Abstract
The effect of isotonic Quinton Marine Plasma (QMP) solution on the growth and “stationary phase aging” (accumulation of “age”-related changes in cultured cells during cell proliferation slowing down within a single passage and subsequent “aging” in the stationary phase of growth) of transformed Chinese hamster cells was studied. No positive effects of QMP on the studied viability indexes of the cultured cells were found in any of the experiments. It is assumed that QMP, like many other potential anti-aging agents the authors studied recently (2,4-dinitrophenol in concentrations that provide mild uncoupling, the essential oil of oregano, hydrated C60-fullerene, etc.), can demonstrate its beneficial effect only at the level of the whole organism, triggering neurohumoral mechanisms that are not present in cytological model systems.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Yoshizawa, Y., Tanojo, H., Kim, S.J., and Maibach, H.I., Sea water or its components alter experimental irritant dermatitis in man, Skin Res. Technol., 2001, vol. 7, pp. 36–39.
Kimata, H., Tai, H., Nakagawa, K., Yokoyama, Y., Nakajima, H., and Ikegami, Y., Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patients with atopic eczema/dermatitis syndrome (AEDS), Acta Med. (Hradec Kralove, Czech Repub.), 2002, vol. 45, no. 2, pp. 83–84.
Yoshioka, S., Hamada, A., Cui, T., Yokota, J., Yamamoto, S., Kusunose, M., Miyamura, M., Kyotani, S., Kaneda, R., Tsutsui, Y., Odani, K., Odani, I., and Nishioka, Y., Pharmacological activity of deep-sea water: examination of hyperlipemia prevention and medical treatment effect, Biol. Pharm. Bull., 2003, vol. 26, no. 11, pp. 1552–1559.
Tabary, O., Muselet, C., Miesch, M.C., Yvin, J.C., Clément, A., and Jacquot, J., Reduction of chemokine IL-8 and RANTES expression in human bronchial epithelial cells by a sea-water derived saline through inhibited nuclear factor-kB activation, Biochem. Biophys. Res. Commun., 2003, vol. 309, no. 2, pp. 310–316.
Miyamura, M., Yoshioka, S., Hamada, A., Takuma, D., Yokota, J., Kusunose, M., Kyotani, S., Kawakita, H., Odani, K., Tsutsui, Y., and Nishioka, Y., Difference between deep seawater and surface seawater in the preventive effect of atherosclerosis, Biol. Pharm. Bull., 2004, vol. 27, no. 11, pp. 1784–1787.
Hataguchi, Y., Tai, H., Nakajima, H., and Kimata, H., Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome, Eur. J. Clin. Nutr., 2005, vol. 59, pp. 1093–1096.
Slapak, I., Skoupá, J., Strnad, P., and Horník, P., Efficacy of isotonic nasal wash (seawater) in the treatment and prevention of rhinitis in children, Arch. Otolaryngol. Head Neck Surg., 2008, vol. 134, no. 1, pp. 67–74.
Original Quinton (electronic resource). http://www.originalquinton.com/. Cited on September 4, 2014.
Alberola, J. and Coll, F., Marine therapy and its healing properties, Curr. Aging Sci., 2013, vol. 6, no. 1, pp. 63–75.
Martínez-Peinado, P., Maseres-Javaloy, P., Martínez-López, J.E., Carcía-Irles, M., and Sempere-Ortells, J.M., In vitro and in vivo studies to evaluate a potential activity of Quinton solution on the immune system, in International Immunology Meeting Abstracts, Oxford: Oxford Univ. Press, 2010, suppl. 1, pt. 3, p. iii93.
Khokhlov, A.N., Cell proliferation and aging, in Itogi nauki i tekhniki VINITI AN SSSR. Ser. “Obshchie problemy fizikokhimicheskoi biologii” (Advances in Science and Technology, Series General Problems of Physico-Chemical Biology), Moscow: Vseross. Inst. Nauchn. Tekh. Inform., 1988, vol. 9.
Khokhlov, A.N., Stationary cell cultures as a tool for gerontological studies, Ann. N.Y. Acad. Sci., 1992, vol. 663, pp. 475–476.
Khokhlov, A.N., Cell proliferation restriction: is it the primary cause of aging? Ann. N.Y. Acad. Sci., 1998, vol. 854, p. 519.
Khokhlov, A.N., Does aging need its own program, or is the program of development quite sufficient for it? Stationary cell cultures as a tool to search for anti-aging factors, Curr. Aging Sci., 2013, vol. 6, no. 1, pp. 14–20.
