Abstract
Animal models for gastric ulcers produced by physical, pharmacological and surgical methods have been widely employed to evaluate therapeutic drugs and investigate the mechanism of action of this disease. ICR mice were selected to produce this model, even though several mice and rats have been widely used in studies of gastric ulcers. To compare the responses of ICR mice obtained from three different sources to gastric ulcer inducers, alterations in gastric injury, histopathological structure, and inflammation were measured in Korl:ICR (Korea NIFDS source), A:ICR (USA source) and B:ICR (Japan source) treated with three concentrations of ethanol (EtOH) (50, 70, and 90%) in 150 mM hydrochloric acid (HCl) solution. Firstly, the stomach lesion index gradually increased as the EtOH concentration increased in three ICR groups. Moreover, a significant increase in the level of mucosal injury, edema and the number of inflammatory cells was similarly detected in the EtOH/HCl treated group compared with the vehicle treated group in three ICR groups. Furthermore, the number of infiltrated mast cells and IL-1β expression were very similar in the ICR group derived from three different sources, although some differences in IL- 1β expression were detected. Especially, the level of IL-1β mRNA in 50 and 90EtOH/HCl treated group was higher in Korl:ICR and A:ICR than B:ICR. Overall, the results of this study suggest that Korl:ICR, A:ICR and B:ICR derived from different sources have an overall similar response to gastric ulcer induced by EtOH/HCl administration, although there were some differences in the magnitude of their responses.
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Viana AF, Fernandes HB, Silva FV, Oliveira IS, Freitas FF, Machado FD, Costa CL, Arcanjo DD, Chaves MH, Oliveira FA, Oliveira RC. Gastroprotective activity of Cenostigma macrophyllum Tul. var. acuminata Teles Freire leaves on experimental ulcer models. J Ethnopharmacol 2013; 150(1): 316–323.
Yamamoto S, Watabe K, Araki H, Kamada Y, Kato M, Kizu T, Kiso S, Tsutsui S, Tsujii M, Kihara S, Funahashi T, Shimomura I, Hayashi N, Takehara T. Protective role of adiponectin against ethanol-induced gastric injury in mice. Am J Physiol Gastrointest Liver Physiol 2012; 302(8): G773-G780.
Wang J, Zhang T, Zhu L, Ma C, Wang S. Anti-ulcerogenic effect of Zuojin Pill against ethanol-induced acute gastric lesion in animal models. J Ethnopharmacol 2015; 173: 459–467.
Chen S, Zhu K, Wang R, Zhao X. Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice. Exp Ther Med 2014; 8(1):316-322.
Yang Y, Lee J, Rhee MH, Yu T, Baek KS, Sung NY, Kim Y, Yoon K, Kim JH, Kwak YS, Hong S, Kim JH, Cho JY. Molecular mechanism of protopanaxadiol saponin fraction-mediated antiinflammatory actions. J Ginseng Res 2015; 39(1): 61–68.
Byun JS. Protective effects of Coptidis Rhizoma on ethanolinduced gastric ulcer in mice. Korean J Oriental Physiology & Pathology 2012; 26(1): 67–73.
Kim JS, Lim SW. The effects of Sasammaickmoondong-tang against gastric mucosal lesions. J Korean Orient Med 2003; 24(2): 121–137.
Adinortey MB, Ansah C, Galyuon I, Nyarko A. In Vivo models used for evaluation of potential antigastroduodenal ulcer agents. Ulcers 2013; 2013: 12.
Brzozowski T, Konturek PC, Konturek SJ, Kwiecién S, Pajdo R, Brzozowska I, Hahn EG. Involvement of endogenous cholecystokinin and somatostatin in gastroprotection induced by intraduodenal fat. J Clin Gastroenterol 1998; 27(1): 125–137.
Nam DE, Kim OK, Shim TJ, Lee JK, Hwang KT. Inhibitory effects of Chios Mastic Gum on gastric acid secretion by histamine-related pathway in a rat model and primary parietal cells. J Korean Soc Food Sci Nutr 2014; 43(10): 1500–1509.
