist, August: 12.Google Scholar
  13. Darshan, S.K. & I.L. Rudolph, 2000. Effect of fatty acids of w-6 and w-3 type on human immune status and role of eicosanoids. Nutrition 16: 143–145.Google Scholar
  14. Deferne, J.L. & D.W. Pate, 1996. Hemp seed oil: A source of valuable essential fatty acids. J Int Hemp Assoc 3(1): 1–7.Google Scholar
  15. de Padua, L.S., N. Bunyaprafatsara & R.H.M.J. Lemmens (Eds.), 1999. Plant Resources of South-East Asia: Medicinal and Poisonous Plants, Vol. 1, No. 12, pp. 167–175. Backhuys Publishers, Leiden.Google Scholar
  16. Derek, J.R. & K.A. Meckling-Gill, 1999. Both (n-3) and (n-6) fatty acids stimulate wound healing in the rat intestine epithelial cell line, IEC-6. Am Soc Nutr Sci 129: 1791–1798.Google Scholar
  17. Gerster, H., 1988. Can adults adequately convert alpha-linolenic acid (18:3n-3) to eicosapentanoic acid (20:5n-3) and docosahexanoic acid (22:6n-3)? Int J Vit Nutr Res 68: 159–173.Google Scholar
  18. Grigoriev, O.V., 2002. Application of hempseed (Cannabis sativa L.) oil in the treatment of the ear, nose and throat (ENT) disorders. J Ind Hemp 7(2): 5–15.Google Scholar
  19. Hampson, A.J., M. Grimaldi, M. Lolic, D. Wink, R. Rosenthal, J. Axelrod, 2000. Neuroprotective antioxidants from marijuana. Ann N Y Acad Sci 899: 274–282.PubMedCrossRefGoogle Scholar
  20. Harbige, L.S. & B.A.C. Fisher, 2001. Dietary fatty acid modulation of mucosally-induced tolerogenic immune response. Proc Nutr Soc 60: 449–456.CrossRefPubMedGoogle Scholar
  21. Harbige, L.S., L. Layward & M.M. Morris-Downes, 2000. The protective effects of omega-6 fatty acids in experimental autoimmune encephalomyelitis (EAE) in relation to transforming growth factor-beta 1 (TGF-β 1) up-regulation and increased prostaglandin E2 (PGE2) production. Clin Exp Immunol 122: 445–452.CrossRefPubMedGoogle Scholar
  22. Horrobin, D.F., 2000. Essential fatty acid metabolism and its modification in atopic eczema. Am J Clin Nutr 71(1): 367– 72S.Google Scholar
  23. Kassirer, J.P., 1997. Federal foolishness. New Engl J Med 30: 366.Google Scholar
  24. Kriese, U., E. Schumann, W.E. Weber, M. Beyer, L. Brühl & B. Matthäus, 2004. Oil content, tocopherol composition and fatty acid patterns of the seeds of 51 Cannabis sativa L. genotypes. Euphytica 137: 339–351.Google Scholar
  25. Laakkonen, T.T. & J.C. Callaway, 1998. Update on FIN-314. J Int Hemp Assoc 5(1): 34–35.Google Scholar
  26. G. Leson, P. Pless, J.W. Roulac, (Eds.), 1999. Hemp Foods & Oils for Health, 2nd edn. Hemptech, Ltd, Sebastopol.Google Scholar
  27. Leson, G., P. Pless, F. Grotenhermen, H. Kalant & M.A. ElSohly, 2001. Evaluating the impact of hemp food consumption on workplace drug tests. J Anal Toxicol 25(8): 691–698.PubMedGoogle Scholar
  28. McPartland, M.J. & G. Geoffrey, 2004. Random queries concerning the evolution of Cannabis and coevolution with the cannabinoid receptor. In: G. Guy, R. Robson, K. Strong, B. Whittle, (Eds.), The Medicinal Use of Cannabis, pp. 71–102. Royal Society of Pharmacists, London.Google Scholar
