Abstract
The effects of benzo(a)pyrene (Bap) (0.03, 0.3 and 3 μg L−1) and chrysene (CHR) (0.3, 2.1 and 14.7 μg L−1) on the function of the immune system of juvenile white shrimp Litopenaeus vannamei were determined under laboratory conditions. This included the total hemocyte count (THC) in the hemolymph, phagocytic activityand pro-phenoloxidase (pro-PO) activity of the hemocyte, phenoloxidase (PO) activity, α2-macroglobulin (α2-M) activity, bacteriolytic activity and antibacterial activity in the hemolymph. The results showed that BaP and CHR could inhibit the immune function of L. vannamei significantly under high concentration BaP and CHR exposure. The results of this study indicated that the immunotoxicity of PAHs in a descending order was BaP>CHR. Moreover, the results indicated the THC in hemolymph, pro-PO activity and phagocytic activity of hemocyte, and bacteriolytic activity in hemolymphcould be used as potentially suitable biomarkersfor early warning indication of PAHs toxicity, this could provide useful information for toxic risk assessment of environmental pollutants.
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Wang, L., Pan, L.Q., Liu, N., Liu, D., Xu, C.Q., Miao, J.J., Biomarkers and bioaccumulation of clam Ruditapes philippinarumin response to combined cadmium and benzo[a]pyrene exposure, Food Chem. Toxicol., 2011, 49, 3407–3417.
US EPA, 2009. United States Environmental Protection Agency Priority Pollutants. 〈http://www.epa.gov/waterscience/methods/pollutants.htm〉 (accessed 02.04.09).
Zhou, L. M., Meng, X. H., Xiao, H., Tang, X. X., Interactive studies of three kinds of polycyclic aromatic hydrocarbonsand UV-B radiation on the growth of three speciesof red tide microalgae. J. Wuhan Univ.: Natl. Sci. Ed., 2006, 52(6), 773–777 (in Chinese with English abstract).
Livingstone, D.R., Organic xenobiotic metabolism in marine invertebrates. In: Gilles, R. (Ed.), Advances in Comparative and Environmental Physiology, vol 7. Springer-Verlag, Berlin, pp, 1991, 46–185.
Livingstone, D.R., The fate of organic xenobiotics in aquatic ecosystems:quantitative and qualitative differences in biotransformation by invertebrates and fish, Comp. Biochem. Physiol. C, 1998, 120, 43–49.
Valavanidis, A., Vlahogianni, T., Dassenakis, M., Scoullos, M., Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants, Ecotoxicol. Environ. Saf., 2006, 64, 178–189.
Galloway, T.S. and Depledge, M.H., Immunotoxicity in invertebrates: measurement and ecotoxicological relevance, Ecotoxicology, 2001, 10, 5–23.
Blaise, C., Trottier, S., Gagné, F., Lallement, C., Hansen, P.D., Immunocompetence of bivalve haemocytes as evaluated by a miniaturized phagocytosisassay. Environ. Toxicol., 2002, 17, 160–169.
Hannam, M.L., Bamber, S.D., Moody, J.A., Galloway, T.S., Jones, M.B., Effects of themodel PAH phenanthrene on immune function and oxidative stress in the haemolymph of the temperate scallop Pecten maximus, Chemosphere, 2010, 78, 779–784.
Liu, J., Pan, L.Q., Zhang, L., Miao, J.J., Wang, J., Immune responses, ROS generation and the haemocyte damage of scallop Chlamys farreri exposed to Aroclor 1254, Fish Shellfish Immun., 2009, 26, 422–428.
Zhang L., Pan L.Q., Liu J., Immunotoxicity effect of Benzo[α]Pyrene on scallop Chlamys farreri, J. Ocean Univ. China, 2008, 8(1), 89–94.
Dissanayake, A., Galloway, T.S., Jones, M.B., Nutritional status of Carcinus maenas (Crustacea: Decapoda) in fluences susceptibility to contaminant exposure, Aquat. Toxicol., 2008, 89, 40–46.
