Abstract
Acidification of extracellular medium in malignant tumors increases the invasive behaviors of cancer cells. In normal healthy tissues, acid production is catalyzed by carbonic anhydrases. Some of the carbonic anhydrase enzymes are overexpressed in certain types of cancer. The present study aimed to investigate the effect of acetazolamide, a potent carbonic anhydrase inhibitor, on in vitro cultivated cancer cells. Three different assays (MTT test, wound healing and clonogenic assay) were performed using human colorectal adenocarcinoma cells (SW620) to evaluate the suppressive effect of acetazolamide, on the colorectal cancer cells migration ability, colony formation and cell viability. The dose-dependent (1–1000 μM) reducing effect of acetazolamide on the cell viability was more significant within the first 48 h. This inhibitory effect of acetazolamide was found to be decreased at 72 h, and affects cells migration ability of cells at 24 and 48 h. Acetazolamide was observed to inhibit the cell viability, migration and colony formation ability of cells, depending on dose.
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Abbreviations
- ANOVA:
-
analysis of variance
- anti-VEGF:
-
anti-vascular endothelial growth factor
- AQP:
-
aquaporin
- CA:
-
carbonic anhydrase
- CO2 :
-
carbon dioxide
- EDTA:
-
ethylenediaminetetraacetic acid
- ELISA:
-
enzyme-linked immunosorbent assay
- FBS:
-
fetal bovine serum
- 5-FU:
-
5-fluorouracil
- HCO3 :
-
bicarbonate
- H2CO3 :
-
carbonic acid
- IC50 :
-
half maximal inhibitory concentration
- MTT:
-
3-[4,5-dimethylthiazol-2-yl] -2,5-diphenyltetrazolium bromide
- PBS:
-
phosphate-buffered saline
- RPMI:
-
Roswell Park Memorial Institute
- SD:
-
standard deviation
- SPSS:
-
Statistical Package for the Social Sciences
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Acknowledgments
The study was supported by Inonu University Scientific Research Projects Coordination Unit with project number 2014/29. The authors would like to thank Prof. Dr. Martin R. Berger (The German Cancer Research Center, Heidelberg, Germany). We thank the anonymous Reviewers for their valuable comments.
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Karakuş, F., Eyol, E., Yılmaz, K. et al. In vitro effect of carbonic anhydrase inhibitor acetazolamide on cell viability, migration and colony formation of colorectal cancer cells. Biologia 73, 621–628 (2018). https://doi.org/10.2478/s11756-018-0064-z
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DOI: https://doi.org/10.2478/s11756-018-0064-z