  29. Manku, M.S., D.F. Horribin, N. Morse, V. Kyte, K. Jenkins, S. Wright & J.L. Burton, 1982. Reduced levels of prostaglandin precursors in the blood of atopic patients; defective delta-6-desaturase function as a biochemical basis for atopy. Prostaglandins Leukot Med 9(6): 615–628.PubMedGoogle Scholar
  30. Manku, M.S., D.F. Horrobin, N.L. Morse, S. Wright & J.L. Burton, 1984. Essential fatty acids in the plasma phospholipids of patients with atopic eczema. Br J Dermatol 110(6): 643–648.PubMedGoogle Scholar
  31. Mechoulam, R., 1986. Cannabinoids as therapeutic agents, pp. 1–20. CRC Press, Boca Raton.Google Scholar
  32. Mechoulam, R. & L. Hanu, 2001. The cannabinoids: An overview. Therapeutic implications in vomiting and nausea after cancer chemotherapy, in appetite promotion, in multiple sclerosis and in neuroprotection. Pain Res Manage 6(2): 67–73.Google Scholar
  33. Mechoulam, R., D. Panikashvili & E. Shohami, 2002. Cannabinoids and brain injury: Therapeutic implications. Trends Mol Med 8(2): 58–61.PubMedGoogle Scholar
  34. Mustafa, A.F., J.J. McKinnon & D.A. Christensen, 1999. The nutritive value of hemp meal for ruminants. Can J Anim Sci 79(1): 91–95.CrossRefGoogle Scholar
  35. Ngeh-Ngwainbi, J., J. Lin & A. Chandler, 1997. Determination of total, saturated, unsaturated, and monounsaturated fats in cereal products by acid hydrolysis and capillary gas chromatography: Collaborative study. J AOAC Int 80(2): 359–372.PubMedGoogle Scholar
  36. Odani, S. & S. Odani, 1998. Isolation and primary structure of a methionine and cystine-rich seed protein of Cannabis sativa L. Biosci Biotechnol Biochem, 62: 650–654.PubMedGoogle Scholar
  37. Okuyama, H., T. Kobayashi & S. Watanabe, 1997. Dietary fatty acids- the N-6/N-3 balance and chronic elderly diseases. Excess linoleic acid and relative N-3 deficiency syndrome seen in Japan. Prog Lipid Res 3: 409–457.Google Scholar
  38. Oliwiecki, S., J.L. Burton, K. Elles & D.F. Horrobin, 1991. Levels of essential and other fatty acids in plasma red cell phospholipids from normal controls and patients with atopic eczema. Acta Derm Venereol 71(3): 224–228.PubMedGoogle Scholar
  39. Pringle, H., 1997. Ice age community may be earliest known net hunters. Science 277: 1203–1204.Google Scholar
  40. Russell, D., 2003. Phagosomes, fatty acids and tuberculosis. Nature Cell Bio 5(9): 776–778.Google Scholar
  41. Sakai, K., H. Okuyama, H. Shimazaki, M. Katagiri, S. Torii, T. Matsushita & S. Baba, 1994. Fatty acid compositions of plasma lipids in atopic dermatitis/asthma patients. Arerugi 43(1): 37– 43.PubMedGoogle Scholar
  42. H. Scherz, G. Kloos, F. Senser, (Eds.), 1986. Food Composition and Nutrition Tables 1986/1987, 3rd edn. Wissenschaftliche Veriagsgesellschaft mbH, Stuttgart.Google Scholar
  43. Silversides, F.G., K.L. Budgell & M.R. Lefrançois, 2002. Effect of feeding hemp seed meal to laying hens. Atlantic Poultry Research Institute; APRI Factsheet 16: 2 pp.Google Scholar
  44. Simopoulos, A.P., 1991. Omega-3 fatty acids in health and disease and in growth and development. Am J Clin Nutr 54: 438–463.PubMedGoogle Scholar