Dissanayake, A., Galloway, T.S., Jones, M.B., Physiological responses of juvenile and adult shore crabs Carcinus maenas(Crustacea: Decapoda) to pyrene exposure, Mar.Environ. Res., 2008, 66, 445–450.
Luengen, A.C., Friedman, C.S., Raimondi, P.T., Flegal, A.R., Evaluation of musselimmune responses as indicators of contamination in San Francisco Bay, Mar.Environ. Res., 2004, 57, 197–212.
Fang, W.H., Li, G.L., Zhou, S., Li, X.C., Hu, L.L., Zhou, J.F., Pharmacokinetics and Tissue Distribution of Thiamphenicol and Florfenicol in Pacific White Shrimp Litopenaeus Vannamei in Freshwater following Oral Administration, J. Aquat. Anim. Health, 2013, 25, 83–89.
Liu, C.H., Chen, J.C., Effect of ammonia on the immune response of white shrimp Litopenaeus vannameiand its susceptibility toVibrio alginolyticus. Fish Shellfish Immun., 2004, 16, 321–334.
Yang, L.B. and Pan, L. Q., Effects of phosphatidyl serine on immune response in the shrimp Litopenaeus vannamei, Cent. Eur. J. Biol., 2013, 8(11), 1135–1144.
Yue, F., Pan, L.Q., Xie, P., Zheng, D.B., Li, J., Immune responses and expression of immune-related genes in swimming crab Portunus trituberculatus exposed to elevated ambient ammonia-N stress, Comp. Biochem. Physiol. A, 2010, 157, 246–251.
Hernández-López, J., Gollas-Galván, T., Vargas-Albores, F., Activation of the prophenoloxidase system of the brown shrimp (Penaeus californiensis Holmes), Comp. Biochem. Physiol. C, 1996, 113, 61–66.
Ashida, M., Purification and characterization of prephenoloxidase from hemolymph of the silkworm Bombyx mori, Arch. Biochem. Biophys., 1971, 144, 749–762.
Armstrong, P.B., Quigley, J.P., Rickles, F.R., The Limulus blood cell secretes α2-macroglobulin when activated, Biol. Bull., 1990, 178, 137–143.
Gollas-Galván, T., Sotelo-Mundo, R.R., Yepiz-Plascencia, G., Vargas-Requena, C., Vargas-Albores, F., Purification and characterization of alpha 2-macroglobulin from the white shrimp (Penaeus vannamei), Comp. Biochem. Physiol. C, 2003, 134, 431–438.
Hultmark, D., Steiner, H., Rasmuson, T., Boman, H.G., Insect immunity. purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia. Eur, J. Biochem., 1980, 106, 7–16.
Chang, M., Wang, W.N., Wang, A.L., Tian, T.T., Wang, P., Zheng, Y., Liu, Y., Effects ofcadmium on respiratory burst, intracellular Ca2+ and DNA damage in the white shrimp Litopeneaus vannamei, Comp. Biochem. Physiol. C, 2009, 149, 581–586.
Hannam, M.L., Bamber, S.D., Sundt, R.C., Galloway, T.S., Immune modulationin the blue mussel Mytilus edulisexposed to North Sea produced water, Environ.Pollut., 2009, 157, 1939–1944.
Dissanayake, A., Piggott, B., Baldwin, C., Sloman, K.A., Elucidating cellular and behavioural effects of contaminant impact (polycyclic aromatic hydrocarbons, PAHs) in both laboratory-exposed and field-collected shore crabs, Carcinus maenas(Crustacea: Decapoda), Mar.Environ. Res., 2010, 70, 368–373.
Livingstone, D.R., Martinez, P.G., Michel, X., Narbonne, J.F., Ohara, S., Ribera, D., Winston, G.W., Oxyradical production as a pollution-mediated mechanism of toxicity in the common mussel, Mytilus edulis(L.), and other mollusks, Funct. Ecol., 1990, 4, 415–424.
Hannam, M.L., Bamber, S.D., Galloway, T.S., Moody, J.A., Jones, M.B., Functional immune response in Pecten maximus: Combined effectsof a pathogen-associated molecular pattern and PAH exposure, Fish Shellfish Immun., 2010, 28(1), 249–252.
