Abstract
On the surface of the nucleosome, there are many regions for interaction with chromatin proteins, but an acidic patch, a negatively charged region formed by the residues of the histone H2A/H2B dimer, is especially prominent. The acidic patch is a target for anticancer drugs and an interaction locus for various pathogens. For instance, it was shown that this region is the binding site of the LANA protein of human gammaherpesvirus 8 and tethers the virus episomes to mitotic chromosomes. The development of methods for analyzing the binding of various compounds to nucleosomes is necessary both for understanding the mechanisms of chromatin regulation and for therapeutic agents' design. In this work, we propose a technique and measure the binding constant of the LANA protein fragment to the nucleosome. In contrast to previous studies, the measurements were carried out at a physiological salt concentration in the buffer solution. The proposed technique is based on the signal detection from the fluorescently labeled peptides after the separation of complexes of nucleosomes with peptides by gel electrophoresis. The paper also discusses mathematical models for analyzing the interaction between peptides and nucleosome and possible factors that can affect it.
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18 May 2021
An Erratum to this paper has been published: https://doi.org/10.3103/S0096392521110018
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Funding
This work was financially supported by the Russian Science Foundation (grant no. 19-74-30003).
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The study was performed without the use of animals and without involving people as subjects.
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Bondarenko ORCID http://orcid.org/0000-0002-5009-0863
Shaytan ORCID http://orcid.org/0000-0003-0312-938X
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Translated by P. Kuchina
The original online version of this article was revised:
1. Page 252, footnote Deceased is considered invalid.
2. Section ADDITIONAL INFORMATION should read as follows: R. V. Novikov and E. A. Bondarenko contributed equally to this work.
3. Section FUNDING should read as follows: This work was financially supported by the Russian Science Foundation (grant no. 19-74-30003).
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Novikov, R.V., Bondarenko, E.A., Malyuchenko, N.V. et al. Determining the Binding Constant of LANA Protein Fragment with Nucleosome. Moscow Univ. Biol.Sci. Bull. 75, 252–256 (2020). https://doi.org/10.3103/S0096392520040070
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DOI: https://doi.org/10.3103/S0096392520040070