Khokhlov, A.N., Decline in regeneration during aging: appropriateness or stochastics? Russ. J. Dev. Biol., 2013, vol. 44, no. 6, pp. 336–341.
Khokhlov, A.N., Impairment of regeneration in aging: appropriateness or stochastics? Biogerontology, 2013, vol. 14, no. 6, pp. 703–708.
Khokhlov, A.N., Evolution of the term “cellular senescence” and its impact on the current cytogerontological research, Moscow Univ. Biol. Sci. Bull., 2013, vol. 68, no. 4, pp. 158–161.
Khokhlov, A.N., What will happen to molecular and cellular biomarkers of aging in case its program is canceled (provided such a program does exist)? Adv. Gerontol., 2014, vol. 4, no. 2, pp. 150–154.
Khokhlov, A.N., Klebanov, A.A., Karmushakov, A.F., Shilovsky, G.A., Nasonov, M.M., and Morgunova, G.V., Testing of geroprotectors in experiments on cell cultures: choosing the correct model system, Moscow Univ. Biol. Sci. Bull., 2014, vol. 69, no. 1, pp. 10–14.
Khokhlov, A.N., From Carrel to Hayflick and back, or what we got from the 100-year cytogerontological studies, Biophysics, 2010, vol. 55, no. 5, pp. 859–864.
Alinkina, E.S., Vorobyova, A.K., Misharina, T.A., Fatkullina, L.D., Burlakova, E.B., and Khokhlov, A.N., Cytogerontological studies of biological activity of oregano essential oil, Moscow Univ. Biol. Sci. Bull., 2012, vol. 67, no. 2, pp. 52–57.
Yablonskaya, O.I., Ryndina, T.S., Voeikov, V.L., and Khokhlov, A.N., A paradoxical effect of hydrated C60fullerence at an ultralow concentration on the viability and aging of cultured Chinese hamster cells, Moscow Univ. Biol. Sci. Bull., 2013, vol. 68, no. 2, pp. 63–68.
Khokhlov, A.N., Can cancer cells age? Stationary cell culture approach to the problem solution, in Visualizing of Senescent Cells in vitro and in vivo. Programme and Abstracts (Warsaw, Poland, December 15–16, 2012), Warsaw, 2012, pp. 48–49.
Lewis, M.R., Sea water as a medium for tissue cultures, Anat. Rec., 1916, vol. 10, no. 4, pp. 287–299.
Berges, J.A., Franklin, D.J., and Harrison, P.J., Evolution of an artificial seawater medium: improvements in enriched seawater, artificial water over the last two decades, J. Phycol., 2001, vol. 37, no. 6, pp. 1138–1145.
Khokhlov, A.N., Wei, L., Li, Y., and He, J., Teaching cytogerontology in Russia and China, Adv. Gerontol., 2012, vol. 25, no. 3, pp. 513–516.
Wei, L., Li, Y., He, J., and Khokhlov, A.N., Teaching the cell biology of aging at the Harbin Institute of Technology and Moscow State University, Moscow Univ. Biol. Sci. Bull., 2012, vol. 67, no. 1, pp. 13–16.
Khokhlov, A.N., The cell kinetics model for determination of organism biological age and for geroprotectors or geropromoters studies, in Biomarkers of Aging: Expression and Regulation. Proceeding, Licastro, F. and Caldarera, C.M., Eds., Bologna: CLUEB, 1992, pp. 209–216.
Moldaver, M.V. and Yegorov, Y.E., Sparse plating increases the heterogeneity of proliferative potential of fibroblasts, Mech. Ageing Dev., 2009, vol. 130, no. 5, pp. 337–342.
Burnaevskii, N.S., Vishnyakova, Kh.S., Safenina, A.V., Rybalko, D.V., Popov, K.V., and Egorov, E.E., Effect of oxygen partial pressure on the efficiency of colony formation and differentiation of human mesenchymal stromal cells derived from different sources, Klet. Transplantol. Tkanevaya Inzh., 2010, vol. 5, no. 4, pp. 24–30.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © A.N. Khokhlov, G.V. Morgunova, T.S. Ryndina, F. Coll, 2015, published in Vestnik Moskovskogo Universiteta. Biologiya, 2015, No. 1, pp. 9–13.
About this article
Cite this article
Khokhlov, A.N., Morgunova, G.V., Ryndina, T.S. et al. Pilot study of a potential geroprotector, “Quinton Marine Plasma,” in experiments on cultured cells. Moscow Univ. Biol.Sci. Bull. 70, 7–11 (2015). https://doi.org/10.3103/S009639251501006X
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S009639251501006X