  45. Simopoulos, A.P., 1999. Essential fatty acids in health and chronic disease. Am J Clin Nutr 70: 560–569.Google Scholar
  46. Simopoulos, A.P., A. Leaf & N. Salem, 2000. Workshop statement on the essentiality of and recommended dietary intakes from omega-6 and omega-3 fatty acids. Prostaglandins Leukot Essent Fatty Acids 63(3): 119–121.PubMedGoogle Scholar
  47. Simopoulos, A.P., 2002a. The importance of the omega-6/omega-3 essential fatty acids. Biomed Pharmacother 56(8): 365–379.Google Scholar
  48. Simopoulos, A.P., 2002b. Omega-3 fatty acids in inflammation and autoimmune disease. J Am Coll Nutr 21(6): 495–505.Google Scholar
  49. Sirek, J., 1955. Vyznam konopného semence therapii tuberkulosy (Hempseed in the treatment of tuberculosis). Acta Universitatis Palackianae Olomucensis 6: 1–13.Google Scholar
  50. Small, E. & D. Marcus, 2003. Tetrahydrocannabinol levels in hemp (Cannabis sativa) germplasm resources. Econ Bot 57(4): 545–558.Google Scholar
  51. Takwale A., E. Tan, S. Agarwall, G. Barclay, I. Ahmed, K. Hotchkiss, J.R. Thompson, T. Chapman & J. Berth-Jones, 2003. Efficacy and tolerability of borage oil in adults and children with atopic eczema: Randomised, double blind, placebo controlled, parallel group trial. Br Med J 327(7428): 1358–1359.Google Scholar
  52. van Gool C.J., C. Thijs, C.J. Henquet, A.D. van Houwelingen, P.C. Dagnelie, J. Schrander, P.P. Menheere & P.A. van den brandt, 2003. gamma-Linolenic acid supplementation for prophylaxis of atopic dermatitis- a randomized controlled trial in infants at high familial risk. Am J Clin Nutr 77: 943–951.PubMedGoogle Scholar
  53. Whitaker, D.K., J. Chilliers & C. de Beer, 1996. Evening primrose (Epogam) in the treatment of chronic hand dermatitis; disappointing therapeutic results. Dermatology 193(2): 115–120.PubMedCrossRefGoogle Scholar
  54. WHO & FAO Joint Expert Consultation Report, 1995: Fats and oils in human nutrition. Nutr Rev 53(7): 202–205.Google Scholar
  55. Wu, D., M. Meydani, L.S. Leka, Z. Nightingale, G.J. Handelman, J.B. Blumberg & S.N. Meydani, 1999. Effect of dietary supplementation with black currant seed oil on the immune response of healthy elderly subjects. Am J Clin Nutr 70: 536–543.PubMedGoogle Scholar
  56. Xiaozhai, L. & R.C. Clarke, 1995. The cultivation and use of hemp (Cannabis sativa L.) in ancient China. J Int Hemp Assoc 2(1): 26–33.Google Scholar
  57. Yu, G. & B. Björkstén, 1998. Polyunsaturated fatty acids in school children in relation to allergy and serum IgE levels. Pediatr Allergy Immunol 9: 133–138.PubMedGoogle Scholar
  58. Weiss, E., W. Wetterstrom, D. Nadel, O. Bar-Yosef, 2004. The broad spectrum revisted: Evidence from plant remains. Proc Natl Acad Sci USA 101(26): 9551–9555.PubMedGoogle Scholar
  59. Zias, J., H. Stark, J. Sellgman, R. Levy, E. Werker, A. Breuer & R. Mechoulam, 1993. Early medical use of cannabis. Nature 363(6426): 215.PubMedGoogle Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Department of Pharmaceutical ChemistryUniversity of KuopioKuopioFinland

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