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Influence of Different Types of Block Copolymers on the Secondary Structure and Stability of the Staphylolytic Lysk Enzyme

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Moscow University Chemistry Bulletin Aims and scope

Abstract

A branched block copolymer of polyethyleneimine and polyethylene glycol (PEI–PEG) and a linear block copolymer of polylysine and polyethylene glycol (PLL–PEG) form complexes with the staphylolytic LysK enzyme. The complexation between LysK and cationic block-copolymers results in a 1.5–2.0-fold increase in the lytic activity of LysK and a 2–20-fold increase in its half-life. The stabilizing effects of the block copolymers depend on the temperature, the NaCl concentration, and the polymer/enzyme ratio. Being an effective stabilizer, linear PLL–PEG provides an increase in the LysK stability suitable for biomedical materials (LysK maintains 100% of its activity after four months at 4°C).

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    L. Y. Filatova, I. A. Novozhilov, T. A. Chubar, N. G. Balabushevich & N. L. Klyachko

  2. Animal Biosciences and Biotechnology Laboratory, Beltsville Agricultural Research Center, NEA, ARS, USDA, Beltsville, MD, 20705, USA

    D. M. Donovan

  3. Vector Center of Virology & Bioengineering, Novosibirsk, 630559, Russia

    V. G. Pugachev

Authors
  1. L. Y. Filatova
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  2. D. M. Donovan
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  3. I. A. Novozhilov
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  4. T. A. Chubar
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  5. N. G. Balabushevich
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  6. V. G. Pugachev
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  7. N. L. Klyachko
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Corresponding author

Correspondence to L. Y. Filatova.

Additional information

Original Russian Text © L.Y. Filatova, D.M. Donovan, I.A. Novozhilov, T.A. Chubar, N.G. Balabushevich, V.G. Pugachev, N.L. Klyachko, 2018, published in Vestnik Moskovskogo Universiteta, Seriya 2: Khimiya, 2018, No. 4, pp. 290–296.

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Filatova, L.Y., Donovan, D.M., Novozhilov, I.A. et al. Influence of Different Types of Block Copolymers on the Secondary Structure and Stability of the Staphylolytic Lysk Enzyme. Moscow Univ. Chem. Bull. 73, 179–184 (2018). https://doi.org/10.3103/S0027131418040028

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  • DOI: https://doi.org/10.3103/S0027131418040028

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