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Analysis of Ion Transport through a Single Channel of Gramicidin A in Bilayer Lipid Membranes

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Abstract

Ion transport through a single channel of gramicidin A (GA) within the bilayer lipid membrane (BLM) between two aqueous phases (W1 and W2) has been analyzed based on the electroneutrality principle. The single-channel current increases in proportion to the magnitude of the applied membrane potential and is also dependent on the permeability coefficients of electrolyte ions (K+ and Cl−). By varying the ratio of the concentration of KCl in W1 to that in W2 the ratio of the diffusion coefficient of K+ in the BLM to that of Cl− in the BLM can be evaluated.

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Authors and Affiliations

  1. Division of Applied Life Sciences, Graduate School of Agriculture Kyoto University, Sakyo, Kyoto, 606-8502, Japan

    Shintaro Kubota, Osamu Shirai, Yuki Kitazumi & Kenji Kano

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  1. Shintaro Kubota
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  2. Osamu Shirai
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  3. Yuki Kitazumi
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  4. Kenji Kano
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Correspondence to Osamu Shirai.

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Kubota, S., Shirai, O., Kitazumi, Y. et al. Analysis of Ion Transport through a Single Channel of Gramicidin A in Bilayer Lipid Membranes. ANAL. SCI. 32, 189–192 (2016). https://doi.org/10.2116/analsci.32.189

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  • DOI: https://doi.org/10.2116/analsci.32.189

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