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Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions

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Abstract

This study aimed to develop a label-free, sensitive, selective, and environment-friendly fluorescent peptide probe His-His-Trp-His (HHWH) for determining the concentration of copper ion (Cu2+) in aqueous solutions. The results demonstrated that the designed HHWH has a high selectivity and sensitivity for monitoring the concentration of free Cu2+ via quenching of the probe fluorescence upon a binding of Cu2+. The fluorescence intensity of the HHWH had a linear relationship with the concentration of Cu2+ between 10 nM and 10 μM, and the detection limit was 8 nM. Furthermore, HHWH could be regenerated with sulfide ions at least five times. The concentrations of Cu2+ in three different real water samples were detected using this probe, and the results were consistent with the one detected using an atomic absorption spectrometer. Thus, HHWH can be used as an accurate and feasible fluorescent peptide probe for detecting Cu2+ in aqueous solutions.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 81501619). the Grain-Oil Pi■ocess and Quality Control 2011 Collaborative and Innovative Grant from Hunan Province of China, a Natural Science Foundation of Hunan Province of China (No. 2015JJ3I77), a science foundation from the Science and Technology Department of Changsha of China (No. k 1501003-31). the Science Foundation from Education Department of Hunan Province of China (No. 14C1184), the college students’ science and technology innovation project form Education Department of Hunan Province of China (2014. 2015). the initiation funding to Lin Zhang from Central South University for Forestry and Technology, the Special Fund for Agro-scientific Research in (he Public Interest of China (201303071.201303084). the S&T Plan Key Project of Hunan (2013WK4006) and the initiation funding to Tao Yang from Central South University for Forestry and Technology.

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Authors and Affiliations

  1. National Engineering Laboratory for Rice and By-products Further Processing, Central South University of Forestry and Technology, Changsha, 410004, People’s Republic of China

    Tao Yang, Fang Zhu, Tianxiao Zhou, Jianzhong Cao, Yibo Xie, Meiyi Zhang, Yan Wang, Qinlu Lin & Lin Zhang

  2. Department of Food Science and Technology, Central South University of Forestry and Technology, Changsha, 410004, People’s Republic of China

    Tao Yang, Fang Zhu, Tianxiao Zhou, Jianzhong Cao, Yibo Xie, Meiyi Zhang, Yan Wang, Qinlu Lin & Lin Zhang

  3. 'School of Pharmaceutical Sciences, Central South University, Changsha, 410083, People’s Republic of China

    Dong-Sheng Cao

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  1. Tao Yang
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  2. Fang Zhu
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Correspondence to Lin Zhang.

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Yang, T., Zhu, F., Zhou, T. et al. Label-free, Water-soluble Fluorescent Peptide Probe for a Sensitive and Selective Determination of Copper Ions. ANAL. SCI. 33, 191–196 (2017). https://doi.org/10.2116/analsci.33.191

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  • DOI: https://doi.org/10.2116/analsci.33.191

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