Kazumori H, Ishihara S, Fukuda R, Kinoshita Y. Time-course changes of ECL cell markers in acetic acid-induced gastric ulcers in rats. Aliment Pharmacol Ther 2002; 16(2):10-19.
Liu J, Wang F, Luo H, Liu A, Li K, Li C, Jiang Y. Protective effect of butyrate against ethanol-induced gastric ulcers in mice by promoting the anti-inflammatory, anti-oxidant and mucosal defense mechanisms. Int Immunopharmacol 2016; 30:179–187.
Robert A, Nezamis JE, Lancaster C, Hanchar AJ. Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 1979; 77(3): 433–443.
Oates PJ, Hakkinen JP. Studies on the mechanism of ethanolinduced gastric damage in rats. Gastroenterology 1998; 94(1): 10–21.
Ku SK, Seo BI, Park JH, Park GY, Seo YB, Kim JS, Lee HS, Roh SS. Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity. World J Gastroenterol 2009; 15(38): 4799–4805.
Laine L, Weinstein WM. Histology of alcoholic hemorrhagic “gastritis”: a prospective evaluation. Gastroenterology 1988; 94(6): 1254–1262.
Prussin C, Metcalfe DD. 4. IgE, mast cells, basophils, and eosinophils. J Allergy Clin Immunol 2003; 111(2 suppl): 486–494.
Anderson DC, Kodukula K. Biomarkers in pharmacology and drug discovery. Biochem Pharmacol 2014; 87(1): 172–188.
Bueters T, Ploeger BA, Visser SA. The virtue of translational PKPD modeling in drug discovery: selecting the right clinical candidate while sparing animal lives. Drug Discov Today 2013; 18(17-18): 853–862.
Fan J, de Lannoy IA. Pharmacokinetics. Biochem Pharmacol 2014; 87(1): 93–120.
McGonigle P, Ruggeri B. Animal models of human disease: challenges in enabling translation. Biochem Pharmacol 2014; 87(1): 162–171.
Jiminez JA, Uwiera TC, Douglas Inglis G, Uwiera RR. Animal models to study acute and chronic intestinal inflammation in mammals. Gut Pathog 2015; 7: 29.
Watanabe K. Prospects for future development in the pharmacology of gastric ulcer models and of gastric acid secretion in experimental animals. Nihon Yakurigaku Zasshi 1999; 114(3): 141–148.
Fox JG, Anderson LC, Loew FM, Quimby FW. Laboratory animal medicine, 2nd ed, Academic press, New York, 2002.
Lynch CJ. The so-called Swiss mouse. Lab Anim Care 1969; 19(2): 214–220.
Chia R, Achilli F, Festing MF, Fisher EM. The origins and uses of mouse outbred stocks. Nat Genet 2005; 37(11): 1181–1186.
Cui S, Chesson C, Hope R. Genetic variation within and between strains of outbred Swiss mice. Lab Anim 1993; 27(2): 116–123.
Lehoczky JA, Cai WW, Douglas JA, Moran JL, Beier DR, Innis JW. Description and genetic mapping of Polypodia: an X-linked dominant mouse mutant with ectopic caudal limbs and other malformations. Mamm Genome 2006; 17(9): 903–913.
Jalilzadeh-Amin G, Najarnezhad V, Anassori E, Mostafavi M, Keshipour H. Antiulcer properties of Glycyrrhiza glabra L. extract on experimental models of gastric ulcer in mice. Iran J Pharm Res 2015; 14(4): 1163–1170.
Martins JL, Rodrigues OR, da Silva DM, Galdino PM, de Paula JR, Romão W, da Costa HB, Vaz BG, Ghedini PC, Costa EA. Mechanisms involved in the gastroprotective activity of Celtis iguanaea (Jacq.) Sargent on gastric lesions in mice. J Ethnopharmacol 2014; 155(3): 1616–1624.
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Song, S.H., Kim, J.E., Go, J. et al. Comparison of the response using ICR mice derived from three different sources to ethanol/hydrochloric acid-induced gastric injury. Lab Anim Res 32, 56–64 (2016). https://doi.org/10.5625/lar.2016.32.1.56
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DOI: https://doi.org/10.5625/lar.2016.32.1.56