Abstract
The present study aimed to evaluate the morphological effects of proton beam irradiation on the seed germination, seedling survival, and plant growth of soybean. Seeds of three Korean elite cultivars (Kwangankong, Daepungkong, and Pungsannamulkong) were irradiated with a 57-MeV proton beam in the range of 50 - 400 Gy. The germination rates of all the varieties increased to > 95%; however, the survival rates were significantly reduced. At doses of > 300 Gy irradiation, the Daepungkong, Kwangankong, and Pungsannamulkong cultivars exhibited 39, 75, and 71% survival rates, respectively. In addition, plant height and the fresh weight of shoots and roots were significantly decreased by doses of > 100 Gy irradiation, as were the dry weights of the shoots and roots. However, SPAD values increased with increasing doses of irradiation. Abnormal plants with atypically branched stems, modified leaves, and chlorophyll mutations were observed. Based on the survival rate, plant growth inhibition, and mutation frequency, it appears that the optimum dosage of proton beam irradiation for soybean mutation breeding is between 250 and 300 Gy.
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References
S. Rochfort and J. Panozzo, Journal of Agricultural and Food Chemistry 55, 7981 (2007).
I. Joel and Z. Guo-Nong, Food Reviews International 24, 252 (2008).
G. Y. Lee et al. Journal of the Korean Society of International Agruculture 27, 348 (2015).
T-Y. Hwang et al., Korean Journal of Breeding Science 44, 258 (2012).
H. Ulukan, Acta Agriculturae Scandinavica, Section B-Soil & Plant Science 61, 97 (2011).
P. Sikora et al., International Journal of Plant Genomics 2011, 13 (2011).
K. Shirasawa et al., Plant Biotechnology Journal 14, 51 (2016).
J. Cooper et al., BMC Plant Biology 8, 9 (2008).
T. Anai, Breeding Science 61, 462 (2012).
J. Park et al., Crop Science 56, 385 (2016).
Y. Reinprecht et al., Plant Breeding 128, 253 (2009).
Z. Cheng et al., Molecular BioSystems 10, 795 (2014).
R. Morita et al., Genes & Genetic Systems 84, 361 (2009).
K. J. Lee et al., Molecular Genetics and Genomics 291, 1561 (2016).
B. K. Ha et al., Plant Genetic Resources-Characterization and Utilization 12, S54 (2014).
A. Tanaka et al., Journal of Radiation Research 51, 223 (2010).
Y. M. Lee et al., Hortic Environ Biotechnol 56, 240 (2015).
J. Lee et al., Plant Breeding and Biotechnology 3, 384 (2015).
J. D. Lee, Korean Journal of Breeding Science 44, 29 (2012).
S. K. Suh et al., Korean Journal of Breeding Science 29, 503 (1997).
K. Y. Park et al., Korean Journal of Breeding Science 37, 111 (2005).
Y. Du et al., Mutation Research/Genetic Toxicology and Environmental Mutagenesis 759, 28 (2014).
J-S. Eun et al., Korean Journal of Horticultural Science & Technology 25, 17 (2007).
S-K. Kim et al., Journal of Crop Science and Biotechnology 15, 305 (2012).
A. Borzouei et al., Pak J Bot 42, 2281 (2010).
R. Sasikala and R. Kalaiyarasi, Electronic Journal of Plant Breeding 1, 885 (2010).
Q. Wang et al., Journal of Plant Nutrition 27, 557 (2004).
A. P. K. Ling et al., Journal of Zhejiang University Science B 14, 1132 (2013).
H. E. Jones et al., Journal of Environmental Radioactivity 74, 57 (2004).
S. Arase et al., Plant Biotechnology 28, 323 (2011).
H. Yamaguchi et al., Breeding Science 59, 169 (2009).
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Im, J., Kim, W.J., Kim, S.H. et al. Effects of proton beam irradiation on seed germination and growth of soybean (Glycine max L. Merr.). Journal of the Korean Physical Society 71, 752–757 (2017). https://doi.org/10.3938/jkps.71.752
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DOI: https://doi.org/10.3938/jkps.71.752