Abstract
Phytoplankton communities can serve as bioindicators of the water system state. It is necessary to select the appropriate species of microalgae to develop a model of a natural ecosystem that will allow performing multifactor experiments on the influence of physicochemical factors on the biophysical and hydrobiological characteristics of phytoplankton. This study has allowed selecting six species from those available in a museum to establish a model algal community. We have found that similar conditions are required for their optimal growth (light, temperature, and medium nutrients' supply). A medium with low nitrogen content is proposed to be used as a basal medium. Under these conditions, the cells function in a proper way and the cultures show satisfactory growth, while the duration of reaching the stationary stage of growth (10–15 days) allows having more experiments for a limited time. The cells of the selected species have morphological differences that are sufficient for the automated identification within the polyculture. We have obtained the geometric characteristics of cells for the computer counting of each species in the community on the microphotographs.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bellinger, E.G. and Sigee, D.C., Freshwater Algae: Identification and Use as Bioindicators, Chichester: Wiley-Blackwell, 2010.
Dokulil, M.T., Algae as ecological bioindicators, in Bioindicators & Biomonitors: Principles, Concepts, and Applications, Markert, B.A., Breure, A.M., Zechmeister, H.G., Eds., Oxford: Elsevier, 2003, pp. 285–329.
Konyukhov, I.V., Selina, M.S., Morozova, T.V., and Pogosyan, S.I., Experience of continuous fluorimetric monitoring of phytoplankton at a mooring station, Oceanology, 2012, vol. 52, no. 1, pp. 130–140.
Falkowski, P.G. and Kolber, Z., Variations in chlorophyll fluorescence yields in phytoplankton in the world oceans, Plant Physiol., 1995, vol. 22, no. 2, pp. 341–355.
Buschmann, C., Photochemical and non-photochemical quenching coefficients of the chlorophyll fluorescence: Comparison of variation and limits, Photosynthetica, 1999, vol. 37, no. 2, pp. 217–224.
Beutler, M., Wiltshire, K.H., Meyer, B., Moldaenke, C., Luring, C., Meyerhofer, M., Hansen, U.-P., and Dau, H., A flurometric method for the differentiation of algal populations in vivo and in situ, Photosynth. Res., 2002, vol. 72, no. 1, pp. 39–53.
Allen, M.M., Simple conditions for growth of unicellular blue-green algae on plates, J. Phycol., 1968, vol. 4, no. 1, pp. 1–4.
Pogosyan, S.I., Gal’chuk, S.V., Kazimirko, Yu.V., Konyukhov, I.V., and Rubin, A.B., Application of the MEGA-25 fluorimeter for determining the amount of phytoplankton and assessing the state of its photosynthetic apparatus, Voda: Khim. Ekol., 2009, no. 6, pp. 34–40.
Matorin, D.N., Osipov, V.A., Yakovleva, O.V., and Pogosyan, S.I., Opredelenie sostoyaniya rastenii i vodoroslei po fluorestsentsii khlorofilla (Determination of the State of Plants and Algae by the Fluorescence of Chlorophyll), Moscow: Maks-Press, 2010.
Merzlyak, M.N. and Naqvi, K.R., On recording the true absorption and scattering spectrum of a turbid sample: Application to cell suspensions of the cyanobacterium Anabaena variabilis, J. Photochem. Photobiol., 2000, vol. 58, no. 2, pp. 123–129.
Sun, J. and Liu, D., Geometric models for calculating cell biovolume and surface area for phytoplankton, J. Plankton Res., 2003, vol. 25, no. 11, pp. 1331–1346.
Schindelin, J., Arganda-Carreras, I., Frise, E., et al., Fiji: An open-source platform for biological-image analysis, Nat. Methods, 2012, vol. 9, no. 7, pp. 676–682.
Levich, A.P., Revkova, N.V., and Bulgakov, N.G., The “consumption–growth” process in microalgae cultures and the needs of cells in mineral nutrition components, in Ekologicheskii prognoz (Ecological Forecast), Moscow: Izd. Mosk. Univ., 1986, pp. 132–139.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © P.V. Fursova, E.N. Voronova, A.P. Levich, D.V. Risnik, S.I. Pogosyan, 2017, published in Vestnik Moskovskogo Universiteta, Seriya 16: Biologiya, 2017, Vol. 72, No. 4, pp. 215–221.
About this article
Cite this article
Fursova, P.V., Voronova, E.N., Levich, A.P. et al. Selection of Species for the Laboratory-Reared Algal Community by Their Hydrobiological and Biophysical Features. Moscow Univ. Biol.Sci. Bull. 72, 184–189 (2017). https://doi.org/10.3103/S0096392517040046
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0096392517040046