Nelson, A., Auffret, N., Borlakoglu, J., Interaction of hydrophobic organic compounds with mercury adsorbed dileoylphosphatidycholine monolayers, ActaBioch. Bioph., 1990, 1021, 205–216.
Camus, L., Jones, M.B., Borseth, J.F., Grosvik, B.E., Regoli, F., Depledge, M.H., Total oxyradical scavenging capacity and cell membrane stability of haemocytes of the Arcticscallop, Chlamys islandicus, following benzo(a)pyreneexposure, Mar.Environ. Res., 2002, 54, 425–430.
Pan, L.Q., Ren, J.Y., and Liu, J, Responses of antioxidant systems and LPO level to benzo(a) pyreneand benzo(k)fluoranthene in the haemolymph of the scallop Chlamys Ferrari, Environ. Pollut., 2006, 141, 443–451.
Smith, V.J., Johnston, P.A., Differential haemotoxic effect of PCB congeners in the common shrimp, Crangon crangon, Comp. Biochem. Physiol. C, 1992, 101, 641–649.
Li, F.H. and Xiang, J.H., Recent advances in researches on the innate immunityof shrimp in China, Dev. Comp. Immunol., 2003, 39, 11–26.
Söderhäll, K. and Cerenius, L., Role of the prophenoloxidase-activating system in invertebrate immunity, Curr. Opin Immunol, 1998, 10(1), 23–28.
Cerenius, L., Lee, B.L., and Söderhäll, K., The proPO-system: pros and cons for its role in invertebrate immunity, Trends Immunol., 2008, 29(6): 263–71.
Teresa, G.G., Rogerio, R., Sotelo-Mundo, Yepiz-Plascencia, Vargas-Requena, C., Vargas-Albores, F., Purification and characterization of α2-macroglobulin from the white shrimp (Penaeus vannamei), Comp. Biochem. Physiol. C, 2003, 134, 431–438.
Pan, L.Q., Hu, F.W., Jing, F.T., Liu, H.J., The effect of different acclimation temperatures onthe prophenoloxidase system and other defence parameters in Litopenaeus vannamei, Fish Shellfish Immun., 2008, 25, 137–142.
Enghild, J.J., Thogersen, I.B., Salvesen, G., Fey, G.H., Figler, N.L., Gonias, S.L., Pizzo, S.V., Alphamacroglobulin from Limulus polyphemus exhibits proteinase inhibitory activity and participates in a hemolytic system. Biochemistry, 1990, 29, 10070–10080.
Li, C., Zhao, J., Song, L., Mu, C., Zhang, H., Gai, Y., Qiu, L., Yu, Y., Ni, D., Xing, K., Molecular cloning, genomic organization and functional analysis of an anti-lipopolysaccharide factor from Chinese mitten crab Eriocheir sinensis. Dev. Comp. Immunol., 2008, 32, 784–794.
Imjongjirak, C., Amparyup, P., Tassanakajon, A., Sittipraneed, S., Molecular cloningand characterization of crustin from mud crab Scylla paramamosain. Mol. Biol. Rep., 2009, 36, 841–850.
Rickwood, C.J., Galloway, T.S., Acetylcholinesterase inhibition as a biomarkerof adverse effect: a study of Mytilus edulis exposed to the priority pollutant chlorfenvinphos, Aquat. Toxicol., 2004, 67, 45–56.
Martín-Díaz, M.L., Blasco, J., Sales, D., DelValls, T.A., Field validation of a battery of biomarkers to assess sedimentquality in Spanish ports, Environ. Pollut., 2008, 1, 631–640.
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Ren, X., Pan, L. & Wang, L. Immunotoxic effect of Benzo[α]Pyrene and chrysene in juvenile white shrimp Litopenaeus vannamei . cent.eur.j.biol. 9, 1048–1057 (2014). https://doi.org/10.2478/s11535-014-0317-y
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DOI: https://doi.org/10.2478/s11535-014-0317-y