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Striped murrel S1 family serine protease: immune characterization, antibacterial property and enzyme activities

  • Section Cellular and Molecular Biology
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Abstract

We report a molecular characterization of S1 family serine protease (SP-1) from snakehead murrel (or called striped murrel) Channa striatus (Cs). CsSP-1 polypeptide contained a catalytic core domain (otherwise known as serine protease trypsin domain) between H20 and I237 along with a catalytic triad at H61, D104 and S197. Phylogenetic analysis confirmed that CsSP-1 belongs to serine protease S1 family. The tertiary structure showed that CsSP-1 contains 14 β-sheets as 2 separate β-barrels (the first β-barrel consists of 8 β-sheets in the N-terminal region and the second β-barrel consists of 6 β-sheets in the C-terminal region) and 3 α-helical regions. Significantly (P < 0.05) the highest CsSP-1 mRNA expression was observed in intestine, liver and kidney, moderate expression was seen in spleen, head kidney, skin and blood, and the lowest one in brain, gill, muscle and heart. Further, the expression was induced in intestine with fungus Aphanomyces invadans and bacteria Aeromonas hydrophila. The recombinant CsSP-1 protein showed antibacterial activity against both gram-negative and gram-positive bacteria. The optimum CsSP-1 enzyme activity against the substrate casein was determined at 8 mM casein concentration. Moreover, the activity was highly influenced by 5 mM phenyl-methylsulfonyl fluoride followed by ethylenediaminetetraacetic acid, 4-(2-aminoethyl)benzenesulfonylfluoride hydrochloride and calpain inhibitor I. The CsSP-1 enzyme exhibited the highest activity at pH 7.5 and temperature 35°C. The overall results showed the potential involvement of CsSP-1 in the immune system of murrels. However, further research is necessary to study the mechanism of implicit trypsin association in the defence process.

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Abbreviations

AEBSF HCl:

4-(2-aminoethyl)benzenesulfonylfluoride hydrochloride

ALLN:

calpain inhibitor I

CDD:

conserved domain database

CsSP-1:

Channa striatus family 1 serine protease

EDTA:

ethylenediaminetetraacetic acid

EUS:

epizootic ulcerative syndrome

IPTG:

isopropyl-β-thiogalactopyranoside

LB:

Luria-Bertani

MBP:

maltose-binding protein

ORF:

open reading frame

PBS:

phosphate buffer saline

qRT-PCR:

quantitative real-time polymerase chain reaction

SP:

serine protease

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  1. Division of Fisheries Biotechnology & Molecular Biology, Department of Biotechnology, Faculty of Science and Humanities, SRM University, Kattankulathur, 603 203, Chennai, Tamil Nadu, India

    Jesu Arockiaraj, Rajesh Palanisamy, Venkatesh Kumaresan, Prasanth Bhatt & Mukesh Kumar Chaurasia

  2. Department of Biotechnology, Faculty of Applied Sciences, AIMST University, Semeling Bedong, 08100, Bedong, Kedah, Malaysia

    Marimuthu Kasi

  3. Lab PCN 206, Microbiology Division, CSIR-Central Drug Research Institute, B.S. 10/1, Sector 10, Jankipuram Extension, Sitapur Road, Lucknow, 226 031, Uttar Pradesh, India

    Mukesh Pasupuleti

  4. Institute for Cellular and Molecular Biology, The University of Texas at Austin, 1 University Station A4800, Austin, TX, 78712, USA

    Annie J. Gnanam

Authors
  1. Jesu Arockiaraj
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  2. Rajesh Palanisamy
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  3. Venkatesh Kumaresan
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  4. Prasanth Bhatt
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  5. Mukesh Kumar Chaurasia
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  6. Marimuthu Kasi
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  7. Mukesh Pasupuleti
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  8. Annie J. Gnanam
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Correspondence to Jesu Arockiaraj.

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Arockiaraj, J., Palanisamy, R., Kumaresan, V. et al. Striped murrel S1 family serine protease: immune characterization, antibacterial property and enzyme activities. Biologia 69, 1065–1078 (2014). https://doi.org/10.2478/s11756-014-0410-8

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  • DOI: https://doi.org/10.2478/s11756-014-0410-8

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