Abstract
Using laser interference microscopy (LIM), an approach to study the state of the plasma membrane (PM) and membrane region of the neuron cytoplasm (vertebrates and invertebrates) has been developed. Changes of the state of the plasma membrane and cytoplasm of neurons at rest and modifying their functioning (using the neurotransmitter acetylcholine) were studied. A method for evaluation of the kinetics of the changes in the plasma membrane and cytoplasm of neurons in different parts of the cell has been developed. Regular processes in the plasma membrane and cytoplasm of neurons, with their frequency depending on the functional state of the neurons, have been found. It was suggested that the fluctuations depend on the changes in the relief of the plasma membrane, restructuring of the membrane cytoskeleton, and movement of intracellular vesicles in the neuron cytoplasm.
Similar content being viewed by others
References
Salanki, J., Neiro Biology of Anvertebraties, Budapest, 1973.
Monnier, A.M.S.R., Soc. Science Biol. Ses. Fial., 1933, vol. 114, pp. 1295–1297.
De Robertis, E., Science, 1967, vol. 136, p. 907.
Revin, V.V., Maksimov, G.V., and Radenovich, Ch.N., Fiziologiya i biofizika membrannykh protsessov (Physiology and Biophysics of Membrane Processes), Moscow: Mosk. Gos. Univ., 1995.
Chatterjee, S., Brindikova, T.A., Maksimov, G.V., and Revin, V.V., Eur. Biophys. J., 2000, vol. 29, nos. 4–5, p. 353.
Naciff, J.M., Behbehani, M.M., Kaetzel, M.A., and Dedman, J.R., Annexin VI modulates Ca2+ and K+ conductances of spinal cord and dorsal root ganglion neurons, Am. J. Physiol. Cell Physiol., 1996, vol. 271, pp. 2004–2015.
Tychinsky, V.P., Yagudzinsky, L.S., Leterrier, J.-F., Odensjo-Leterrier, M., and Weiss, D.G., Real-time measurements of mitochondrial activity using the dynamic phase microscope method, Eur. J. Cell Biol., 1999, nos. 78–79.
Tychinskii, V.P., Kufal’, G.E., Odintsov, A.A., and Vyshinskaya, T.V., Kvantovaya elektronika (Quantum Electronics), Moscow, 1997.
Bennet, A.H., Jupnik, H., Isterberg, H., and Richards, O.W., Phase Microscopy. Principles and Applications, New York, 1951.
Vishnyakov, G.N. and Levin, G.G., Linnik tomographic microscope for investigation of optically transparent objects, Izmerit. Tekh., 1998, no. 10, pp. 18–22.
Yusipovich, A.I., Novikov, S.M., Kazakova, T.A., Erokhova, L.A., Brazhe, N.A., Lazarev, G.L., and Maksimov, G.V., Characteristics of studying an isolated neuron by laser interference microscopy, Kvant. Elektron., 2006, no. 36, pp. 874–878.
Kamiya, N., Protoplasmic Streaming, in Protoplasmatologia Cell Biology Monographs, New York: Springer, 1959, vol. 8.
Brazhe, A.R., Brazhe, N.A., Rodionova, N.N., Yusipovich, A.I., Ignatyev, P.S., Maksimov, G.V., Mosekilde, E., and Sosnovtseva, O.V., Non-invasive study of nerve fibres using laser interference microscopy, Philos. Transact. Math. Phys. Eng. Sci., 2008, vol. 366, no. 1880, pp. 3463–3481.
Brazhe, A.R., Brazhe, N.A., Maksimov, G.V., Ignatyev, P.S., Rubin, A.B., Mosekilde, E., and Sosnovtseva, O.V., Phase-modulation laser interference microscopy: an advance in cell imaging and dynamics study, J. Biomed. Opt., 2008, vol. 13.
Brazhe, N.A., Brazhe, A.R., Pavlov, A.N., Erokhova, L.A., Yusipovich, A.I., Maksimov, G.V., Mosekilde, E., and Sosnovtseva, O.V., Unraveling cell processes: interference imaging interwoven with data analysis, J. Biol. Phys., 2006, vol. 32, nos. 3–4, pp. 191–208.
Chizh, B.A., Headley, P.M., and Paton, J.F., Coupling of sympathetic and somatic motor outflows from the spinal cord in a perfused preparation of adult mouse in vitro, J. Physiol., 1998, vol. 508, pp. 907–918.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © T.A. Kazakova, S.M. Novikov, G.G. Levin, G.V. Maksimov, 2014, published in Vestnik Moskovskogo Universiteta. Biologiya, 2014, No. 4, pp. 25–31.
About this article
Cite this article
Kazakova, T.A., Novikov, S.M., Levin, G.G. et al. Using dynamic phase microscopy for studies of the neuron cytoplasm. Moscow Univ. Biol.Sci. Bull. 69, 163–168 (2014). https://doi.org/10.3103/S0096392514040051
Received:
Published:
Issue Date:
DOI: https://doi.org/10.3103/S0096392514040051