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Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing

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Abstract

For the rapid preparation of strains of live influenza vaccine, it is important to have a rapid method for screening vaccine reassortants. In this paper, a method of pyrosequencing was used to analyze the genome of reassortants obtained by crossing the donor of attenuation B/Leningrad/14/17/55 and the wild-type virus B/Indiana/25/2015. It has been shown that pyrosequencing allows the composition of the reassortant genome to be evaluated quickly, economically, and with high accuracy.

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ACKNOWLEDGMENTS

This work was supported by grant no. 14-15-00034 of the Russian Science Foundation (Moscow, Russia).

CONFLICT OF INTERESTThe authors declare no conflict of interest.

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Authors and Affiliations

  1. Institute of Experimental Medicine, 197376, St. Petersburg, Russia

    E. V. Krutikova, E. A. Stepanova, I. V. Kiseleva & L. G. Rudenko

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  1. E. V. Krutikova
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  2. E. A. Stepanova
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  3. I. V. Kiseleva
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  4. L. G. Rudenko
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Correspondence to E. V. Krutikova.

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Translated by A. Ostyak

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Krutikova, E.V., Stepanova, E.A., Kiseleva, I.V. et al. Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing. Mol. Genet. Microbiol. Virol. 33, 139–144 (2018). https://doi.org/10.3103/S089141681802009X

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  • DOI: https://doi.org/10.3103/S089141681802009X

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