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Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression

  • Research Article
  • Published:
Central European Journal of Biology

Abstract

Continuous increase in the number and the variety of anthropogenic sources of electromagnetic radiation causes a high interest in studying the effects ultrahigh frequency on living organisms. In the present research influence of UHF EMR (15 W, 2.45 GHz) for 5 and 15 min on morphological and genetic peculiarities of Photobacterium phosphoreum colonies was studied. It has been revealed that UHF EMR affected colony growth parameters, induced transcriptional activity of luciferase encoding gene expression and that the effect was depended on exposure duration. The subsequent cultivation of bacteria during a two week period after treatment showed maintaining of the increased luxb mRNA level in irradiated colonies. Opposite bacterial stress responses were detected to UHF EMR and elevated temperature treatments that assumed UHF EMR comprised of not only thermal but specific component of non-thermal nature.

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Abbreviations

Ct:

threshold cycle

qRT-PCR:

quantitative reverse transcription PCR

UHF EMR:

ultrahigh frequency electromagnetic radiation

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Authors and Affiliations

  1. Department of Physiology of Industrial Microorganisms, Zabolotny Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 03680, Kyiv, Ukraine

    Liubov Zelena, Igor Gretsky & Elena Gromozova

Authors
  1. Liubov Zelena
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  2. Igor Gretsky
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  3. Elena Gromozova
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Correspondence to Igor Gretsky.

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Zelena, L., Gretsky, I. & Gromozova, E. Influence of ultrahigh frequency irradiation on Photobacterium phosphoreum luxb gene expression. cent.eur.j.biol. 9, 1004–1010 (2014). https://doi.org/10.2478/s11535-014-0347-5

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  • DOI: https://doi.org/10.2478/s11535-014-0347-5

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