Skip to main content
SpringerLink
Log in

Raman spectroscopy of DNA in a wide frequency range

  • Optical Spectroscopy of Biomolecules
  • Published:
Physics of Wave Phenomena Aims and scope Submit manuscript

Abstract

A Raman spectrum of dry DNA has been recorded in a wide frequency range with a high spatial resolution. The Raman intensity of the low-frequency mode (25 cm−1) of dry DNA is found to exceed the intensity of intramolecular vibrations of nucleic bases and sugar-phosphate backbone by more than an order of magnitude. The position of the low-frequency peak changes with temperature of the DNA under study.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. P.R. Carey, Biochemical Applications of Raman and Resonanse Raman Spectroscopies (Academic Press, 1982).

    Google Scholar 

  2. S.C. Erfurth and W.L. Peticolas, “Melting and Premelting Phenomenon in DNA by Laser Raman Scattering,” Biopolymers. 14(2), 247 (1975).

    Article  Google Scholar 

  3. Ruixin Dong, Xunling Yan, Xiaofeng Pang, and Shenggang Liu, “Temperature-Dependent Raman Spectra of Collagen and DNA,” Spectrochim. Acta. A. Molecular and Biomolecular Spectroscopy. 60(3), 557 (2004).

    Article  ADS  Google Scholar 

  4. C.M. Muntean, R. Misselwitz, and H. Welfle, “The Influence of Mn2+ on DNA Structure in the Presence of Na+ Ions: A Raman Spectroscopic Study,” Spectroscopy. 20(5–6), 261 (2006).

    Article  Google Scholar 

  5. S.M. Perepelytsya and S.N. Volkov, “Low-Frequency Vibrations of DNA with Counterions in Cross-Stranded Position,” Ukr. J. Phys. 55(11), 1182 (2010).

    Google Scholar 

  6. S.M. Perepelytsya and S.N. Volkov, “Conformational Vibrations of Ionic Lattice in DNA: Manifestation in the Low-Frequency Raman Spectra,” J. Mol. Liquids. 164(1–2), 113 (2011).

    Article  Google Scholar 

  7. B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, and P. Walter, Molecular Biology of the Cell, 4th ed. (Garland Science, N.Y., 2002).

    Google Scholar 

  8. J.M. Butler, Forensic DNA Typing: Biology and Technology behind STR Markers (Academic Press, London, 2001).

    Google Scholar 

  9. J.D. Watson and F.H.C. Crick, “Molecular Structure of Nucleic Acids; a Structure for Deoxyribose Nucleic Acid,” Nature. 171(4356), 737 (1953).

    Article  ADS  Google Scholar 

  10. J.M. Berg, J.L. Tymoczko, and L. Stryer, Biochemistry, 7th ed. (Freeman, N.Y., 2012).

    Google Scholar 

  11. A. Ghosh and M. Bansal, “A Glossary of DNA Structures from A to Z,” Acta Cryst. D. 59(4), 620 (2003).

    Article  Google Scholar 

  12. P.C. Painter, L. Mosher, and C. Rhoads, “Low-Frequency Modes in the Raman Spectrum of DNA,” Biopolymers. 20(1), 243 (1981).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Lebedev Physical Institute of the Russian Academy of Sciences, Leninskiy pr. 53, Moscow, 119991, Russia

    V. S. Gorelik

  2. Bauman Moscow State Technical University, ul. 2-ya Baumanskaya 5/1, Moscow, 105005, Russia

    A. Yu. Pyatyshev

Authors
  1. V. S. Gorelik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Yu. Pyatyshev
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to V. S. Gorelik.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gorelik, V.S., Pyatyshev, A.Y. Raman spectroscopy of DNA in a wide frequency range. Phys. Wave Phen. 22, 252–254 (2014). https://doi.org/10.3103/S1541308X14040074

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S1541308X14040074

Keywords

Search

Navigation