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Genetic Diversity of Trichomonas Vaginalis Clinical Isolates According to Restriction Fragment Length Polymorphism Analysis of the 60-kDa Proteinase Gene

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Abstract

Introducction

Trichomonas vaginalis is a highly prevalent parasitic that causes the sexually transmitted disease trichomoniasis with some serious health complications. More understanding about genetic features of the parasite can be helpful in the study of the pathogenesis, epidemiology of the infection and drug susceptibility. For this end, we conducted analysis of a fragment (23 kDa) of the p60 of T. vaginalis gene.

Material and methods

The restriction fragment length polymorphism (RFLP) methods was used.

Result and discussion

RFLP analysis showed the difference between T. vaginalis isolates from symptomatic and asymptomatic patients, suggesting a relation between the genetic identity of the isolates and their clinical manifestations.

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Authors and Affiliations

  1. Parasitology Department, Institute of Tropical Medicine “Pedro Kouri”, A.P. 601. Marianao 13, Havana, Cuba

    Hilda Hernández, Jorge Fraga, Ricardo Marcet, Annia Alba, Mabel Figueredo, Yenisey Alfonso, Lázara Rojas & Jorge Sarracent

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  1. Hilda Hernández
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  2. Jorge Fraga
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  3. Ricardo Marcet
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Correspondence to Hilda Hernández.

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Hernández, H., Fraga, J., Marcet, R. et al. Genetic Diversity of Trichomonas Vaginalis Clinical Isolates According to Restriction Fragment Length Polymorphism Analysis of the 60-kDa Proteinase Gene. Acta Parasit. 64, 300–307 (2019). https://doi.org/10.2478/s11686-019-00065-5

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