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Catalytic characteristics of enzyme-polyelectrolyte complexes based on hexahistidine-containing organophosphorus hydrolase

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Moscow University Chemistry Bulletin Aims and scope

Abstract

A number of covalent and non-covalent polyelectrolyte complexes based on enzyme such as hexahistidine-containing organophosphorus hydrolase was developed in this work. Polyanions and polycations were used in the form of block-copolymers with poly(ethylene glycol). To obtain covalent complexes of the enzyme, different coupling agents (glutaric aldehyde, N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt) were tried. The best catalytic efficiency of the action of the covalently bound complex ((2.8 ± 0.3) × 108 M−1 s−1) was obtained for samples with EDC. The modification of the protein surface was undertaken as an approach to its further biomedical application.

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Authors and Affiliations

  1. Department of Chemistry, Moscow State University, Moscow, 119991, Russia

    I. V. Lyagin, E. N. Efremenko & A. V. Kabanov

Authors
  1. I. V. Lyagin
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  2. E. N. Efremenko
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  3. A. V. Kabanov
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Correspondence to E. N. Efremenko.

Additional information

Original Russian Text © I.V. Lyagin, E.N. Efremenko, A.V. Kabanov, 2014, published in Vestnik Moskovskogo Universiteta. Khimiya, 2014, No. 3, pp. 167–173.

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Lyagin, I.V., Efremenko, E.N. & Kabanov, A.V. Catalytic characteristics of enzyme-polyelectrolyte complexes based on hexahistidine-containing organophosphorus hydrolase. Moscow Univ. Chem. Bull. 69, 125–130 (2014). https://doi.org/10.3103/S0027131414030055

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  • DOI: https://doi.org/10.3103/S0027131414030055

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