Abstract
The expression of SERPINA1 gene in prostate (DU145, PC-3 and LNCaP) and liver (HepG2) tumor cell lines was studied. Alpha1-antitrypsin (AAT) level in the whole cell extracts, secretomes, subcellular fractions and SERPINA1 mRNA level in the corresponding cells were detected. Discordance between expression at these two levels in PC-3 and LNCaP lines was revealed. A new 37 kDa AAT N-terminus truncated isoform was detected in the nuclear extracts of some prostate tumor cell lines. The mechanism of 37 kDa AAT isoform intracellular retention was proposed. Two polyadenylation sites in the 3'-untranslated region of SERPINA1 transcripts were identified. A SERPINA1 gene 3'-untranslated region influence on AAT translation has been discussed.
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The article was translated by the authors.
Original Russian Text © A.A. Maslakova, M.V. Telkov, I.V. Orlovsky, O.S. Sokolova, 2015, published in Vestnik Moskovskogo Universiteta. Biologiya, 2015, No. 3, pp. 26–31.
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Maslakova, A.A., Telkov, M.V., Orlovsky, I.V. et al. Comparative analysis of SERPINA1 gene expression in tumor cell lines. Moscow Univ. Biol.Sci. Bull. 70, 127–131 (2015). https://doi.org/10.3103/S0096392515030086
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DOI: https://doi.org/10.3103/S0096392515030086