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Expression of Nos2 and Acan Genes in Rat Knee Articular Cartilage in Osteoarthritis

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Abstract

Degenerative changes in the cartilage tissue of rats with sodium monoiodoacetate-induced osteoarthritis were detected during this histological study and the effect was evaluated of chondroitin sulfate and a multiprobiotic preparation on the healing process, such as the absence, due to the coadministration of these preparations, of postnecrotic changes in the cartilage surface and its replacement by fibrous elements. A molecular analysis of the cartilage tissue sampled from rats with experimental osteoarthritis has equally shown an increase in the Nos2 gene expression and a decrease in the Acan gene expression compared with the control group of animals, which indicates the activation of inflammatory and destructive processes in the tissue. Due to the coadministration of chondroitin sulfate and the multiprobiotic in the same conditions, the expression pattern of the Nos2 and Acan genes returned to the control values, which indicated the clinical prospects for the multiprobiotic as a substance able to enhance the anti-inflammatory and antioxidant action of this chondroprotector in osteoarthritis.

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ACKNOWLEDGMENTS

The general ethical norms for carrying out experiments in animals (according to the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, the First National Congress of Bioethics (Ukraine, September 2001), and the Meeting of the Commission for Problems of Bioethics, the National Scientific Center–Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv (protocol no. 3, September 25, 2017)) were observed during our studies in animals.

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This study was not supported by any financial sources.

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Authors and Affiliations

  1. Educational and Scientific Center–Institute of Biology and Medicine, Taras Shevchenko National University of Kyiv, Kyiv, Ukraine

    A. S. Dranitsina, K. O. Dvorshchenko, O. H. Korotkyi, A. A. Vovk, T. M. Falalyeyeva, D. M. Grebinyk & L. I. Ostapchenko

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  1. A. S. Dranitsina
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  2. K. O. Dvorshchenko
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  3. O. H. Korotkyi
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  4. A. A. Vovk
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  5. T. M. Falalyeyeva
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  6. D. M. Grebinyk
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  7. L. I. Ostapchenko
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Correspondence to A. S. Dranitsina or A. A. Vovk.

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The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.

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Translated by N. Tarasyuk

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Dranitsina, A.S., Dvorshchenko, K.O., Korotkyi, O.H. et al. Expression of Nos2 and Acan Genes in Rat Knee Articular Cartilage in Osteoarthritis. Cytol. Genet. 53, 481–488 (2019). https://doi.org/10.3103/S0095452719060021

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