Abstract—A new method of double immunofluorescence analysis by flow cytometry is developed to evaluate the quantitative expression of the mesenchymal marker vimentin in epithelium cells of a solid tumor, which in turn is a mixture of different histogenesis cells. The method has a number of evident advantages compared to the clinically used immunohistochemistry technique for marker investigation: the intralaboratory precision of the results, gentle preanalytical preparation, lack of subjectivity in the interpretation of the results, and integral evaluation of the markers expression in a large number of cells from a large volume of the tumor tissue sample (≥5000 cells from a sample of ≥2 cm in diameter).
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The work was performed as part of the research program planned at the Blokhin Russian Cancer Research Center, Moscow, Russia (registration no. 115112540012).
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Translated by M. Novikova
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Bogush, T.A., Kaliuzhny, S.A., Basharina, A.A. et al. Immunofluorescence Analysis of the Vimentin Expression in Epithelial Cells. Moscow Univ. Chem. Bull. 74, 290–295 (2019). https://doi.org/10.3103/S0027131419060063
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DOI: https://doi.org/10.3103/S0027131419060063